Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway

Hu Yan; You-xin Su; Xue-yi Lin; Bao-jun Chen; Qing Zhang; Zi-yi Zhang; Yi-ru Wang; Ya-nan Li; Mei-li Lu; Zhen He; Lu Sheng; Wen-ting Wang

doi:10.1007/s11655-014-1801-y

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Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway

Updated：2021-08-27

- Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway
- Chinese Journal of Integrative Medicine Vol. 20, Issue 5, Pages: 353-359(2014)
- Affiliations：
  
  Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072828)
- DOI：10.1007/s11655-014-1801-y
  CLC：
- Published：2014，
  
  Published Online：30 January 2014，
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Yan, H., Su, Yx., Lin, Xy. et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway., Chin. J. Integr. Med. 20, 353–359 (2014). https://doi.org/10.1007/s11655-014-1801-y

Hu Yan, You-xin Su, Xue-yi Lin, et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway. [J]. Chinese Journal of Integrative Medicine 20(5):353-359(2014)
Yan, H., Su, Yx., Lin, Xy. et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway., Chin. J. Integr. Med. 20, 353–359 (2014). https://doi.org/10.1007/s11655-014-1801-y DOI：

Hu Yan, You-xin Su, Xue-yi Lin, et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway. [J]. Chinese Journal of Integrative Medicine 20(5):353-359(2014) DOI： 10.1007/s11655-014-1801-y.

摘要

To evaluate the effect of Zhuanggu Jianxi Decoction (壮骨健膝方

ZGJXD) on interleukin-1 β (IL-1 β)-induced degeneration of chondrocytes (CDs) as well as the activation of caveolin-p38 mitogen-activated protein kinase (MAPK) signal pathway

investigating the possible molecular mechanism that ZGJXD treats osteoarthritis. Serum pharmacology was applied in the present study

where ZGJXD was orally administrated to New Zealand rabbits and then ZGJXD containing serum (ZGJXD-S) was collected for following in vitro experiments. CDs were isolated aseptically from New Zealand rabbits and then cultured in vitro. Upon IL-1 β stimulation

the degeneration of CDs was verified by inverted microscope

toluidine blue stain and type II collagen immunocytochemistry. After IL-1 β-stimulated CDs were intervened with blank control serum

ZGJXD-S

together with or without SB203580 (a specific inhibitor of p38 MAPK) for 48 h

caveolin-1 protein expression and the phosphorylation level of p38 were determined by Western blotting

and the mRNA expression of IL-1 β

tumor necrosis factor α (TNF-α)

matrix metalloproteinase 3 (MMP-3) and MMP-13 were examined by real-time polymerase chain reaction. IL-1 β stimulation induced degeneration of CDs

increased caveolin-1 expression and p38 phosphorylation

up-regulated the mRNA level of IL-1 β

TNF-α

MMP-3 and MMP-13. However

the IL-1 β-induced activation of caveolin-p38 signaling and alteration in the expression of p38 downstream target genes were suppressed by ZGJXD-S and/or SB203580 in CDs. ZGJXD can prevent CDs degeneration via inhibition of caveolin-p38 MAPK signal pathway

which might be one of the mechanisms that ZGJXD treats osteoarthritis.

Abstract

To evaluate the effect of Zhuanggu Jianxi Decoction (壮骨健膝方

ZGJXD) on interleukin-1 β (IL-1 β)-induced degeneration of chondrocytes (CDs) as well as the activation of caveolin-p38 mitogen-activated protein kinase (MAPK) signal pathway

investigating the possible molecular mechanism that ZGJXD treats osteoarthritis. Serum pharmacology was applied in the present study

the degeneration of CDs was verified by inverted microscope

toluidine blue stain and type II collagen immunocytochemistry. After IL-1 β-stimulated CDs were intervened with blank control serum

ZGJXD-S

together with or without SB203580 (a specific inhibitor of p38 MAPK) for 48 h

caveolin-1 protein expression and the phosphorylation level of p38 were determined by Western blotting

and the mRNA expression of IL-1 β

tumor necrosis factor α (TNF-α)

matrix metalloproteinase 3 (MMP-3) and MMP-13 were examined by real-time polymerase chain reaction. IL-1 β stimulation induced degeneration of CDs

increased caveolin-1 expression and p38 phosphorylation

up-regulated the mRNA level of IL-1 β

TNF-α

MMP-3 and MMP-13. However

which might be one of the mechanisms that ZGJXD treats osteoarthritis.

关键词

osteoarthritisZhuanggu Jianxi Decoctionchondrocyte degenerationcaveolin-p38 pathwayserum pharmacology

Keywords

osteoarthritisZhuanggu Jianxi Decoctionchondrocyte degenerationcaveolin-p38 pathwayserum pharmacology

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Wangjing Hospital, China Academy of Chinese Medical Sciences

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Tianjin University of Traditional Chinese Medicine

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