Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats

Jia Guo; Tao Jin; Zi-qi Lin; Xiao-xiang Wang; Xiao-nan Yang; Qing Xia; Ping Xue

doi:10.1007/s11655-014-1750-5

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Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats

Updated：2021-08-27

- Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats
- Chinese Journal of Integrative Medicine Vol. 21, Issue 1, Pages: 29-35(2015)
- Affiliations：
  
  Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University,Chengdu,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072910), and the Science and Technology Supported Program of Sichuan Province, China (No. 2010SZ0068 and 2011SZ0291)
- DOI：10.1007/s11655-014-1750-5
  CLC：
- Published：2015，
  
  Published Online：10 May 2014，
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Guo, J., Jin, T., Lin, Zq. et al. Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats., Chin. J. Integr. Med. 21, 29–35 (2015). https://doi.org/10.1007/s11655-014-1750-5

Jia Guo, Tao Jin, Zi-qi Lin, et al. Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats. [J]. Chinese Journal of Integrative Medicine 21(1):29-35(2015)
Guo, J., Jin, T., Lin, Zq. et al. Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats., Chin. J. Integr. Med. 21, 29–35 (2015). https://doi.org/10.1007/s11655-014-1750-5 DOI：

Jia Guo, Tao Jin, Zi-qi Lin, et al. Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats. [J]. Chinese Journal of Integrative Medicine 21(1):29-35(2015) DOI： 10.1007/s11655-014-1750-5.

摘要

To investigate the effect of Chaiqin Chengqi Decoction (柴芩承气汤

CQCQD) on cholecystokinin receptor 1 (CCKR1)-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancreatitis (ANP). Twenty-seven Sprague-Dawley rats were randomized into three groups: the control group

the ANP group

and the CQCQD group (9 in each group). ANP rats were induced by two intraperitoneal injections of 8% L-arginine (pH=7.0

4.4 g/kg) over a 2-h period. Rats were treated with 1.5 mL/100 g body weight of CQCQD (CQCQD group) or physiological saline (control and ANP groups) at 2 h interval. And 6 h after induction

pancreatic tissues were collected for histopathological examination. Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression

phospholipase C (PLC) and inositol-1

5-triphosphate (IP3)

and determination of fluorescence intensity (FI) as a measure of intracellular calcium ion concentration [Ca2+]i. The pancreatic histopathological score (6.2±1.1) and the levels of PLC (1

187.2±228.2 μg/mL) and IP3 (872.2±88.4 μg/mL) of acinar cells in the ANP group were higher than those in the control (2.8±0.4

682.5±121.8 μg/mL

518.4±115.8 μg/mL) and the CQCQD (3.8±0.8

905.3±78.5 μg/mL

611.0±42.5 μg/mL) groups (P<0.05). [Ca2+]i FI for the ANP group (34.8±27.0) was higher than that in the control (5.1±2.2) and CQCQD (12.6±2.5) groups (P<0.05). The expression of pancreatic acinar cell CCKR1 mRNA in the ANP group was up-regulated (expression ratio=1.761; P=0.024) compared with the control group. The expression of pancreatic acinar cell CCKR1 mRNA in the CQCQD group was down-regulated (expression ratio=0.311; P=0.035) compared with the ANP group. The ratio of gray values of the CCKR1 and β-actin in the ANP group (1.43±0.17) was higher than those in the control (0.70±0.15) and CQCQD (0.79±0.11) groups (P<0.05). Pancreatic acinar cell calcium overload of ANP induced by L-arginine was related to the up-regulated expressions of pancreatic acinar cell CCKR1 mRNA and protein. CQCQD can down-regulate expressions of pancreatic acinar cell CCKR1 mRNA and protein to reduce the PLC and IP3 of pancreatic acinar cells

relieving the calcium overload and reducing the pathological changes in rats with ANP.

Abstract

To investigate the effect of Chaiqin Chengqi Decoction (柴芩承气汤

the ANP group

and the CQCQD group (9 in each group). ANP rats were induced by two intraperitoneal injections of 8% L-arginine (pH=7.0

4.4 g/kg) over a 2-h period. Rats were treated with 1.5 mL/100 g body weight of CQCQD (CQCQD group) or physiological saline (control and ANP groups) at 2 h interval. And 6 h after induction

pancreatic tissues were collected for histopathological examination. Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression

phospholipase C (PLC) and inositol-1

5-triphosphate (IP3)

and determination of fluorescence intensity (FI) as a measure of intracellular calcium ion concentration [Ca2+]i. The pancreatic histopathological score (6.2±1.1) and the levels of PLC (1

187.2±228.2 μg/mL) and IP3 (872.2±88.4 μg/mL) of acinar cells in the ANP group were higher than those in the control (2.8±0.4

682.5±121.8 μg/mL

518.4±115.8 μg/mL) and the CQCQD (3.8±0.8

905.3±78.5 μg/mL

relieving the calcium overload and reducing the pathological changes in rats with ANP.

关键词

Acute PancreatitisChaiqin Chengqi Decoctioncholecystokinin receptor 1Chinese Medicine

Keywords

Acute PancreatitisChaiqin Chengqi Decoctioncholecystokinin receptor 1Chinese Medicine

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