Effects of Compound Shenhua Tablet (复方肾华片) on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury

Yue Yang; Ri-bao Wei; Xiao-yong Zheng; Qiang Qiu; Shao-yuan Cui; Zhong Yin; Suo-zhu Shi; Xiang-mei Chen

doi:10.1007/s11655-014-1740-7

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Effects of Compound Shenhua Tablet (复方肾华片) on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury

Updated：2021-08-27

- Effects of Compound Shenhua Tablet (复方肾华片) on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury
- Chinese Journal of Integrative Medicine Vol. 20, Issue 3, Pages: 200-208(2014)
- Affiliations：
  
  1. State Discipline and State Key Laboratory of Kidney Disease (Chinese PLA General Hospital, 2011DAV),Beijing,China,00088
  2. Medical School of Nankai University,Tianjin,China
- Author bio：
- Funds：
  
  Supported by National Science and Major Project (No. 2010ZX09102-204), National Natural Sciences Foundation of China (No. 81072914 and 81273968), and the Medicine and Health Foundation of PLA (No. 10ZYZ255)
- DOI：10.1007/s11655-014-1740-7
  CLC：
- Published：2014，
  
  Published Online：24 January 2014，
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Yang, Y., Wei, Rb., Zheng, Xy. et al. Effects of Compound Shenhua Tablet (复方肾华片) on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury., Chin. J. Integr. Med. 20, 200–208 (2014). https://doi.org/10.1007/s11655-014-1740-7

Yue Yang, Ri-bao Wei, Xiao-yong Zheng, et al. Effects of Compound Shenhua Tablet (复方肾华片) on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury. [J]. Chinese Journal of Integrative Medicine 20(3):200-208(2014)
Yang, Y., Wei, Rb., Zheng, Xy. et al. Effects of Compound Shenhua Tablet (复方肾华片) on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury., Chin. J. Integr. Med. 20, 200–208 (2014). https://doi.org/10.1007/s11655-014-1740-7 DOI：

Yue Yang, Ri-bao Wei, Xiao-yong Zheng, et al. Effects of Compound Shenhua Tablet (复方肾华片) on renal tubular Na+-K+-ATPase in rats with acute ischemic reperfusion injury. [J]. Chinese Journal of Integrative Medicine 20(3):200-208(2014) DOI： 10.1007/s11655-014-1740-7.

摘要

To observe the effect of Compound Shenhua Tablet (复方肾华片

SHT) on the sodiumpotassium- exchanging adenosinetriphosphatase (Na+-K+-ATPase) in the renal tubular epithelial cells of rats with acute ischemic reperfusion and to investigate the mechanisms underlying the effects of SHT on renal ischemic reperfusion injury (RIRI). Fifty male Wistar rats were randomly divided into the sham surgery group

model group

astragaloside group [150 mg/(kg·d)]

SHT low-dose group [1.5 g/(kg·d)] and SHT high-dose group [3.0 g/(kg·d)]

with 10 rats in each group. After 1 week of continuous intragastric drug administration

surgery was performed to establish the model. At either 24 or 72 h after the surgery

5 rats in each group were sacrificed

blood biochemistry

renal pathology

immunoblot and immunohistochemical examinations were performed

and double immunofluorescence staining was observed under a laser confocal microscope. Compared with the sham surgery group

the serum creatinine (SCr) and blood urea nitrogen (BUN) levels were significantly increased

Na+-K+-ATPase protein level was decreased

and kidney injury molecule-1 (KIM-1) protein level was increased in the model group after the surgery (P<0.01 or P<0.05). Compared with the model group

the SCr

BUN

pathological scores

Na+-K+-ATPase

and the KIM-1 protein level of the three treatment groups were significantly improved at 72 h after the surgery (P<0.05 or P<0.01). And the SCr

BUN of the SHT low- and high-dose groups

and the pathological scores of the SHT high-dose group were significantly lower than those of the astragaloside group (P<0.05). The localizations of Na+-K+-ATPase and megalin of the model group were disrupted

with the distribution areas overlapping with each other and alternately arranged. The severity of the disruption was slightly milder in three treatment groups compared with that of the model group. The results of immunofluorescence staining showed that the SHT high-dose group had a superior effect as compared with the astragaloside group and the SHT low-dose group. The SHT effectively alleviated RIRI caused by ischemic reperfusion

promoted the recovery of the polarity of renal tubular epithelial cells

and protected the renal tubules. The therapeutic effects of SHT were superior to those of astragaloside as a single agent.

Abstract

To observe the effect of Compound Shenhua Tablet (复方肾华片

model group

astragaloside group [150 mg/(kg·d)]

SHT low-dose group [1.5 g/(kg·d)] and SHT high-dose group [3.0 g/(kg·d)]

with 10 rats in each group. After 1 week of continuous intragastric drug administration

surgery was performed to establish the model. At either 24 or 72 h after the surgery

5 rats in each group were sacrificed

blood biochemistry

renal pathology

immunoblot and immunohistochemical examinations were performed

and double immunofluorescence staining was observed under a laser confocal microscope. Compared with the sham surgery group

the serum creatinine (SCr) and blood urea nitrogen (BUN) levels were significantly increased

Na+-K+-ATPase protein level was decreased

and kidney injury molecule-1 (KIM-1) protein level was increased in the model group after the surgery (P<0.01 or P<0.05). Compared with the model group

the SCr

BUN

pathological scores

Na+-K+-ATPase

and the KIM-1 protein level of the three treatment groups were significantly improved at 72 h after the surgery (P<0.05 or P<0.01). And the SCr

BUN of the SHT low- and high-dose groups

promoted the recovery of the polarity of renal tubular epithelial cells

and protected the renal tubules. The therapeutic effects of SHT were superior to those of astragaloside as a single agent.

关键词

Compound Shenhua TabletAstragalosiderenal ischemic reperfusion injuryNa+-K+- adenosinetriphosphatase

Keywords

Compound Shenhua TabletAstragalosiderenal ischemic reperfusion injuryNa+-K+- adenosinetriphosphatase

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Shanghai Longhua Hospital, Affiliated to Shanghai University of TCM

Xiyuan Hospital, China Academy of Traditional Chinese Medicine

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Research Institute of Cardiovascular Diseases, The First Affiliated Hospital of Nanjing Medical University

Institute of Cardiovascular Diseases, The First Affiliated Hospital, Nanjing Medical University

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