Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer

Dong Gyu Lee; Sung-Il Jang; Young-Rang Kim; Kyeong Eun Yang; So Jung Yoon; Zee-Won Lee; Hyun Joo An; Ik-Soon Jang; Jong-Soon Choi; Hwa-Seung Yoo

doi:10.1007/s11655-014-1789-8

Your Location：

Home >

Browse articles >

Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer

Updated：2021-08-27

- Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer
- Chinese Journal of Integrative Medicine Vol. 22, Issue 5, Pages: 344-352(2016)
- Affiliations：
  
  1. Division of Life Science, Korea Basic Science Institute,Daejeon,Korea
  2. East-West Cancer Center, Dunsan Oriental Hospital of Daejeon University,Daejeon,Korea
  3. Graduate School of Analytical Science and Technology, Chungnam National University,Daejeon,Korea
- Author bio：
- Funds：
  
  Equally contributed to this work
- DOI：10.1007/s11655-014-1789-8
  CLC：
- Published：2016，
  
  Published Online：26 August 2014，
Scan for full text
Lee, D.G., Jang, SI., Kim, YR. et al. Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer., Chin. J. Integr. Med. 22, 344–352 (2016). https://doi.org/10.1007/s11655-014-1789-8

Dong Gyu Lee, Sung-Il Jang, Young-Rang Kim, et al. Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer. [J]. Chinese Journal of Integrative Medicine 22(5):344-352(2016)
Lee, D.G., Jang, SI., Kim, YR. et al. Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer., Chin. J. Integr. Med. 22, 344–352 (2016). https://doi.org/10.1007/s11655-014-1789-8 DOI：

Dong Gyu Lee, Sung-Il Jang, Young-Rang Kim, et al. Anti-proliferative effects of ginsenosides extracted from mountain ginseng on lung cancer. [J]. Chinese Journal of Integrative Medicine 22(5):344-352(2016) DOI： 10.1007/s11655-014-1789-8.

摘要

To investigate the effect of three major ginsenosides from mountain ginseng as anticancer substance and explore the underlying mechanism involved in lung cancer. The inhibitory proliferation of lung cancer by major five ginsenosides (Rb1

Rb2

Rg1

and Re) was examined using 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyl tetrazolium bromide assay. Calculated 50% inhibition (IC50) values of five ginsenosides were determined and compared each other. Apoptosis by the treatment of single ginsenoside was performed by fluorescence-assisted cytometric spectroscopy. The alterations of apoptosis-related proteins were evaluated by Western blot analysis. The abundance of ginsenosides in butanol extract of mountain ginseng (BX-MG) was revealed in the order of Rb1

Rg1

Rc and Rb2. Among them

Rb1 was the most effective to lung cancer cell

followed by Rb2 and Rg1 on the basis of relative IC50 values of IMR90 versus A549 cell. The alterations of apoptotic proteins were confirmed in lung cancer A549 cells according to the administration of Rb1

Rb2 and Rg1. The expression levels of caspase-3 and caspase-8 were increased upon the treatment of three ginsenosides

however

the levels of caspase-9 and anti-apoptotic protein Bax were not changed. Major ginsenosides such as Rb1

Rb2 and Rg1 comprising BX-MG induced apoptosis in lung cancer cells via extrinsic apoptotic pathway rather than intrinsic mitochondrial pathway.

Abstract

Rb2

Rg1

and Re) was examined using 3-(4

5-dimethylthiazol-2-yl)-2

Rg1

Rc and Rb2. Among them

Rb1 was the most effective to lung cancer cell

Rb2 and Rg1. The expression levels of caspase-3 and caspase-8 were increased upon the treatment of three ginsenosides

however

the levels of caspase-9 and anti-apoptotic protein Bax were not changed. Major ginsenosides such as Rb1

Rb2 and Rg1 comprising BX-MG induced apoptosis in lung cancer cells via extrinsic apoptotic pathway rather than intrinsic mitochondrial pathway.

关键词

Butanol extract of mountain ginsengGinsenosidelung cancerapoptosis

Keywords

Butanol extract of mountain ginsengGinsenosidelung cancerapoptosis

references

Jia L, Zao YQ. Current evaluation of the millennium phytomedicine-ginseng (1): etymology, pharmacognosy, phytochemistry, market and regulations. Curr Med Chem 2009;16:2475–2484.

Yun TK, Lee YS, Lee YH, Kim SI, Yun HY. Anticarcinogenic effect of Panax ginseng C. A. Meyer and identification of active compounds. J Kor Med Sci 2001;16(Suppl):S6–S18.

Hu LY, Fan YP. Review of study on the mechanism of active ingredients of single Chinese drug in treating intracerebral hemorrhage. Chin J Integr Tradit West Med (Chin) 2006;26:376–380.

Han EJ, Kim YS, Yu KW, Jeong CS, Paek KY. Adventitious root cultures of Panax ginseng C. B. Meyer and ginsenoside production through large-scale bioreactor system. J Plant Biotech 2003;5:1–6.

Yoo BS, Chang MS, Byun SY. Characterization of cell cultures and ginsenoside production by cultured ginseng and wild mountain ginseng. Kor J Biotech Bioeng 2003;18:133–139.

Chang Y, Huang WJ, Tieen LT, Wang SJ. Ginsenosides Rg1 and Rb1 enhance glutamate release through activation of protein kinnase A in rat cerebrocortical nerve terminals (synaptosomes). Eur J Pharmacol 2008;578:28–36.

Chuang WC, Wu HK, Sheu SJ, Chiou SH, Chang HC, Chen YP. A comparative study on commercial samples of Rinseng radix. Planta Med 1995;61:459–465.

