Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.

Chinthamony Arul Raj; Paramasivam Ragavendran; Dominic Sophia; Thangarajan Starlin; Muthian Ahalliya Rathi; Velliyur Kanniappan Gopalakrishnan

doi:10.1007/s11655-014-1762-1

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Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.

Updated：2021-08-27

- Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.
- Chinese Journal of Integrative Medicine Vol. 22, Issue 9, Pages: 691-695(2016)
- Affiliations：
  
  1. Department of Biochemistry, Karpagam University, Coimbatore, Tamil Nadu,India
  2. Department of Bioinformatics, Karpagam University, Coimbatore, Tamil Nadu,India
  3. Department of Biochemistry, Sree Narayana Guru College of Arts and Science, KG. Chavadi, Coimbator, Tamil Nadu,India
- Author bio：
- Funds：
- DOI：10.1007/s11655-014-1762-1
  CLC：
- Published：2016，
  
  Published Online：20 August 2014，
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Raj, C.A., Ragavendran, P., Sophia, D. et al. Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.., Chin. J. Integr. Med. 22, 691–695 (2016). https://doi.org/10.1007/s11655-014-1762-1

Chinthamony Arul Raj, Paramasivam Ragavendran, Dominic Sophia, et al. Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.. [J]. Chinese Journal of Integrative Medicine 22(9):691-695(2016)
Raj, C.A., Ragavendran, P., Sophia, D. et al. Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.., Chin. J. Integr. Med. 22, 691–695 (2016). https://doi.org/10.1007/s11655-014-1762-1 DOI：

Chinthamony Arul Raj, Paramasivam Ragavendran, Dominic Sophia, et al. Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum.. [J]. Chinese Journal of Integrative Medicine 22(9):691-695(2016) DOI： 10.1007/s11655-014-1762-1.

摘要

To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10

000×g at 4°C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD)

catalase (CAT)

glutathione peroxidase (GPx)

glutathione S-transferase (GST)

ascorbate oxidase

peroxidase

polyphenol oxidase (PPO) and non-enzymatic antioxidants such as vitamin C

total reduced glutathione (TRG) and lipid peroxidation (LPO). The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein

GST 372.11±5.70 μmol of 1-chloro 2

4 dinitrobenzene (CDNB)-reduced glutathione (GSH) conjugate formed/min/mg protein

GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein

peroxidases 173.12±9.40 μmol/g tissue

TRG 75.27±3.55 μg/mg protein

SOD 58.03±2.11 U/mg protein

CAT 46.70±2.35 μmol of H2O2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue

LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 μmol/g tissue in moderate amount. Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future

Alpinia purpurata may serve as a good pharmacotherapeutic agent.

Abstract

To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10

000×g at 4°C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD)

catalase (CAT)

glutathione peroxidase (GPx)

glutathione S-transferase (GST)

ascorbate oxidase

peroxidase

polyphenol oxidase (PPO) and non-enzymatic antioxidants such as vitamin C

total reduced glutathione (TRG) and lipid peroxidation (LPO). The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein

GST 372.11±5.70 μmol of 1-chloro 2

4 dinitrobenzene (CDNB)-reduced glutathione (GSH) conjugate formed/min/mg protein

GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein

peroxidases 173.12±9.40 μmol/g tissue

TRG 75.27±3.55 μg/mg protein

SOD 58.03±2.11 U/mg protein

CAT 46.70±2.35 μmol of H2O2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue

Alpinia purpurata may serve as a good pharmacotherapeutic agent.

关键词

Alpinia purpurataenzymatic antioxidantsnon-enzymatic antioxidantsoxidative stressfree radicals

Keywords

Alpinia purpurataenzymatic antioxidantsnon-enzymatic antioxidantsoxidative stressfree radicals

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