Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression

Xi-li Wu; Peng An; Bing-yu Ye; Xing-min Shi; Wan-sen Sun; Rong-guo Fu; Zhu Wang; Cheng-lin Qiao

doi:10.1007/s11655-013-1655-8

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Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression

Updated：2021-08-27

- Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression
- Chinese Journal of Integrative Medicine Vol. 19, Issue 12, Pages: 927-934(2013)
- Affiliations：
  
  1. Department of Integrated Traditional Chinese and Western Medicine, Second Affiliated Hospital of Medical School of Xi’an Jiaotong University,Xi’an,China
  2. School of Public Health of Xi’an Jiaotong University,Xi’an,China
  3. Department of Nephrology, Second Affiliated Hospital of Medical School of Xi’an Jiaotong University,Xi’an,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30801504, No. 30901926, No. 81100530 and No. 81070590)
- DOI：10.1007/s11655-013-1655-8
  CLC：
- Published：2013，
  
  Published Online：5 December 2013，
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Wu, Xl., An, P., Ye, By. et al. Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression., Chin. J. Integr. Med. 19, 927–934 (2013). https://doi.org/10.1007/s11655-013-1655-8

Xi-li Wu, Peng An, Bing-yu Ye, et al. Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression. [J]. Chinese Journal of Integrative Medicine 19(12):927-934(2013)
Wu, Xl., An, P., Ye, By. et al. Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression., Chin. J. Integr. Med. 19, 927–934 (2013). https://doi.org/10.1007/s11655-013-1655-8 DOI：

Xi-li Wu, Peng An, Bing-yu Ye, et al. Qufeng Tongluo Prescription (祛风通络方) inhibits mesangial cell proliferation and promotes apoptosis through regulating cell cycle progression. [J]. Chinese Journal of Integrative Medicine 19(12):927-934(2013) DOI： 10.1007/s11655-013-1655-8.

摘要

To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription (祛风通络方

QFTL) on the regulation of mesangial cells (MCs) proliferation and apoptosis. The MCs used in this experiment have undergone five passages induced by lipopolysaccharide (LPS). Changes in the proliferation

apoptosis

cell cycle regulatory proteins and mRNA expression levels of the MCs after administration of Benazepril or QFTL were measured by methyl thiazolyl tetrazolium (MTT) reduction assay

flow cytometry

Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)

respectively. The addition of Benazepril or QFTL serum inhibited LPS-induced MC proliferation after treatment for 24

48 and 72 h

respectively (P<0.05 or P<0.01). Moreover

the inhibitory effect is more significant in the QFTL group at 48 h (P<0.05). Compared with the control group

LPS-induced cell proliferation decreased the number of cells in G1 phase versus cells in S and G2/M phases

while the addition of QFTL and Benazepril serum increased the ratio of cells at G1 phase (P<0.05 or P<0.01) to cells at S phase (P<0.01)

implicating the cell cycle inhibition effect exerted by QFTL. LPS decreased the level of MC apoptosis

compared with the control group (P<0.05)

while QFTL and Benazepril serum increased the level of MC apoptosis (P<0.01). Moreover

the difference between the QFTL group and the Benazepril group was statistically significant (P<0.01). Compared with the control group

the protein and mRNA expression levels of cylinD1

cyclin dependent kinase 2 (CDK2) and p21 were significantly increased (P<0.05 or P<0.01)

p27 was decreased but with no statistical significance (P>0.05); After being treated with QFTL and Benazepril serum

the protein and mRNA expression levels of cylinD1

CDK2

p21 were decreased and p27 increased significantly (P<0.05 or P<0.01); Compared with the Benazepril group

QFTL show better effects on protein and mRNA expression levels of cylinD1

CDK2 (P<0.05 or P<0.01) and p21 protein expression (P<0.05). QFTL inhibits MCs proliferation

promotes MCs apoptosis through an underlying mechanism of down-regulating the protein and mRNA expression levels of cylinD1

CDK2

p21 and up-regulation of the expression level of p27.

Abstract

To study the effects and possible underlying mechanism of Qufeng Tongluo Prescription (祛风通络方

apoptosis

cell cycle regulatory proteins and mRNA expression levels of the MCs after administration of Benazepril or QFTL were measured by methyl thiazolyl tetrazolium (MTT) reduction assay

flow cytometry

Western blot and quantitative real-time polymerase chain reaction (qRT-PCR)

respectively. The addition of Benazepril or QFTL serum inhibited LPS-induced MC proliferation after treatment for 24

48 and 72 h

respectively (P<0.05 or P<0.01). Moreover

the inhibitory effect is more significant in the QFTL group at 48 h (P<0.05). Compared with the control group

LPS-induced cell proliferation decreased the number of cells in G1 phase versus cells in S and G2/M phases

while the addition of QFTL and Benazepril serum increased the ratio of cells at G1 phase (P<0.05 or P<0.01) to cells at S phase (P<0.01)

implicating the cell cycle inhibition effect exerted by QFTL. LPS decreased the level of MC apoptosis

compared with the control group (P<0.05)

while QFTL and Benazepril serum increased the level of MC apoptosis (P<0.01). Moreover

the difference between the QFTL group and the Benazepril group was statistically significant (P<0.01). Compared with the control group

the protein and mRNA expression levels of cylinD1

cyclin dependent kinase 2 (CDK2) and p21 were significantly increased (P<0.05 or P<0.01)

p27 was decreased but with no statistical significance (P>0.05); After being treated with QFTL and Benazepril serum

the protein and mRNA expression levels of cylinD1

CDK2

p21 were decreased and p27 increased significantly (P<0.05 or P<0.01); Compared with the Benazepril group

QFTL show better effects on protein and mRNA expression levels of cylinD1

CDK2 (P<0.05 or P<0.01) and p21 protein expression (P<0.05). QFTL inhibits MCs proliferation

promotes MCs apoptosis through an underlying mechanism of down-regulating the protein and mRNA expression levels of cylinD1

CDK2

p21 and up-regulation of the expression level of p27.

关键词

Qufeng Tongluo Prescriptionmesangial cellscell proliferationcell apoptosiscell cyclecell cycle regulatory proteins

Keywords

Qufeng Tongluo Prescriptionmesangial cellscell proliferationcell apoptosiscell cyclecell cycle regulatory proteins

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