Inhibition of platelet aggregation and in vitro free radical scavenging activity of dried fruiting bodies of Pleurotus eous

S. R. Suseem; Mary Saral

doi:10.1007/s11655-014-1786-y

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Inhibition of platelet aggregation and in vitro free radical scavenging activity of dried fruiting bodies of Pleurotus eous

Updated：2021-08-27

- Inhibition of platelet aggregation and in vitro free radical scavenging activity of dried fruiting bodies of Pleurotus eous
- Chinese Journal of Integrative Medicine Vol. 21, Issue 7, Pages: 530-536(2015)
- Affiliations：
  
  Pharmaceutical Chemistry Division, School of Advanced Sciences, VIT University, Vellore-14,India
- Author bio：
- Funds：
- DOI：10.1007/s11655-014-1786-y
  CLC：
- Published：2015，
  
  Published Online：31 July 2014，
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Suseem, S.R., Saral, M. Inhibition of platelet aggregation and in vitro free radical scavenging activity of dried fruiting bodies of Pleurotus eous, ., Chin. J. Integr. Med. 21, 530–536 (2015). https://doi.org/10.1007/s11655-014-1786-y

S. R. Suseem, Mary Saral. Inhibition of platelet aggregation and in vitro free radical scavenging activity of dried fruiting bodies of Pleurotus eous. [J]. Chinese Journal of Integrative Medicine 21(7):530-536(2015)
Suseem, S.R., Saral, M. Inhibition of platelet aggregation and in vitro free radical scavenging activity of dried fruiting bodies of Pleurotus eous, ., Chin. J. Integr. Med. 21, 530–536 (2015). https://doi.org/10.1007/s11655-014-1786-y DOI：

S. R. Suseem, Mary Saral. Inhibition of platelet aggregation and in vitro free radical scavenging activity of dried fruiting bodies of Pleurotus eous. [J]. Chinese Journal of Integrative Medicine 21(7):530-536(2015) DOI： 10.1007/s11655-014-1786-y.

摘要

To evaluate the ethyl acetate

methanol and aqueous extracts of dried fruiting bodies of Pleurotus eous for its anti-platelet activity on human volunteer’s blood. And also to analyze the free radical scavenging property of the extracts of P.eous by using various in vitro models. Anti-platelet activity of dried fruiting bodies of P.eous was evaluated by in vitro model using blood platelets. Inhibition of platelet aggregation was monitored after pre-incubation of platelets with the crude extracts of mushroom P.eous. Antioxidant activities of extracts of P.eous were evaluated by different in vitro experiments

namely

1-diphenyl-2-picryl hydrazyl (DPPH)

superoxide

hydroxyl radical and lipid peroxide radical models. Crude extracts of mushroom P.eous inhibited platelet aggregation dose-dependently which was induced by adenosine diphosphate (ADP). At a maximum concentration of 10 mg/mL

methanol extract effected 64.02% inhibition of lipid per-oxidation and 50.12% scavenging effect on superoxide anion radical. Aqueous extract of P.eous have shown 69.43% chelating ability on ferrous ions

24.27% scavenging effect on hydroxyl radical and 49.57% scavenging effect on DPPH radical at 10 mg/mL. Increasing concentrations of the extract were found to cause progressively decreasing of the intensity of absorbance. Anti-platelet effects could be related in part to the polyphenolic compounds present in the extracts. Antioxidant activity results indicated the free radical scavenging property of the extracts of P.eous which might be due to the high content of phenolic compounds and flavonoids.

Abstract

To evaluate the ethyl acetate

namely

1-diphenyl-2-picryl hydrazyl (DPPH)

superoxide

关键词

anti-plateletPleurotus eousmushroomantioxidantscavenging effectchelating effectreductive

Keywords

anti-plateletPleurotus eousmushroomantioxidantscavenging effectchelating effectreductive

references

Dounia G, Mohamed B, Mohammed A, Abderrahim Z, Abdelkhaleq L, Chantal L, et al. Parsley extract inhibits in vitro and ex vivo platelet aggregation and prolongs bleeding time in rats. J Ethnopharmacol 2009;125:170᾿74.

