Effects of Biejia Ruangan Tablet (复方鳖甲软肝片)-containing serum on matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in cultured renal interstitial fibroblasts

Jin Zhou; Xiang-mei Chen; Shu-wen Liu; Bo Fu; Quan Hong; Shu-juan Wang

doi:10.1007/s11655-014-1784-0

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Effects of Biejia Ruangan Tablet (复方鳖甲软肝片)-containing serum on matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in cultured renal interstitial fibroblasts

Updated：2021-08-27

- Effects of Biejia Ruangan Tablet (复方鳖甲软肝片)-containing serum on matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in cultured renal interstitial fibroblasts
- Chinese Journal of Integrative Medicine Vol. 21, Issue 2, Pages: 152-156(2015)
- Affiliations：
  
  1. Department of Nephrology, the PLA General Hospital,Beijing,China
  2. Department of Medical Records, the PLA General Hospital,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Science Foundation of China (No. 30130220 and No. 30873345);National Natural Science Found for Innovative Research Groups Science Foundation of China (No. 30121005)
- DOI：10.1007/s11655-014-1784-0
  CLC：
- Published：2015，
  
  Published Online：16 December 2014，
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Zhou, J., Chen, Xm., Liu, Sw. et al. Effects of Biejia Ruangan Tablet (复方鳖甲软肝片)-containing serum on matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in cultured renal interstitial fibroblasts., Chin. J. Integr. Med. 21, 152–156 (2015). https://doi.org/10.1007/s11655-014-1784-0

Jin Zhou, Xiang-mei Chen, Shu-wen Liu, et al. Effects of Biejia Ruangan Tablet (复方鳖甲软肝片)-containing serum on matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in cultured renal interstitial fibroblasts. [J]. Chinese Journal of Integrative Medicine 21(2):152-156(2015)
Zhou, J., Chen, Xm., Liu, Sw. et al. Effects of Biejia Ruangan Tablet (复方鳖甲软肝片)-containing serum on matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in cultured renal interstitial fibroblasts., Chin. J. Integr. Med. 21, 152–156 (2015). https://doi.org/10.1007/s11655-014-1784-0 DOI：

Jin Zhou, Xiang-mei Chen, Shu-wen Liu, et al. Effects of Biejia Ruangan Tablet (复方鳖甲软肝片)-containing serum on matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in cultured renal interstitial fibroblasts. [J]. Chinese Journal of Integrative Medicine 21(2):152-156(2015) DOI： 10.1007/s11655-014-1784-0.

摘要

To investigate the effects of Biejia Ruangan Tablet (复方鳖甲软肝片方

BRT)-containing serum on the expression of matrix metalloproteinase (MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) in cultured renal interstitial fibroblasts. Different BRT-containing sera were prepared by gastric gavages to rats with the high-dose (7 g/kg)

mid-dose (3.5 g/kg)

and low-dose (1.75 g/kg) BRT respectively. The expression of extracellular matrix in NRK-49F cells was induced by treatment with human transforming growth factor-β1 (recombined human TGF-β1)

and BRT-containing serum. Western blotting and Northern blotting were used to measure type I and III procollagen

MMP-9

and TIMP-1. The high dose BRT-containing serum could decrease the type I and III procollagen gene expression which boosted by TGF-β1

at the same time cut down TIMP-1 protein and gene expression which increased by TGF-β1 (P <0.05). Treatment of cells with recombined human TGF-β1 had no significant effect on MMP-9 expression and BRT-containing serum also had no effect on MMP-9 expression. High dose BRT has anti-fibrosis effects in NRK-49F cells

as indicated by its inhibition of type I and III procollagen and TIMP-1 expression.

Abstract

To investigate the effects of Biejia Ruangan Tablet (复方鳖甲软肝片方

mid-dose (3.5 g/kg)

and low-dose (1.75 g/kg) BRT respectively. The expression of extracellular matrix in NRK-49F cells was induced by treatment with human transforming growth factor-β1 (recombined human TGF-β1)

and BRT-containing serum. Western blotting and Northern blotting were used to measure type I and III procollagen

MMP-9

and TIMP-1. The high dose BRT-containing serum could decrease the type I and III procollagen gene expression which boosted by TGF-β1

as indicated by its inhibition of type I and III procollagen and TIMP-1 expression.

关键词

renal interstitial fibroblastsBiejia Ruangan Tablettype I and III procollagenmatrix metalloproteinase -9tissue inhibitor of metalloproteinase -1

Keywords

renal interstitial fibroblastsBiejia Ruangan Tablettype I and III procollagenmatrix metalloproteinase -9tissue inhibitor of metalloproteinase -1

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