Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats

Lu-ping Tang; Wei Xiao; Yi-fang Li; Hai-bo Li; Zhen-zhong Wang; Xin-sheng Yao; Hiroshi Kurihara; Rong-rong He

doi:10.1007/s11655-014-1758-x

Your Location：

Home >

Browse articles >

Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats

Updated：2021-08-27

- Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats
- Chinese Journal of Integrative Medicine Vol. 20, Issue 8, Pages: 591-599(2014)
- Affiliations：
  
  1. Pharmacy College, Jinan University,Guangzhou,China
  2. State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process,Jiangsu Province,Lianyungang,China
- Author bio：
- Funds：
  
  Supported by Distinguished Trans-Century Training Program Foundation for the Talents of the State Education Commission, China (No. NCET-12-0678), Natural Science Foundation of Guangdong Province, China (No. S20120011316), Science and Technology Program of Guangzhou, China (No. 2012J22000073), Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (No. LYM11023)
- DOI：10.1007/s11655-014-1758-x
  CLC：
- Published：2014，
  
  Published Online：10 June 2014，
Scan for full text
Tang, Lp., Xiao, W., Li, Yf. et al. Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats., Chin. J. Integr. Med. 20, 591–599 (2014). https://doi.org/10.1007/s11655-014-1758-x

Lu-ping Tang, Wei Xiao, Yi-fang Li, et al. Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats. [J]. Chinese Journal of Integrative Medicine 20(8):591-599(2014)
Tang, Lp., Xiao, W., Li, Yf. et al. Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats., Chin. J. Integr. Med. 20, 591–599 (2014). https://doi.org/10.1007/s11655-014-1758-x DOI：

Lu-ping Tang, Wei Xiao, Yi-fang Li, et al. Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats. [J]. Chinese Journal of Integrative Medicine 20(8):591-599(2014) DOI： 10.1007/s11655-014-1758-x.

摘要

To evaluate the protective effects of Reduning Injection (热毒宁注射液

RDN)

a patent Chinese medicine

on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats and its underlying mechanisms of action. Sixty male Sprague-Dawley rats were randomly divided into 6 groups

including normal control

model

dexamethasone (DEX

5 mg/kg)

RDN-H (720 mg/kg)

RDN-M (360 mg/kg) and RDN-L (180 mg/kg) groups

with 10 rats in each group. Rats were challenged with intravenous injection of LPS 1 h after intraperitoneal treatment with RDN or DEX. At 6 h after LPS challenge

lung tissues and bronchoalveolar lavage fluid (BALF) were collected

and the number of inflammatory cells was determined. The right lungs were collected for histopathologic examination

measurement of gene and protein expressions

superoxide dismutase (SOD) and myeloperoxidase (MPO) activities. In vivo pretreatment of RDN (360

720 mg/kg) significantly reduced the weight of wet to dry (W/D) ratio of lung

protein content in BALF

and led to remarkable attenuation of LPS-induced histopathological changes in the lungs. Meanwhile

RDN enormously decreased BALF total inflammatory cells

especially neutrophil and macrophage cell numbers. Moreover

RDN increased SOD activity

inhibited MPO activity

alleviated LPS-induced tumor neurosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) expression in lung tissues. Furthermore

RDN (720 mg/kg) efficiently weakened nuclear factorkappa B (NF-κB) gene and protein expression. Anti-inflammatory effects of RDN was demonstrated to be preventing pulmonary neutrophil infiltration

lowering MPO activity

TNF-α and iNOS gene expression by inhibiting NF-κB activity in LPS-induced ALI.

