Effect of panax notoginseng saponins injection on the p38MAPK pathway in lung tissue in a rat model of hypoxic pulmonary hypertension

Shan Zhao; Meng-xiao Zheng; Hai-e Chen; Cheng-yun Wu; Wan-tie Wang

doi:10.1007/s11655-014-1790-2

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Effect of panax notoginseng saponins injection on the p38MAPK pathway in lung tissue in a rat model of hypoxic pulmonary hypertension

Updated：2021-08-27

- Effect of panax notoginseng saponins injection on the p38MAPK pathway in lung tissue in a rat model of hypoxic pulmonary hypertension
- Chinese Journal of Integrative Medicine Vol. 21, Issue 2, Pages: 147-151(2015)
- Affiliations：
  
  1. Department of Pathophysiology, Basic Medicine College, Wenzhou Medical University,Wenzhou,China
  2. Department of Respiratory Disease, The Second Affiliated Hospital, Wenzhou Medical University,Wenzhou,China
- Author bio：
- Funds：
  
  Supported by the Key Technology Development Foundation of Zhejiang Province, China (No. 2006C33073) and the Key Projects of Traditional Chinese Medicine Development Plan of Zhejiang Province, China (No. 2008ZA017, 2013ZZ011)
- DOI：10.1007/s11655-014-1790-2
  CLC：
- Published：2015，
  
  Published Online：19 December 2014，
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Zhao, S., Zheng, Mx., Chen, He. et al. Effect of panax notoginseng saponins injection on the p38MAPK pathway in lung tissue in a rat model of hypoxic pulmonary hypertension., Chin. J. Integr. Med. 21, 147–151 (2015). https://doi.org/10.1007/s11655-014-1790-2

Shan Zhao, Meng-xiao Zheng, Hai-e Chen, et al. Effect of panax notoginseng saponins injection on the p38MAPK pathway in lung tissue in a rat model of hypoxic pulmonary hypertension. [J]. Chinese Journal of Integrative Medicine 21(2):147-151(2015)
Zhao, S., Zheng, Mx., Chen, He. et al. Effect of panax notoginseng saponins injection on the p38MAPK pathway in lung tissue in a rat model of hypoxic pulmonary hypertension., Chin. J. Integr. Med. 21, 147–151 (2015). https://doi.org/10.1007/s11655-014-1790-2 DOI：

Shan Zhao, Meng-xiao Zheng, Hai-e Chen, et al. Effect of panax notoginseng saponins injection on the p38MAPK pathway in lung tissue in a rat model of hypoxic pulmonary hypertension. [J]. Chinese Journal of Integrative Medicine 21(2):147-151(2015) DOI： 10.1007/s11655-014-1790-2.

摘要

To investigate the effect of panax notoginseng saponins (PNS) injection on pulmonary artery pressure and the expression of p38MAPK in lung tissue of rats subjected to chronic hypoxia. Thirty adult male Sprague Dawley rats were randomly divided into three groups (ten in each group): rats in control group were exposed to normoxic condition and the rats in hypoxia group and PNS group were subjected to 4-week hypoxia

and PNS injection (50 mg·kg−1·d−1) was administrated intraperitoneally at 30 min in the PNS group daily before the rats were kept in the hypoxic chamber

while rats in the other two groups received equal dose of normal saline instead. After chronic hypoxia

mean pulmonary artery pressure (mPAP) and mean carotid artery pressure (mCAP) were measured. The heart and lung tissues were harvested

and right ventricle (RV) and left ventricle plus ventricular septum (LV+S) were weighed to calculate the ratio of RV/(LV+S). The expression of p38MAPK mRNA was determined by reverse transcription-polymerase chain reaction

the quantity of phosphorylated p38MAPK (p-p38MAPK) in rat lung tissues and pulmonary arterioles was determined by Western blot and immunohistochemistry. Compared with the control group

mPAP and the ratio of RV/(LV+S) in the hypoxia group were increased

the expression of p-p38MAPK in pulmonary arterioles and p38MAPK mRNA in the lung were higher (P<0.05). The changes of these parameters in the hypoxia group were significantly attenuated by PNS treatment (P<0.05). PNS injection was shown to prevent hypoxic pulmonary hypertension at least partly by regulating p38MAPK pathway.

Abstract

and PNS injection (50 mg·kg−1·d−1) was administrated intraperitoneally at 30 min in the PNS group daily before the rats were kept in the hypoxic chamber

while rats in the other two groups received equal dose of normal saline instead. After chronic hypoxia

mean pulmonary artery pressure (mPAP) and mean carotid artery pressure (mCAP) were measured. The heart and lung tissues were harvested

the quantity of phosphorylated p38MAPK (p-p38MAPK) in rat lung tissues and pulmonary arterioles was determined by Western blot and immunohistochemistry. Compared with the control group

mPAP and the ratio of RV/(LV+S) in the hypoxia group were increased

关键词

panax notoginseng saponinshypoxiapulmonary arterial hypertensionp38MAPK

Keywords

panax notoginseng saponinshypoxiapulmonary arterial hypertensionp38MAPK

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