Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior, monoamine neurotransmitter levels, and serotonin receptor subtype expression in the brain of a rat depression model

Shi-jing Huang; Xian-hui Zhang; Yan-yun Wang; Ju-hua Pan; Han-ming Cui; Su-ping Fang; Wei Wu; Jun Zheng; Duo-jiao Li; Ge Bai

doi:10.1007/s11655-014-1343-0

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Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior, monoamine neurotransmitter levels, and serotonin receptor subtype expression in the brain of a rat depression model

Updated：2021-08-27

- Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior, monoamine neurotransmitter levels, and serotonin receptor subtype expression in the brain of a rat depression model
- Chinese Journal of Integrative Medicine Vol. 20, Issue 4, Pages: 280-285(2014)
- Affiliations：
  
  Traditional Chinese Medicine Research and Development Center, Guang’anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30672696 and 81072801)
- DOI：10.1007/s11655-014-1343-0
  CLC：
- Published：2014，
  
  Published Online：24 February 2014，
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Huang, Sj., Zhang, Xh., Wang, Yy. et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior, monoamine neurotransmitter levels, and serotonin receptor subtype expression in the brain of a rat depression model., Chin. J. Integr. Med. 20, 280–285 (2014). https://doi.org/10.1007/s11655-014-1343-0

Shi-jing Huang, Xian-hui Zhang, Yan-yun Wang, et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior, monoamine neurotransmitter levels, and serotonin receptor subtype expression in the brain of a rat depression model. [J]. Chinese Journal of Integrative Medicine 20(4):280-285(2014)
Huang, Sj., Zhang, Xh., Wang, Yy. et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior, monoamine neurotransmitter levels, and serotonin receptor subtype expression in the brain of a rat depression model., Chin. J. Integr. Med. 20, 280–285 (2014). https://doi.org/10.1007/s11655-014-1343-0 DOI：

Shi-jing Huang, Xian-hui Zhang, Yan-yun Wang, et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior, monoamine neurotransmitter levels, and serotonin receptor subtype expression in the brain of a rat depression model. [J]. Chinese Journal of Integrative Medicine 20(4):280-285(2014) DOI： 10.1007/s11655-014-1343-0.

摘要

To determine the mechanisms underlying the anti-depressant effects of Kaixin Jieyu Decoction (开心解郁汤

KJD) by investigating the effects of KJD on behavior

monoamine neurotransmitter levels

and serotonin (5-HT) receptor subtype expression in the brain in a rat model of depression. The rat depression model was established using chronic unpredictable mild stress (CUMS). Forty-eight Sprague Dawley rats were randomly divided into control

depression model (CUMS)

CUMS+KJD (7.7 g/kg−1·d−1 of crude drug)

and CUMS+fluoxetine (2.4 mg/kg−1·d−1) groups (n=12 in each group)

and the treatments lasted for 21 days. We regularly evaluated body weight

sucrose consumption

and horizontal and vertical activity scores in open-field tests. The content of the monoamine neurotransmitters 5-HT

norepinephrine (NE)

and dopamine (DA) and the DA metabolite homovanillic acid in the cerebral cortex

and 5-HT1A and 5-HT2A receptor mRNA in the cerebral cortex and the hippocampus

were determined respectively by high-performance liquid chromatography-coularray electrochemical detector and real-time polymerase chain reaction. Compared with the control group

CUMS rats showed a variety of depression-like behavioral changes

including a significant reduction in body weight

sucrose consumption

and horizontal and vertical activity scores in open-field tests (P<0.05 or P<0.01)

and a significant decrease in 5-HT and NE levels and 5-HT2A receptor mRNA expression. In contrast

they showed a significant increase in 5-HT1A receptor mRNA expression in the cerebral cortex. In the hippocampus

5-HT1A receptor mRNA expression was lower whereas 5-HT2A receptor mRNA expression was higher than in the control group (P<0.05 or P<0.01). Treatment with KJD or fluoxetine partially attenuated these changes (P<0.05 or P<0.01). KJD could normalize the levels of 5-HT and NE and adjust the balance of 5-HT1A and 5-HT2A receptor expression in rat cerebrum

and this may be one of mechanisms of antidepressant effects of KJD.

Abstract

To determine the mechanisms underlying the anti-depressant effects of Kaixin Jieyu Decoction (开心解郁汤

KJD) by investigating the effects of KJD on behavior

monoamine neurotransmitter levels

depression model (CUMS)

CUMS+KJD (7.7 g/kg−1·d−1 of crude drug)

and CUMS+fluoxetine (2.4 mg/kg−1·d−1) groups (n=12 in each group)

and the treatments lasted for 21 days. We regularly evaluated body weight

sucrose consumption

and horizontal and vertical activity scores in open-field tests. The content of the monoamine neurotransmitters 5-HT

norepinephrine (NE)

and dopamine (DA) and the DA metabolite homovanillic acid in the cerebral cortex

and 5-HT1A and 5-HT2A receptor mRNA in the cerebral cortex and the hippocampus

were determined respectively by high-performance liquid chromatography-coularray electrochemical detector and real-time polymerase chain reaction. Compared with the control group

CUMS rats showed a variety of depression-like behavioral changes

including a significant reduction in body weight

sucrose consumption

and horizontal and vertical activity scores in open-field tests (P<0.05 or P<0.01)

and a significant decrease in 5-HT and NE levels and 5-HT2A receptor mRNA expression. In contrast

they showed a significant increase in 5-HT1A receptor mRNA expression in the cerebral cortex. In the hippocampus

and this may be one of mechanisms of antidepressant effects of KJD.

关键词

depressionKaixin Jieyu Decoctionbehaviormonoamines neurotransmitterserotonin1A and serotonin2A receptor mRNA

Keywords

depressionKaixin Jieyu Decoctionbehaviormonoamines neurotransmitterserotonin1A and serotonin2A receptor mRNA

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Jiangsu Hospital of Chinese Medicine

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The Second Clinical College of Nanjing University of Chinese Medicine

Basic Medical College of Nanjing University of Chinese Medicine

Institute of Neurology, Anhui University of Traditional Chinese Medicine

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