Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)

Ping Xue; Jia Guo; Xiao-nan Yang; Wei Huang; Qing Xia

doi:10.1007/s11655-013-1661-x

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Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)

Updated：2021-08-27

- Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)
- Chinese Journal of Integrative Medicine Vol. 20, Issue 10, Pages: 770-775(2014)
- Affiliations：
  
  1. Pancreatic Diseases Research Group, Department of Integrated Traditional and Western Medicine, West China Hospital, Sichuan University,Chengdu,China
  2. Liverpool NIHR Pancreas Biomedical Research Unit, Royal Liverpool University Hospital, University of Liverpool,Liverpool,UK
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81072910) and Science and Technology Support Program of Sichuan (No. 2011SZ0291, 2010SZ0068)
- DOI：10.1007/s11655-013-1661-x
  CLC：
- Published：2014，
  
  Published Online：23 January 2014，
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Xue, P., Guo, J., Yang, Xn. et al. Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)., Chin. J. Integr. Med. 20, 770–775 (2014). https://doi.org/10.1007/s11655-013-1661-x

Ping Xue, Jia Guo, Xiao-nan Yang, et al. Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤). [J]. Chinese Journal of Integrative Medicine 20(10):770-775(2014)
Xue, P., Guo, J., Yang, Xn. et al. Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)., Chin. J. Integr. Med. 20, 770–775 (2014). https://doi.org/10.1007/s11655-013-1661-x DOI：

Ping Xue, Jia Guo, Xiao-nan Yang, et al. Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤). [J]. Chinese Journal of Integrative Medicine 20(10):770-775(2014) DOI： 10.1007/s11655-013-1661-x.

摘要

To investigate effect of Chaiqin Chengqi Decoction (柴芩承气汤

CQCQD) on changes of neuronal acetylcholine receptor alpha 7 (nAChRα7) of peritoneal macrophages in acute pancreatitis (AP). Eighteen Kunming mice were equally randomized into the control group

AP group and CQCQD treatment group. AP was induced by two intraperitoneal injections of 4 g/kg L-arginine at 1 h apart

while control mice received saline injections. At 72 h after the first injection of L-arginine

mice in the treatment group were intragastrically administered 0.1 mL/10 g CQCQD every 2 h for 3 times

whilst mice in the other two groups received the same amount of saline feeding. Mice were sacrificed by cervical dislocation 2 h after the last feeding of either CQCQD or saline. Peritoneal macrophages were collected for determination of nAChRα7 mRNA and protein expression. Serum was collected for detection of interleukin-6 (IL-6)

IL-10 and acetylcholine (ACh) levels

and pancreas was for histopathology analysis. The CQCQD treatment significantly ameliorated the severity of AP as evidenced by reducing the pancreatic histopathology score (4.5±0.5 vs. 6.2±1.7

P<0.05) and the serum IL-6 levels (1228.3±419.2 pg/mL vs. 1589.6±337.3 pg/mL

P<0.05). The mRNA and protein expression of nAChRα7 of the peritoneal macrophages in the AP group were similar to the control group (P>0.05)

but were significantly up-regulated after the CQCQD treatment (P<0.05). The serum ACh levels in the AP group were significantly lower than those in the control group (3.1±0.6 μg/mL vs 4.8±0.7 μg/mL P<0.05)

but were significantly increased after the CQCQD treatment (5.6±1.5 μg/mL vs 3.1±0.6 μg/mL

P<0.05). CQCQD is protective against L-arginine-induced AP through mechanisms involving nAChRα7 of peritoneal macrophages.

Abstract

To investigate effect of Chaiqin Chengqi Decoction (柴芩承气汤

CQCQD) on changes of neuronal acetylcholine receptor alpha 7 (nAChRα7) of peritoneal macrophages in acute pancreatitis (AP). Eighteen Kunming mice were equally randomized into the control group

AP group and CQCQD treatment group. AP was induced by two intraperitoneal injections of 4 g/kg L-arginine at 1 h apart

while control mice received saline injections. At 72 h after the first injection of L-arginine

mice in the treatment group were intragastrically administered 0.1 mL/10 g CQCQD every 2 h for 3 times

IL-10 and acetylcholine (ACh) levels

and pancreas was for histopathology analysis. The CQCQD treatment significantly ameliorated the severity of AP as evidenced by reducing the pancreatic histopathology score (4.5±0.5 vs. 6.2±1.7

P<0.05) and the serum IL-6 levels (1228.3±419.2 pg/mL vs. 1589.6±337.3 pg/mL

P<0.05). The mRNA and protein expression of nAChRα7 of the peritoneal macrophages in the AP group were similar to the control group (P>0.05)

but were significantly increased after the CQCQD treatment (5.6±1.5 μg/mL vs 3.1±0.6 μg/mL

P<0.05). CQCQD is protective against L-arginine-induced AP through mechanisms involving nAChRα7 of peritoneal macrophages.

关键词

acetylcholineAcute PancreatitisChaiqin Chengqi Decoctioncholinergic anti-inflammatory pathwayneuronal acetylcholine receptor alpha 7

Keywords

acetylcholineAcute PancreatitisChaiqin Chengqi Decoctioncholinergic anti-inflammatory pathwayneuronal acetylcholine receptor alpha 7

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Hepatobiliary Department, Panzhihua Integrated Traditional and Western Medicine Hospital

Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University

Department of Integrated Chinese and Western Medicine, West China Hospital of Sichuan University

Department of General Surgery, West China Hospital, Sichuan University

Luo Jun Dept. of Pharmacology, Guiyang Medical College

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