Hernandez HR, Armas de Hernandez MJ, Armas Padilla MC, Carrajal AR, Guerrero Pajuelo J. Platelet actions of calcium channel blockers in hypertension. Clin Cardiol 1997;20:1125᾿130.

Suseem SR, Mary Saral A, Neelakanda Reddy P, Marslin G. Evaluation of the analgesic activity of ethyl acetate, methanol and aqueous extracts of Pleurotus eous mushroom. Asian Pacific J Tropic Med 2011;4:117᾿20.

Suseem SR, Mary Saral A. Studies on anti-inflammatory and antipyretic activities of fruiting bodies of Pleurotus eous in experimental animals. Pharmacol Online 2011;1:721᾿27.

Haibin T, Fengguo X, Kai F, Guangren S, Xiaoxv G, Liwei S, et al. Structural characterization and in vitro antitumor activity of a novel polysaccharide isolated from the fruiting bodies of Pleurotus ostreatus. Bioresource Technol 2009;100:1682᾿686.

Jayakumar T, Thomas PA, Geraldine P. In-vitro antioxidant activities of an ethanolic extract of the oyster mushroom, Pleurotus ostreatus. Inno Food Sci Emerg Technol 2009;10:228᾿34.

Smiderle FR, Olsen LM, Carbonero ER, Marcon R, Baggio CH, Freitas CS, et al. A-3-O-methylated mannogalactans from Pleurotus pulmonarius: Structure and antinociceptive effect. Phytochemistry 2008;69:2731᾿736.

Nevcihan G, Cengiz S, Mustafa C, Halil SM. Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species. Food Chem Toxicol 2009;47:2381᾿388.

Lakshmi B, Ajith TA, Nayana J, Janardhanan KK. Antimutagenic activity of methanolic extract of Ganoderma lucidum and its effect on hepatic damage caused by benzopyrene. J Ethnopharmacol 2006;107:297᾿03.

Chopra RN, Nayar SL, Chopra IC, eds. Glossary of Indian medicinal plants. New Delhi: Catholic Press,1986:262:109.

Cotelle A, Bernier JL, Catteau JP, Pommery J, Wallet JC, Gaydou EM. Antioxidant properties of hydroxy-flavones. Free Radic Biol Med 1996;20:32᾿5.

Liu F, Ooi VEC, Chang ST. Free radical scavenging activity of mushroom polysaccharide extracts. Life Sci 1997;60:763᾿71.

Rajeshwar Y, Senthil Kumar GP, Gupta M, Upal KM. Studies on in vitro antioxidant activities of methanol extract of Mucuna pruriens (fabaceae) seeds. Europ Bull Drug Res 2005;13:31᾿9.

Arouma OI, Grootveld M, Halliwell B. The role of iron in ascorbate-dependent deoxyribose degradation. J Inorg Biochem 1987;29:289᾿99.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351᾿53.

Nayana J, Ajith A, Kainoor KJ. Antioxidant, antiinflammatory and antitumor activities of Pleurotus sajorcaju. Int J Med Mushrooms 2001;3:232.

Dinis TCP, Madeira VMC, Almeida LM. Action of phenolic derivatives as inhibitors of membrane lipid-peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161᾿69.

Nevcihan G, Cengiz S, Mustafa C, Halil Solak M. Antioxidant activities, metal contents, total phenolics and flavonoids of seven Morchella species. Food Chem Toxicol 2009;47:2381᾿388.

Olas B, Wachowicz B, Stochmal A, Oleszek W. Inhibition of blood platelet adhesion and secretion by different phenolics from Yucca Schidigera Roezl bark. Nutrition 2005;21:199᾿06.

Lata H, Ahuja G K. Role of free radicals in health and disease. Ind J Physio Allied Sci 2003;57:124.

Rajbir S, Sukhpreet S, Subodh K, Saroj A. Studies on antioxidant potential of methanol extract/fractions of Acacia auriculiformis A. Cunn. Food Chem 2007;103:505᾿11.

Sanchez Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Tech Int 2002;8:122.

Khanam S, Shivprasad HN, Kshama D. In vitro antioxidant screening models: a review. Ind J Pharm Educ 2004;38:180.

Shirwaikar A, Rajendran K, Dinesh Kumar C. In vitro antioxidant studies of Annona squamosa Linn Leaves. Ind J Exp Biol 2004;42:803.

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