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Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro
- Chinese Journal of Integrative Medicine Vol. 22, Issue 5, Pages: 377-383(2016)
- Affiliations：
1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz,Iran
2. Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, Tabriz,Iran
3. Department of Physiology, Tabriz University of Medical Sciences, Tabriz,Iran
4. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,Iran
5. Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad,Iran
6. Department of Basic sciences, Islamic Azad University, Tabriz branch, Tabriz,Iran
- Author bio：
- Funds：
DOI：10.1007/s11655-014-1623-3
CLC：Published：2016，
Published Online：26 August 2014，
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Keyhanmanesh, R., Boskabady, M.H., Saadatloo, M.A.E. et al. Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro, ., Chin. J. Integr. Med. 22, 377–383 (2016). https://doi.org/10.1007/s11655-014-1623-3
Rana Keyhanmanesh, Mohammad Hossien Boskabady, Mohammad Ali Ebrahimi Saadatloo, et al. Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro. [J]. Chinese Journal of Integrative Medicine 22(5):377-383(2016)
Keyhanmanesh, R., Boskabady, M.H., Saadatloo, M.A.E. et al. Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro, ., Chin. J. Integr. Med. 22, 377–383 (2016). https://doi.org/10.1007/s11655-014-1623-3 DOI：
Rana Keyhanmanesh, Mohammad Hossien Boskabady, Mohammad Ali Ebrahimi Saadatloo, et al. Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro. [J]. Chinese Journal of Integrative Medicine 22(5):377-383(2016) DOI： 10.1007/s11655-014-1623-3.
摘要
To examine the relaxant effects of hydro-ethanolic
macerated aqueous (MA) and lipidfree macerated aqueous (LFMA) extract of Tymus vulgaris on tracheal chains of guinea pigs. The relaxant effects of five cumulative concentrations of each extract (0.4
0.8
1.2
1.6 and 2.0 g/100 mL) were compared with saline as negative control and five cumulative concentrations of theophylline (0.1
0.2
0.4
0.6 and 0.8 mmol/L) on precontracted tracheal smooth muscle of guinea pig with 60 mmol/L KCl (group 1) and 10 µmol/L methacholine (group 2
n=6 for each group). In group 1 all concentrations of theophylline
three higher concentrations of hydro-ethanolic
two concentrations of LFMA and last concentration of MA extracts showed significant relaxant effects compared with that of saline (P<0.05 or P<0.01). Two lower concentrations of LFMA and all concentrations of MA except higher one caused contraction compared with saline (P<0.05 or 0.01). In group 2 experiments
all concentrations of theophylline
hydro-ethanolic
MA and LFMA extracts showed significant relaxant effects compared to that of saline (P<0.05 or P<0.01). In both groups
the relaxant effect of all concentrations of hydro-ethanolic extract were significantly higher than most concentrations of others (P<0.05 or P<0.01). The relaxant effect of different concentrations of three extracts were significantly greater in group 2 compared with group 1 experiments (all P<0.01). There were significantly positive correlations between the relaxant effects and concentrations for theophylline and all extracts in both groups (P<0.05 or P<0.01). Hydro-ethanolic extract has a potent weaker relaxant effect for other extracts from Tymus vulgaris on tracheal chains of guinea pigs.
Abstract
To examine the relaxant effects of hydro-ethanolic
macerated aqueous (MA) and lipidfree macerated aqueous (LFMA) extract of Tymus vulgaris on tracheal chains of guinea pigs. The relaxant effects of five cumulative concentrations of each extract (0.4
0.8
1.2
1.6 and 2.0 g/100 mL) were compared with saline as negative control and five cumulative concentrations of theophylline (0.1
0.2
0.4
0.6 and 0.8 mmol/L) on precontracted tracheal smooth muscle of guinea pig with 60 mmol/L KCl (group 1) and 10 µmol/L methacholine (group 2
n=6 for each group). In group 1 all concentrations of theophylline
three higher concentrations of hydro-ethanolic
two concentrations of LFMA and last concentration of MA extracts showed significant relaxant effects compared with that of saline (P<0.05 or P<0.01). Two lower concentrations of LFMA and all concentrations of MA except higher one caused contraction compared with saline (P<0.05 or 0.01). In group 2 experiments
all concentrations of theophylline
hydro-ethanolic
MA and LFMA extracts showed significant relaxant effects compared to that of saline (P<0.05 or P<0.01). In both groups
the relaxant effect of all concentrations of hydro-ethanolic extract were significantly higher than most concentrations of others (P<0.05 or P<0.01). The relaxant effect of different concentrations of three extracts were significantly greater in group 2 compared with group 1 experiments (all P<0.01). There were significantly positive correlations between the relaxant effects and concentrations for theophylline and all extracts in both groups (P<0.05 or P<0.01). Hydro-ethanolic extract has a potent weaker relaxant effect for other extracts from Tymus vulgaris on tracheal chains of guinea pigs.
关键词
Tymus vulgarishydro-ethanolic extractmacerated aqueous extractlipid-free macerated aqueous extractrelaxant effectguinea pigtracheal
Keywords
Tymus vulgarishydro-ethanolic extractmacerated aqueous extractlipid-free macerated aqueous extractrelaxant effectguinea pigtracheal
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