Modified Xiaochaihu Decoction (小柴胡汤) prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway

Shu-kun Zhang; Nai-qiang Cui; Yu-zhen Zhuo; Di-hua Li; Jun-hong Liu

doi:10.1007/s11655-013-1656-7

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Modified Xiaochaihu Decoction (小柴胡汤) prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway

Updated：2021-08-27

- Modified Xiaochaihu Decoction (小柴胡汤) prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway
- Chinese Journal of Integrative Medicine Vol. 19, Issue 12, Pages: 935-939(2013)
- Affiliations：
  
  Tianjin Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine,Tianjin,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81102686)
- DOI：10.1007/s11655-013-1656-7
  CLC：
- Published：2013，
  
  Published Online：5 December 2013，
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Zhang, Sk., Cui, Nq., Zhuo, Yz. et al. Modified Xiaochaihu Decoction (小柴胡汤) prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway., Chin. J. Integr. Med. 19, 935–939 (2013). https://doi.org/10.1007/s11655-013-1656-7

Shu-kun Zhang, Nai-qiang Cui, Yu-zhen Zhuo, et al. Modified Xiaochaihu Decoction (小柴胡汤) prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway. [J]. Chinese Journal of Integrative Medicine 19(12):935-939(2013)
Zhang, Sk., Cui, Nq., Zhuo, Yz. et al. Modified Xiaochaihu Decoction (小柴胡汤) prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway., Chin. J. Integr. Med. 19, 935–939 (2013). https://doi.org/10.1007/s11655-013-1656-7 DOI：

Shu-kun Zhang, Nai-qiang Cui, Yu-zhen Zhuo, et al. Modified Xiaochaihu Decoction (小柴胡汤) prevents the progression of chronic pancreatitis in rats possibly by inhibiting transforming growth factor-β1/Sma- and mad-related proteins signaling pathway. [J]. Chinese Journal of Integrative Medicine 19(12):935-939(2013) DOI： 10.1007/s11655-013-1656-7.

摘要

To investigate the effect of modified Xiaochaihu Decoction (小柴胡汤

MXD) on transforming growth factor-β1/Sma- and Mad-related proteins (TGF-β1/Smads) signaling pathway in rats with chronic pancreatitis (CP) induced by dibutyltin dichloride. Thirty healthy male Wistar rats were randomly divided into the normal control group

CP group and CP+MXD-treated group. CP was induced by injection of dibutyltin dichloride (DBTC

7 mg/kg of body weight) into the right caudal vein

and the control rats were treated with vehicle. MXD was given daily by gavage at a dose of 10 g/kg of body weight

starting from the day after CP induction. After 28-day treatment

the n-benzoyl-tyrosyl para-aminobenzoic acid (NBT-PABA) test was carried out to evaluate exocrine pancreatic function. Then

rats were sacrificed

and pancreatic tissues were harvested for histological evaluation. In addition

the mRNA expression of TGF-β1

TGF-β1 type II receptor (TGFβRII)

Smad3 and Smad7 was determined in pancreatic tissues by using real-time polymerase chain reaction. Treatment of CP with MXD improved the PABA recovery

decreased the histological lesion

and reduced the mRNA expression of TGF-β1

TGFβRII and Smad3 (P<0.05). However

MXD had no effect on Smad7 mRNA level. MXD could protect the pancreas against chronic injury and improve pancreatic exocrine function in DBTC induced rat CP model. Its mechanism may involve inhibition of the TGF-β1/Smads signaling pathway.

Abstract

To investigate the effect of modified Xiaochaihu Decoction (小柴胡汤

CP group and CP+MXD-treated group. CP was induced by injection of dibutyltin dichloride (DBTC

7 mg/kg of body weight) into the right caudal vein

and the control rats were treated with vehicle. MXD was given daily by gavage at a dose of 10 g/kg of body weight

starting from the day after CP induction. After 28-day treatment

the n-benzoyl-tyrosyl para-aminobenzoic acid (NBT-PABA) test was carried out to evaluate exocrine pancreatic function. Then

rats were sacrificed

and pancreatic tissues were harvested for histological evaluation. In addition

the mRNA expression of TGF-β1

TGF-β1 type II receptor (TGFβRII)

Smad3 and Smad7 was determined in pancreatic tissues by using real-time polymerase chain reaction. Treatment of CP with MXD improved the PABA recovery

decreased the histological lesion

and reduced the mRNA expression of TGF-β1

TGFβRII and Smad3 (P<0.05). However

关键词

modified Xiaochaihu Decoctiontransforming growth factor-β1/Sma- and Mad-related proteinschronic pancreatitis

Keywords

modified Xiaochaihu Decoctiontransforming growth factor-β1/Sma- and Mad-related proteinschronic pancreatitis

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