Abstract

To evaluate the protective effects of Reduning Injection (热毒宁注射液

RDN)

a patent Chinese medicine

on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats and its underlying mechanisms of action. Sixty male Sprague-Dawley rats were randomly divided into 6 groups

including normal control

model

dexamethasone (DEX

5 mg/kg)

RDN-H (720 mg/kg)

RDN-M (360 mg/kg) and RDN-L (180 mg/kg) groups

with 10 rats in each group. Rats were challenged with intravenous injection of LPS 1 h after intraperitoneal treatment with RDN or DEX. At 6 h after LPS challenge

lung tissues and bronchoalveolar lavage fluid (BALF) were collected

and the number of inflammatory cells was determined. The right lungs were collected for histopathologic examination

measurement of gene and protein expressions

superoxide dismutase (SOD) and myeloperoxidase (MPO) activities. In vivo pretreatment of RDN (360

720 mg/kg) significantly reduced the weight of wet to dry (W/D) ratio of lung

protein content in BALF

and led to remarkable attenuation of LPS-induced histopathological changes in the lungs. Meanwhile

RDN enormously decreased BALF total inflammatory cells

especially neutrophil and macrophage cell numbers. Moreover

RDN increased SOD activity

inhibited MPO activity

alleviated LPS-induced tumor neurosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) expression in lung tissues. Furthermore

RDN (720 mg/kg) efficiently weakened nuclear factorkappa B (NF-κB) gene and protein expression. Anti-inflammatory effects of RDN was demonstrated to be preventing pulmonary neutrophil infiltration

lowering MPO activity

TNF-α and iNOS gene expression by inhibiting NF-κB activity in LPS-induced ALI.

关键词

Acute Lung Injurylipopolysaccharideneutrophilschemokinenuclear factor-kappa BReduning InjectionChinese Patent Medicine

Keywords

Acute Lung Injurylipopolysaccharideneutrophilschemokinenuclear factor-kappa BReduning InjectionChinese Patent Medicine

references

Mei SHJ, McCarter SD, Deng Y, Parker CH, Liles WC, Stewart DJ. Prevention of LPS-induced acute lung injury in mice by mesenchymal stem cells overexpressing angiopoietin-1. PLoS Med 2007;4:1525–1537.

Moon C, Han JR, Park HJ, Hah JS, Kang JL. Synthetic RGDS peptide attenuates lipopolysaccharide-induced pulmonary inflammation by inhibiting integrin signaled MAP kinase pathways. Respir Res 2009;10:18–23.

Johnston CJ, Finkelstein JN, Gelein R, Oberdorster G. Pulmonary cytokine and chemokine mRNA levels after inhalation of lipopolysaccharide in C57BL/6 mice. Toxicol Sci 1998;46:300–307.

Beutler B, Rietschel ET. Innate immune sensing and its roots: the story of endotoxin. Nat Rev Immunol 2003;3:169–176.

Tschernig T, Janardhan KS, Pabst R, Singh B. Lipopolysaccharide-induced inflammation in the perivascular space in lungs. J Occup Med Toxicol 2008;30:13–17.

Itoh T, Obata H, Murakami S, Hamada K, Kangawa K, Kimura H, et al. Adrenomedullin ameliorates lipopolysaccharideinduced acute lung injury in rats. Am J Physiol Lung Cell Mol Physiol 2007;293:L446–L452.

Liu SH, Xu XR, Ma K, Xu B. Protection of carbon monoxide inhalation on lipopolysaccharide-induced multiple organ injury in rats. Chin Med Sci J 2007;22:169–176.

Nonas S, Miller I, Kawkitinarong K, Chatchavalvanich S, Gorshkova I, Bochkov VN, et al. Oxidized phospholipids reduce vascular leak and inflammation in rat model of acute lung injury. Am J Respir Crit Care Med 2006;173:1130–1138.

Shimizu M, Hasegawa N, Nishimura T, Endo Y, Shiraishi Y, Yamasawa W, et al. Effects of TNF-alpha-converting enzyme inhibition on acute lung injury induced by endotoxin in the rat. Shock 2009;32:535–540.

Mafra de LF, Naves KT, Machado AH, Albertini R, Villaverde AB, Aimbire F. Lung inflammation and endothelial cell damage are decreased after treatment with phototherapy (PhT) in a model of acute lung injury induced by Escherichia coli lipopolysaccharide in the rat. Cell Biol Int 2009;33:1212–1221.

