Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)

Zhao-yu Lu; Shu-wen Liu; Yuan-sheng Xie; Shao-yuan Cui; Xu-sheng Liu; Wen-jia Geng; Xiao Hu; Jia-yao Ji; Xiang-mei Chen

doi:10.1007/s11655-013-1654-9

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Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)

Updated：2021-08-27

- Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)
- Chinese Journal of Integrative Medicine Vol. 19, Issue 12, Pages: 918-926(2013)
- Affiliations：
  
  1. Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV)00088, Chinese PLA General Hospital,Beijing,China
  2. Department of Nephrology, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (No. 2011CB944004), the Program of the National Natural Science Foundation of China (No. 30971377) and the Science and Technology Project of Beijing, China (No. D09050704310904);These authors contributed equally to this work
- DOI：10.1007/s11655-013-1654-9
  CLC：
- Published：2013，
  
  Published Online：5 December 2013，
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Lu, Zy., Liu, Sw., Xie, Ys. et al. Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)., Chin. J. Integr. Med. 19, 918–926 (2013). https://doi.org/10.1007/s11655-013-1654-9

Zhao-yu Lu, Shu-wen Liu, Yuan-sheng Xie, et al. Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒). [J]. Chinese Journal of Integrative Medicine 19(12):918-926(2013)
Lu, Zy., Liu, Sw., Xie, Ys. et al. Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)., Chin. J. Integr. Med. 19, 918–926 (2013). https://doi.org/10.1007/s11655-013-1654-9 DOI：

Zhao-yu Lu, Shu-wen Liu, Yuan-sheng Xie, et al. Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒). [J]. Chinese Journal of Integrative Medicine 19(12):918-926(2013) DOI： 10.1007/s11655-013-1654-9.

摘要

To investigate the effect of the Uremic Clearance Granule (UCG

尿毒清颗粒)

a Chinese patent medicine

on tubular epithelial-to-mesenchymal transition (EMT) in a unilateral ureteral obstruction (UUO) model in vivo and transforming growth factor (TGF)-β1 induced EMT of HK-2 cells in vitro. In vivo study

50 Sprague Dawley rats were divided into three groups: a sham operation group (n=10)

a UUO group (n=20)

and a UUO with UCG treatment group (n=20). The UCG was given at a dose of 4.5 g/kg body weight per day by gavage after surgery. In vitro study

HK-2 cells were cultured in 10% fetal bovine serum (FBS)

10% healthy rat serum

10% FBS and TGF-β1 (10 ng/mL)

10% healthy rat serum and TGF-β1

or 10% rat serum containing the uremic clearance granule and TGF-β1. The expression of the epithelial marker E-cadherin and the mesenchymal markers vimentin and α-smooth muscle actin (α-SMA) in kidney tissues and HK-2 cells were investigated by Western blot analysis and immunofluorescence staining. The rats of the UUO group showed obvious tubulointerstitial fibrosis

compared with the sham operation group rats. Tubulointerstitial fibrosis score was reduced by 17.5%±1.1% at day 7 and by 20.0%±1.2% at day 14 in the UCG-treated group

compared with the UUO group. The UCG could maintained expression of E-cadherin and suppressed expression of vimentin and α-SMA in kidney tissues of UUO rats at days 7 and 14

as determined by Western blot analysis and immunofluorescence staining. Rat serum containing the UCG partially inhibited TGF-β1-induced fibroblast phenotype of HK-2 cells and maintained the epithelial morphology of HK-2 cells in vitro. This occurred partially through a reduction of vimentin expression and an increase of E-cadherin expression. These results suggest that the UCG prevents tubular EMT and may be a promising agent for treating tubulointerstitial fibrosis.

Abstract

To investigate the effect of the Uremic Clearance Granule (UCG

尿毒清颗粒)

a Chinese patent medicine

50 Sprague Dawley rats were divided into three groups: a sham operation group (n=10)

a UUO group (n=20)

and a UUO with UCG treatment group (n=20). The UCG was given at a dose of 4.5 g/kg body weight per day by gavage after surgery. In vitro study

HK-2 cells were cultured in 10% fetal bovine serum (FBS)

10% healthy rat serum

10% FBS and TGF-β1 (10 ng/mL)

10% healthy rat serum and TGF-β1

compared with the sham operation group rats. Tubulointerstitial fibrosis score was reduced by 17.5%±1.1% at day 7 and by 20.0%±1.2% at day 14 in the UCG-treated group

compared with the UUO group. The UCG could maintained expression of E-cadherin and suppressed expression of vimentin and α-SMA in kidney tissues of UUO rats at days 7 and 14

关键词

Uremic Clearance Granuleepithelial-to-mesenchymal transitiontubulointerstitial fibrosis

Keywords

Uremic Clearance Granuleepithelial-to-mesenchymal transitiontubulointerstitial fibrosis

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