Expression of T-helper 17 cells and signal transducers in patients with psoriasis vulgaris of blood-heat syndrome and blood-stasis syndrome

Bin Fan; Xin Li; Kan Ze; Rong Xu; Ruo-fei Shi; Lin Geng; Fu-lun Li; Yi-fei Wang; Jie Chen; Bin Li

doi:10.1007/s11655-014-1769-7

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Expression of T-helper 17 cells and signal transducers in patients with psoriasis vulgaris of blood-heat syndrome and blood-stasis syndrome

Updated：2021-08-27

- Expression of T-helper 17 cells and signal transducers in patients with psoriasis vulgaris of blood-heat syndrome and blood-stasis syndrome
- Chinese Journal of Integrative Medicine Vol. 21, Issue 1, Pages: 10-16(2015)
- Affiliations：
  
  1. Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China
  2. Department of Dermatology, Ruijin Hospital, Affiliated to Shanghai Jiaotong University School of Medicine,Shanghai,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81302971, 81273764), and Innovative Research Team in University of Shanghai Municipal Education Commission, China (Phase II)
- DOI：10.1007/s11655-014-1769-7
  CLC：
- Published：2015，
  
  Published Online：23 September 2014，
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Fan, B., Li, X., Ze, K. et al. Expression of T-helper 17 cells and signal transducers in patients with psoriasis vulgaris of blood-heat syndrome and blood-stasis syndrome., Chin. J. Integr. Med. 21, 10–16 (2015). https://doi.org/10.1007/s11655-014-1769-7

Bin Fan, Xin Li, Kan Ze, et al. Expression of T-helper 17 cells and signal transducers in patients with psoriasis vulgaris of blood-heat syndrome and blood-stasis syndrome. [J]. Chinese Journal of Integrative Medicine 21(1):10-16(2015)
Fan, B., Li, X., Ze, K. et al. Expression of T-helper 17 cells and signal transducers in patients with psoriasis vulgaris of blood-heat syndrome and blood-stasis syndrome., Chin. J. Integr. Med. 21, 10–16 (2015). https://doi.org/10.1007/s11655-014-1769-7 DOI：

Bin Fan, Xin Li, Kan Ze, et al. Expression of T-helper 17 cells and signal transducers in patients with psoriasis vulgaris of blood-heat syndrome and blood-stasis syndrome. [J]. Chinese Journal of Integrative Medicine 21(1):10-16(2015) DOI： 10.1007/s11655-014-1769-7.

摘要

To investigate the levels of cytokines related to T-helper (Th) 17 cells in serum and signal transducers in the psoriatic lesions of patients with psoriasis vulgaris of blood-heat syndrome (BHS) and blood-stasis syndrome (BSS). Sixty patients with psoriasis vulgaris were divided into the BHS and BSS groups according to the syndrome differentiation of Chinese medicine (CM). Ten healthy subjects were considered as the control group. Cytokine levels of interleukin (IL)-17

IL-23 and IL-6 in serum were determined by enzyme-linked immunosorbent assay. Expression levels of signal transducer and activator of transcription 3 (STAT3)

p38-mitogen-activated protein kinase (MAPK) and STAT6 in the psoriatic lesions were determined using immunohistochemistry (IHC)

Western blot

and real-time quantitative reverse transcription polymerase chain reaction

respectively. Production of IL-17

IL-23 and IL-6 in the BHS group and BSS group were significantly increased compared with those in the control group (P<0.05). Levels of IL-17 and IL-23 in the BHS group were higher than those in the BSS group (P<0.05). Compared with the control group

IHC positive expressions and protein expressions of STAT3 and p38-MAPK

and the STAT3 mRNA expressions in the BHS and BSS groups were significantly higher (P<0.05 or P<0.01). The protein expression of STAT3 in the BHS group was significantly higher than that in the BSS group (P<0.05). Cytokines in serum and signal transducers in the psoriatic lesions alter with various CM syndromes of psoriasis. The results provide scientific basis for the treatment based on syndrome differentiation of CM in treating psoriasis vulgaris.

Abstract

IL-23 and IL-6 in serum were determined by enzyme-linked immunosorbent assay. Expression levels of signal transducer and activator of transcription 3 (STAT3)

p38-mitogen-activated protein kinase (MAPK) and STAT6 in the psoriatic lesions were determined using immunohistochemistry (IHC)

Western blot

and real-time quantitative reverse transcription polymerase chain reaction

respectively. Production of IL-17

IHC positive expressions and protein expressions of STAT3 and p38-MAPK

关键词

psoriasisT-helper 17 cellssignal transducersblood-heat syndromeblood-stasis syndromeChinese Medicine

Keywords

psoriasisT-helper 17 cellssignal transducersblood-heat syndromeblood-stasis syndromeChinese Medicine

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