Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines

Chang-Nam Ko; In-Seo Park; Seong-Uk Park; Woo-Sang Jung; Sang-Kwan Moon; Jung-Mi Park; Chulhun Kang; Ki-Ho Cho

doi:10.1007/s11655-013-1657-6

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Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines

Updated：2021-08-27

- Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines
- Chinese Journal of Integrative Medicine Vol. 19, Issue 12, Pages: 940-944(2013)
- Affiliations：
  
  1. Department of Cardiology and Neurology Diseases, College of Korean Medicine, Kyung Hee University,Seoul,Korea
  2. Department of Medical Science, Graduate School of East-West Medical Science, Kyung Hee University,Yongin,Korea
- Author bio：
- Funds：
- DOI：10.1007/s11655-013-1657-6
  CLC：
- Published：2013，
  
  Published Online：5 December 2013，
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Ko, CN., Park, IS., Park, SU. et al. Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines., Chin. J. Integr. Med. 19, 940–944 (2013). https://doi.org/10.1007/s11655-013-1657-6

Chang-Nam Ko, In-Seo Park, Seong-Uk Park, et al. Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines. [J]. Chinese Journal of Integrative Medicine 19(12):940-944(2013)
Ko, CN., Park, IS., Park, SU. et al. Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines., Chin. J. Integr. Med. 19, 940–944 (2013). https://doi.org/10.1007/s11655-013-1657-6 DOI：

Chang-Nam Ko, In-Seo Park, Seong-Uk Park, et al. Neuroprotective effect of Chunghyuldan (Qing Xue Dan) on hypoxia-reoxygenation induced damage of neuroblastoma 2a cell lines. [J]. Chinese Journal of Integrative Medicine 19(12):940-944(2013) DOI： 10.1007/s11655-013-1657-6.

摘要

Chunghyuldan (CHD)

a combinatorial drug that has anti-hyperlipidemic and antiinflammatory activities

has been shown to reduce infarct volume in a focal ischemia-reperfusion rat model. To explore the molecular basis of CHD’s neuroprotective effect

we examined whether CHD shows a cell-protective activity and has a regulatory effect on Bax and/or B-cell leukemia/lymphoma 2 (Bcl-2) expression in mouse neuroblastoma 2a (N2a) cells subjected to hypoxia-reoxygenation (H/R). In order to evaluate the effects of CHD on the cytotoxicity induced from hypoxia or H/R condition

lactate dehydrogenase (LDH) assay was performed. To explore whether the suppression of neural damage when pre-treated with CHD is associated with its anti-apoptotic effect

the CHD effect on the expression of Bcl-2 and Bax was analyzed by Western blotting analysis. Cytotoxicity of N2a cell line was slightly increased in 42 h hypoxia condition and dramatically increased under the H/R condition. CHD treatment markedly decreased the cytotoxicity in both conditions (P<0.01

P<0.05). H/R markedly increased the expression of the pro-apoptotic protein

Bax

but slightly increased the expression of the anti-apoptotic protein

Bcl-2

compared with the normoxia or hypoxia group. CHD significantly decreased Bax expression (P<0.01) and slightly decreased Bcl-2 expression (P>0.05)

resulted in a reduction of Bax/Bcl-2 ratio in N2a cells subjected to H/R. CHD has neuroprotective effect in N2a cells subjected to H/R

which might be derived at least in part from its ability to decrease the expression of the pro-apoptotic protein

Bax.

Abstract

Chunghyuldan (CHD)

a combinatorial drug that has anti-hyperlipidemic and antiinflammatory activities

has been shown to reduce infarct volume in a focal ischemia-reperfusion rat model. To explore the molecular basis of CHD’s neuroprotective effect

lactate dehydrogenase (LDH) assay was performed. To explore whether the suppression of neural damage when pre-treated with CHD is associated with its anti-apoptotic effect

P<0.05). H/R markedly increased the expression of the pro-apoptotic protein

Bax

but slightly increased the expression of the anti-apoptotic protein

Bcl-2

compared with the normoxia or hypoxia group. CHD significantly decreased Bax expression (P<0.01) and slightly decreased Bcl-2 expression (P>0.05)

resulted in a reduction of Bax/Bcl-2 ratio in N2a cells subjected to H/R. CHD has neuroprotective effect in N2a cells subjected to H/R

which might be derived at least in part from its ability to decrease the expression of the pro-apoptotic protein

Bax.

关键词

apoptosisstrokeChunghyuldanherbal

Keywords

apoptosisstrokeChunghyuldanherbal

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