Wang CZ, Yuan CS. Potential role of ginseng in the treatment of colorectal cancer. Am J Chin Med 2008;36:1019–1028.

Li BH, Wang CZ, He TC, Yuan CS, Du W. Antioxidants potentiate American ginseng-induced killing of colorectal cancer cells. Cancer Lett 2010;289:62–70.

Nakata H, Kikuchi Y, Tode T, Hirata J, Kita T, Ishii K, et al. Inhibitory effects of ginsenoside Rh2 on tumor growth in nude mice bearing human ovarian cancer cells. Cancer Sci 1998;89:733–740.

Oh M, Choi YH, Choi S, Chung H, Kim K, Kim SI,et al. Anti-proliferating effects of ginsenoside Rh2 on MCF-7 human breast cancer cells. Int J Oncol 1999;14:869–875.

Wang CZ, Zhang B, Song WX, Wang A, Ni M, Luo X, et al. Steamed American ginseng berry: ginsenoside analyses and anticancer activities. J Agr Food Chem 2006;54:9936–9942.

Zhang Q, Kang X, Zhao W. Antiangiogenic effect of lowdose cyclophosphamide combined with ginsenoside Rg3 on Lewis lung carcinoma. Biochim Biophys Res Comm 2006;342:824–828.

Liu TG, Huang Y, Cui DD, Huang XB, Mao SH, Ji LL,et al. Inhibitory effect of ginsenoside Rg3 combined with gemcitabine on angiogenesis and growth of lung cancer in mice. BMC Cancer 2009;23:250.

Lu P, Su W, Miao ZH, Niu HR, Liu J, Hua QL. Effect and mechanism of ginsenoside Rg3 on postoperative life span of patients with non-small cell lung cancer. Chin J Integr Med 2008;14:33–36.

Hwang JW, Oh JH, Yoo HS, Lee YW, Cho CK, Kwo KR,et al. Mountain ginseng extract exhibits anti-lung cancer activity by inhibiting the nuclear translocation of NF-κB. Am J Chin Med 2012;40:187–202.

Al HH, Wheatley-Price P, Clemons M, Shepherd FA. Prevention and management of bone metastases in lung cancer: a review. J Thorac Oncol 2009;4:251–259.

Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ. Cancer statistics. CA Cancer J Clin 2009;59:225–249.

Lee JH, Kwon KR, Cho CK, Han SS, Yoo HS. Advanced cancer cases treated with cultivated wild ginseng pharmacopuncture. J Acupunct Meridi Stud 2010;3:119–124.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth 1983;65:55–63.

Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pahrmacol 1999;58:1685–1693.

Gillis CN. Panax ginseng pharmacology: a nitric oxide link? Biochem Pharmacol 1997;54:1–8.

Shin HR, Kim JY, Yun TK, Morgan G, Vainio H. The cancer-preventive potential of Panax ginseng: a review of human and experimental evidence. Cancer Causes Control 2000;11:565–576.

Koo HN, Jeong HJ, Choi IY, An HJ, Moon PD, Kim SJ,et al. Mountain grown ginseng induces apoptosis in HL-60 cells and its mechanism have little relation with TNF-alpha production. Am J Chin Med 2007;35:169–182.

Qi LW, Wang CZ, Yuan CS. American ginseng: potential structure-function relationship in cancer chemoprevention. Biochem Pharmacol 2010;80:947–954.

Sato K, Mochizuki M, Saiki I, Yoo YC, Samukawa K, Azuma I. Inhibition of tumor angiogenesis and metastasis by a saponin of Panax ginseng, ginsenoside-Rb2. Biol Pharm Bull 1994;17:635–639.

Shinkai K, Akedo H, Mukai M, Imamura F, Isoai A, Kobayashi M, et al. Inhibition of in vitro tumor cell invasion by ginsenoside Rg3. Jpn K Cancer Res 1996;87:357–362.

Cheng CC, Yang SM, Huang CY, Chen JC, Chang WM, Hsu SL. Molecular mechanisms of ginsenoside Rh2-mediated G1 growth arrest and apoptosis in human lung adenocarcinoma A549 cells. Cancer Chemother Pharmacol 2005;55:531–540.

Chen MW, Ni L, Zhao XG, Niu XY. The inhibition of 20(R)-ginsenoside Rg3 on the expression of angiogenesis factors proteins in human lung carcinoma cell line A549 and HUVEC304 cell. Chin J Mater Med (Chin) 2005;30:357–360.

Wang W, Rayburn ER, Hang J, Zhao Y, Wang H, Zhang R. Anti-lung cancer effects of novel ginsenoside 25-OCH(3)-PPD. Lung Cancer 2009;65:306–311.

Mohamad N, Gutiérrez A, Núñez M, Cocca C, Martín G, Cricco G, et al. Mitochondrial apoptotic pathways. Biocell 2005;29:149–161.

Cho SH, Chung KS, Choi JH, Kim DH, Lee KT. Compound K, a metabolite of ginseng saponin, induces apoptosis via caspase-8-dependent pathway in HL-60 human leukemia cells. BMC Cancer 2009;9:449.

FULL TEXT LINK

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells

The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells

Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells

Arsenic disulfide induced apoptosis and concurrently promoted erythroid differentiation in cytokine-dependent myelodysplastic syndrome-progressed leukemia cell line F-36p with complex karyotype including monosomy 7

Related Author

No data

Related Institution

Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran

Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran

Department of Anesthesia, Chi-Mei Medical Center, Liouying, Tainan

Department of Nursing, Min-Hwei College of Health Care Management, Tainan

Division of Endocrinology and Metabolism, Kaohsiung Veteran General Hospital Tainan Branch, Tainan

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