Effect of p27 gene combined with Pientzehuang (片仔癀) on tumor growth in osteosarcoma-bearing nude mice

Shou-song Ren; Fang Yuan; Ying-hong Liu; Le-tian Zhou; Jun Li

doi:10.1007/s11655-014-1766-x

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Effect of p27 gene combined with Pientzehuang (片仔癀) on tumor growth in osteosarcoma-bearing nude mice

Updated：2021-08-27

- Effect of p27 gene combined with Pientzehuang (片仔癀) on tumor growth in osteosarcoma-bearing nude mice
- Chinese Journal of Integrative Medicine Vol. 21, Issue 11, Pages: 830-836(2015)
- Affiliations：
  
  1. Cheeloo Health Science Center of Shandong University,Jinan,China
  2. Department of Spine Surgery, Jimo City People’s Hospital,Shandong Province,Qingdao,China
  3. Department of Nephrology, the Second Xiangya Hospital, Central South University,Changsha,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-014-1766-x
  CLC：
- Published：2015，
  
  Published Online：20 August 2014，
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Ren, Ss., Yuan, F., Liu, Yh. et al. Effect of p27 gene combined with Pientzehuang (片仔癀) on tumor growth in osteosarcoma-bearing nude mice., Chin. J. Integr. Med. 21, 830–836 (2015). https://doi.org/10.1007/s11655-014-1766-x

Shou-song Ren, Fang Yuan, Ying-hong Liu, et al. Effect of p27 gene combined with Pientzehuang (片仔癀) on tumor growth in osteosarcoma-bearing nude mice. [J]. Chinese Journal of Integrative Medicine 21(11):830-836(2015)
Ren, Ss., Yuan, F., Liu, Yh. et al. Effect of p27 gene combined with Pientzehuang (片仔癀) on tumor growth in osteosarcoma-bearing nude mice., Chin. J. Integr. Med. 21, 830–836 (2015). https://doi.org/10.1007/s11655-014-1766-x DOI：

Shou-song Ren, Fang Yuan, Ying-hong Liu, et al. Effect of p27 gene combined with Pientzehuang (片仔癀) on tumor growth in osteosarcoma-bearing nude mice. [J]. Chinese Journal of Integrative Medicine 21(11):830-836(2015) DOI： 10.1007/s11655-014-1766-x.

摘要

To observe the effect of p27 gene recombinant adenovirus combined with Chinese medicine Pientzehuang (片仔癀) on the growth of xenografted human osteosarcoma in nude mice. Tissue transplantation was used to construct the orthotopic model of human osteosarcoma Saos-2 cell in nude mice. Thirty tumor-bearing nude mice were randomly divided into 5 groups with 6 mice in each group: blank control group (model of osteosarcoma)

empty vector group (recombinant adeno-associated virus-multiple cloning site)

Pientzehuang group

p27 gene group and combined treatment group (p27 gene combined with Pientzehuang). The effect of combined treatment on human osteosarcoma was analyzed through the tumor formation

tumor volume and inhibition rate of tumor growth. The expression of p27 was measured by immunohistochemical staining and Western blot. The orthotopic model of osteosarcoma in nude mice was successfully constructed. The general appearance of tumor-bearing nude mice in Pientzehuang and p27 gene groups was markedly improved compared with the blank control group; and in the combined treatment group it was signifificantly improved compared with the Pientzehuang and p27 gene groups. The tumor growth in the Pientzehuang and p27 gene groups was significantly inhibited compared with the blank control group P<0.05); while in the combined treatment group it was markedly inhibited compared with the Pientzehuang and p27 gene groups (P<0.05). The rates of tumor growth inhibition were 34.1%

56.5% and 63.8% in the Pientzehuang

p27 gene and combined treatment groups

respectively. Meanwhile

the protein expression of p27 gene in the p27 gene group was signifificantly increased compared with the blank control group (P<0.05); and it was signifificantly increased in the combined treatment group compared with the p27 gene and Pientzehuang groups (P<0.05). p27 gene introduced by adenovirus combined with Pientzehuang can inhibit the growth of human osteosarcoma cell Saos-2 in nude mice.

Abstract

empty vector group (recombinant adeno-associated virus-multiple cloning site)

Pientzehuang group

p27 gene group and combined treatment group (p27 gene combined with Pientzehuang). The effect of combined treatment on human osteosarcoma was analyzed through the tumor formation

56.5% and 63.8% in the Pientzehuang

p27 gene and combined treatment groups

respectively. Meanwhile

关键词

Pientzehuangp27 genehuman osteosarcomatumor growthChinese Medicine

Keywords

Pientzehuangp27 genehuman osteosarcomatumor growthChinese Medicine

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