Brass DM, Hollingsworth JW, Cinque M, Li Z, Potyts E, Toloza E, et al. Chronic LPS inhalation causes emphysemalike changes in mouse lung that are associated with apoptosis. Am J Respir Cell Mol Biol 2008;39:584–590.

Uchiyama K, Takano H, Yanagisawa R, Inoue K, Naito Y, Yoshida N, et al. A novel water-soluble vitamin E derivative prevents acute lung injury by bacterial endotoxin. Clin Exp Pharm Physiol 2004;31:226–230.

Feng NH, Chu SJ, Wang D, Hsu K, Lin CH, Lin HI. Effects of various antioxidants on endotoxin-induced lung injury and gene expression: mRNA expressions of MnSOD, interleukin-1β and iNOS. Chin J Physiol 2004;47:111–120.

Gao J, Zeng B, Zhou L. The protective effects of early treatment with propofol on endotoxin-induced acute lung injury in rats. Middle East J Anesthesiol 2003;17:379–401.

Blackwell TS, Christman JW. The role of nuclear factor- κB in cytokine gene regulation. Am J Respir Cell Mol Biol 1997;17:3–9.

Bhatia M, Moochhala S. Role of inflammatory mediators in the pathophysiology of acute respiratory distress syndrome. J Pathol 2004;202:145–156.

Santos VS, Silva BF, Aguiar LA, Romana SB, Marinho MC, Brando LA. Oxidative stress in mouse plasma and lungs induced by cigarette smoke and lipopolysaccharide. Environ Res 2008;108:199–204.

Goraca A, Josefowicz-Okonkwo G. Protective effects of early treatment with lipoicacid in LPS-induced lung injury in rats. J Physiol Pharm 2007;58:541–549.

Liu SH, Xu XR, Ma K, Xu B. Protection of carbon monoxide inhalation on lipopolysaccharide-induced multiple organ injury in rats. Chin Med Sci J 2007;22:169–176.

Chen J, Liu XJ, Shuo QL, Li SQ, Luo FM. Ghrelin attenuates lipopolysaccharide-induced acute lung injury through NO pathway. Med Sci Monit 2008;14:141–146.

Ling CK. Evaluation on effect of Reduning Injection on aging pneumonia based on the method of randomized control trial. Guide China Med (Chin) 2010;8:453–454.

Mo XQ. Therapeutic effect and security observation of single use of Reduning on treating child acute upper respiratory infection. Guide China Med (Chin) 2010;8:141–143.

Yu J. A new drug of anti-bacterium and anti-virus—Reduning Injection. Guide China Med (Chin) 2010;8:1672–1681.

Tumurkhuu G, Koide N, Dagvadorj J, Morikawa A, Hassan F, Islam S, et al. The mechanism of development of acute lung injury in lethal endotoxic shock using alpha-galactosylceramide sensitization. Clin Exp Immunol 2008;152:182–191.

Tsuruta Y, Park YJ, Siegal GP, Liu G, Abraham E. Involvement of vitronectin in lipopolysaccaride-induced acute lung injury. J Immunol 2007;179:7079–7086.

Beck BD, Brain JD, Bohannon DE. An in vivo hamster bioassay to assess the toxicity of particulates for the lungs. Toxicol Appl Pharmacol 1982;66:19–29.

Villarino M, Sandin-Espana P, Melgarejo P, De Cal A. High chlorogenic and neochlorogenic acid levels in immature peaches reduce Monilinia laxa infection by interfering with fungal melanin biosynthesis. J Agric Food Chem 2011;59:3205–3213.

dos Santos MD, Almeida MC, Lopes NP, de Souza GEP. Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol Pharm Bull 2006;29:2236–2240.

Xia DZ, Shi JY, Gong JY, Wu XQ, Yang Q, Zhang Y. Antioxidant activity of Chinese mei (Prunus mume) and its active phytochemicals. J Med Plan Res 2010;4:1156–1160.

