Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells

Qian Chen; Qin Zhuang; Wei Mao; Xiao-ming Xu; Li-hui Wang; Hai-bing Wang

doi:10.1007/s11655-014-1810-x

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Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells

Updated：2021-08-27

- Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells
- Chinese Journal of Integrative Medicine Vol. 20, Issue 10, Pages: 743-750(2014)
- Affiliations：
  
  1. Department of Cardiology, First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
  2. Lab, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou,Central,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81170270) and Medicine and Technology Program of Zhejiang Province (No. 2013KYB188)
- DOI：10.1007/s11655-014-1810-x
  CLC：
- Published：2014，
  
  Published Online：16 April 2014，
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Chen, Q., Zhuang, Q., Mao, W. et al. Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells., Chin. J. Integr. Med. 20, 743–750 (2014). https://doi.org/10.1007/s11655-014-1810-x

Qian Chen, Qin Zhuang, Wei Mao, et al. Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells. [J]. Chinese Journal of Integrative Medicine 20(10):743-750(2014)
Chen, Q., Zhuang, Q., Mao, W. et al. Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells., Chin. J. Integr. Med. 20, 743–750 (2014). https://doi.org/10.1007/s11655-014-1810-x DOI：

Qian Chen, Qin Zhuang, Wei Mao, et al. Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells. [J]. Chinese Journal of Integrative Medicine 20(10):743-750(2014) DOI： 10.1007/s11655-014-1810-x.

摘要

To investigate the anti-angiogenic effect of cryptotanshinone (CPT) on human umbilical vein endothelial cells (HUVECs) and the effect of CPT on Wnt/β-catenin signaling pathway. HUVECs were incubated with 0

2.5

and 20 μ mol/L CPT for detecting cell viability with dimethyl thiazolyl-2

5-diphenyltetrazolium bromide (MTT) assay. Then

HUVECs were incubated with 0

2.5

and 10 μ mol/L CPT for detecting endothelial cell migration

invasion

and tubular-like structure formation with wound healing

transwell invasion and matrigel tube formation assays

respectively. To gain insight into CPT-mediated signaling

the effects of CPT on T-cell factor/lymphocyte enhancer factor (TCF/LEF) transcription factors were detected by the Dual-luciferase reporter assay. Next

the nuclear expression of β-catenin was evaluated using Western blot and immunochemistry. Finally

vascular endothelial growth factor (VEGF) and cyclin D1

downstream proteins of the Wnt pathway were examined with Western blot. CPT dose-dependently suppressed endothelial cell viability

migration

invasion

and tubular-like structure formation. In particular

CPT blocked β-catenindependent transcription in HUVECs in a dose-dependent manner. In Western bolt

10 μ mol/L CPT decreased expression of β-catenin in nucleus of HUVECs (P<0.01). In immunohistochemistry

β-catenin was more potent in response to LiCl (an activator of the pathway) treatment. However

the signals were weaker in the nucleus of the CPT (10 μ mol/L) group

compared to the positive control. Also

VEGF and cyclin D1 were both eliminated by CPT in 5 and 10 μ mol/L doses (P<0.05). Our study supported the role of CPT as an angiogenic inhibitor

which may impact on the Wnt/β-catenin signaling pathway.

Abstract

2.5

and 20 μ mol/L CPT for detecting cell viability with dimethyl thiazolyl-2

5-diphenyltetrazolium bromide (MTT) assay. Then

HUVECs were incubated with 0

2.5

and 10 μ mol/L CPT for detecting endothelial cell migration

invasion

and tubular-like structure formation with wound healing

transwell invasion and matrigel tube formation assays

respectively. To gain insight into CPT-mediated signaling

the effects of CPT on T-cell factor/lymphocyte enhancer factor (TCF/LEF) transcription factors were detected by the Dual-luciferase reporter assay. Next

the nuclear expression of β-catenin was evaluated using Western blot and immunochemistry. Finally

vascular endothelial growth factor (VEGF) and cyclin D1

downstream proteins of the Wnt pathway were examined with Western blot. CPT dose-dependently suppressed endothelial cell viability

migration

invasion

and tubular-like structure formation. In particular

CPT blocked β-catenindependent transcription in HUVECs in a dose-dependent manner. In Western bolt

10 μ mol/L CPT decreased expression of β-catenin in nucleus of HUVECs (P<0.01). In immunohistochemistry

β-catenin was more potent in response to LiCl (an activator of the pathway) treatment. However

the signals were weaker in the nucleus of the CPT (10 μ mol/L) group

compared to the positive control. Also

VEGF and cyclin D1 were both eliminated by CPT in 5 and 10 μ mol/L doses (P<0.05). Our study supported the role of CPT as an angiogenic inhibitor

which may impact on the Wnt/β-catenin signaling pathway.

关键词

cryptotanshinoneangiogenesisWnt/β-catenin signaling pathwayHuman Umbilical Vein Endothelial Cellatherosclerosis

Keywords

cryptotanshinoneangiogenesisWnt/β-catenin signaling pathwayHuman Umbilical Vein Endothelial Cellatherosclerosis

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Center of Cardio-vascular Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao

School of Chinese Medicine, The Chinese University of Hong Kong

Laboratory of Cardiovascular Diseases, Xiyuan Hospital China Heart Institute of Chinese Medicine, China Academy of Chinese Medical Sciences

Clinical Medical College, Beijing University of Chinese Medicine

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