Apelin-APJ effects of ginsenoside-Rb1 depending on hypoxia-induced factor 1α in hypoxia neonatal cardiomyocytes

Hong-liang Kong; Zhan-quan Li; Shu-mei Zhao; Long Yuan; Zhi-lin Miao; Ying Liu; Ru-ming Guan

doi:10.1007/s11655-014-1774-2

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Apelin-APJ effects of ginsenoside-Rb1 depending on hypoxia-induced factor 1α in hypoxia neonatal cardiomyocytes

Updated：2021-08-27

- Apelin-APJ effects of ginsenoside-Rb1 depending on hypoxia-induced factor 1α in hypoxia neonatal cardiomyocytes
- Chinese Journal of Integrative Medicine Vol. 21, Issue 2, Pages: 139-146(2015)
- Affiliations：
  
  1. Cardiology Center, The People’s Hospital of Liaoning Province,Shenyang,China
  2. Shenyang Normal University,Shenyang,China
- Author bio：
- Funds：
  
  Supported by Liaoning Province Science and Technique Foundation of China (No. 201102107)
- DOI：10.1007/s11655-014-1774-2
  CLC：
- Published：2015，
  
  Published Online：3 June 2014，
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Kong, Hl., Li, Zq., Zhao, Sm. et al. Apelin-APJ effects of ginsenoside-Rb1 depending on hypoxia-induced factor 1α in hypoxia neonatal cardiomyocytes., Chin. J. Integr. Med. 21, 139–146 (2015). https://doi.org/10.1007/s11655-014-1774-2

Hong-liang Kong, Zhan-quan Li, Shu-mei Zhao, et al. Apelin-APJ effects of ginsenoside-Rb1 depending on hypoxia-induced factor 1α in hypoxia neonatal cardiomyocytes. [J]. Chinese Journal of Integrative Medicine 21(2):139-146(2015)
Kong, Hl., Li, Zq., Zhao, Sm. et al. Apelin-APJ effects of ginsenoside-Rb1 depending on hypoxia-induced factor 1α in hypoxia neonatal cardiomyocytes., Chin. J. Integr. Med. 21, 139–146 (2015). https://doi.org/10.1007/s11655-014-1774-2 DOI：

Hong-liang Kong, Zhan-quan Li, Shu-mei Zhao, et al. Apelin-APJ effects of ginsenoside-Rb1 depending on hypoxia-induced factor 1α in hypoxia neonatal cardiomyocytes. [J]. Chinese Journal of Integrative Medicine 21(2):139-146(2015) DOI： 10.1007/s11655-014-1774-2.

摘要

To investigate whether ginsenoside-Rb1 (Gs-Rb1) inhibits the apoptosis of hypoxia cardiomyocytes by up-regulating apelin-APJ system and whether the system is affected by hypoxia-induced factor 1α (Hif-1α). Neonatal rat cardiomyocytes were randomly divided into 6 groups: a control group

a simple CoCl group

a simple Gs-Rb1 group

a CoCl and Gs-Rb1 hypoxia group

a CoCl and 3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1) group

a CoCl and YC-1 group and a Gs-Rb1 group

in which YC-1 inhibits the synthesis and accelerates the degradation of Hif-1a. The concentration of CoCl

Gs-Rb1 and YC-1 was 500 μmol/L

200 μmol/L and 5 μmol/L

respectively; the apoptosis ratio was analyzed with a flow cytometer; and apelin

APJ and Hif-1α were assayed with immunocytochemistry

Western blot assays and reverse transcription polymerase chain reaction (RT-PCR). (1) The anti-apoptosis effect of Gs-Rb1 on hypoxia cardiomyocytes was significantly inhibited by YC-1; (2) Hypoxia significantly up-graded the expression of mRNA and protein of apelin; this effect was further reinforced by Gs-Rb1 and significantly inhibited by YC-1; (3) Gs-Rb1 further strengthened the expression of APJ mRNA and APJ proteins once hypoxia occurred

which was significantly inhibited by YC-1; (4) Gs-Rb1 significantly increased the expression of Hif-1α

which was completely abolished by YC-1; (5) There was a negative relationship between AR and apelin (or APJ

including mRNA and protein)

a positive correlation between apelin (or APJ) protein and Hif-1a protein

in hypoxia cardiomyocytes. The apelin-APJ system plays an important role in the anti-apoptosis effect of Gs-Rb1 on hypoxia neonatal cardiomyocytes

which was partly adjusted by Hif-1α.

Abstract

a simple CoCl group

a simple Gs-Rb1 group

a CoCl and Gs-Rb1 hypoxia group

a CoCl and 3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1) group

a CoCl and YC-1 group and a Gs-Rb1 group

in which YC-1 inhibits the synthesis and accelerates the degradation of Hif-1a. The concentration of CoCl

Gs-Rb1 and YC-1 was 500 μmol/L

200 μmol/L and 5 μmol/L

respectively; the apoptosis ratio was analyzed with a flow cytometer; and apelin

APJ and Hif-1α were assayed with immunocytochemistry

which was significantly inhibited by YC-1; (4) Gs-Rb1 significantly increased the expression of Hif-1α

which was completely abolished by YC-1; (5) There was a negative relationship between AR and apelin (or APJ

including mRNA and protein)

a positive correlation between apelin (or APJ) protein and Hif-1a protein

in hypoxia cardiomyocytes. The apelin-APJ system plays an important role in the anti-apoptosis effect of Gs-Rb1 on hypoxia neonatal cardiomyocytes

which was partly adjusted by Hif-1α.

关键词

ginsenosides-Rb1cardiomyocyteshypoxiaapelinAPJhypoxia-induced factor 1α

Keywords

ginsenosides-Rb1cardiomyocyteshypoxiaapelinAPJhypoxia-induced factor 1α

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