Yang XF, Cai QR, He JP, Chu X, Wei MM, Feng XR, et al. Geniposide, an iridoid glucoside derived from Gardenia jasminoides, protects against lipopolysaccharide-induced acute lung injury in mice. Plant Med 2012;6:557–564.

Zhang G, He JL, Xie XY, Yu C. LPS-induced iNOS expression in N9 microglial cells is suppressed by geniposide via ERK, p38 and nuclear factor-κB signaling pathways. Int J Mol Med 2012;30:561–568.

Cepkova M, Matthay MA. Pharmacotherapy of acute lung injury and the acute respiratory distress syndrome. J Intensive Care Med 2006;21:119–143.

Klebanoff SJ. Myeloperoxidase: friend and foe. J Leuk Biol 2005;77:598–625.

Winterbourn CC, Vissers MCM, Kettle AJ. Myeloperoxidase. Curr Opin Hematol 2000;7:53–58.

Staub NC, Longworth KE, Serikov V, Jerome EH, Elsasser T. Detergent inhibits 70–90% of responses to intravenous endotoxin in awake sheep. J Appl Physiol 2001;90:1788–1797.

Sone Y, Serikov VB, Staub Sr NC. Intravascular macrophage depletion attenuates endotoxin lung injury in anesthetized sheep. J Appl Physiol 1999;87:1354–1359.

Giebelen IAJ, van Westerloo DJ, LaRosa GJ, de Vos AF, van der Poll T. Local stimulation of alpha7 cholinergic receptors inhibits LPS-induced TNF-alpha release in the mouse lung. Shock 2007;28:700–703.

Moraes TJ, Zurawska, JH, Downey GP. Neutrophil granule contents in the pathogenesis of lung injury. Curr Opin Hematol 2006;13:21–27.

Roth E, Manhart N, Wessner B. Assessing the antioxidative status in critically ill patients. Curr Opin Clin Nutrit Metab Care 2004;7:161–168.

Wang LF, Patel M, Razavi HM, Weicker S, Joseph MG, McCormack DG, et al. Role of inducible nitric oxide synthase in pulmonary microvascular protein leak in murine sepsis. Am J Respir Crit Care Med 2002;165:1634–1639.

Wang LF, Mehta S, Weicker S, Scott JA, Joseph M, Razavi HM, et al. Relative contribution of hemopoietic and pulmonary parenchymal cells to lung inducible nitric oxide synthase (iNOS) activity in murine endotoxemia. Biochem Biophys Res Commun 2001;283:694–699.

Fan J, Ye RD, Malik AB. Transcriptional mechanisms of acute lung injury. Am J Physiol Lung Cell Mol Physiol 2001;281:L1037–L1050.

Baeuerle PA, Baltimore D. NF-kappa B: ten years after. Cell 1996;87:13–20.

Takeuchi O, Akira S. Pattern recognition receptors and inflammation. Cell 2010;140:805–820.

FULL TEXT LINK

More>

Views

984

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways

Effect of safflor yellow injection on inhibiting lipopolysaccharide-induced pulmonary inflammatory injury in mice

Effect of puerarin on the release of interleukin-8 in co-culture of human bronchial epithelial cells and neutrophils

Effect of San’ao Decoction (三拗汤) on the airway inflammation and hyperresponsiveness in a murine model of lipopolysaccharide-enhanced asthma

Salvianolic acid B inhibits the TLR4-NFκB-TNFα pathway and attenuates neonatal rat cardiomyocyte injury induced by lipopolysaccharide

Related Author

No data

Related Institution

Deparment of Laboratory Medicine, Wenzhou Medical College

Department of Clinical Laboratory, Chinese People’s Liberation Army General Hospital

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Ministry of Education), Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College

Department of Pharmacology, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University

Department of Clinical Laboratory Medicine, Affiliate Hospital of Logistical College of Chinese People’s Armed Police Forces

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