Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence

Ming Wang; Yan Lei

doi:10.1007/s11655-014-1814-6

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Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence

Updated：2021-08-27

- Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence
- Chinese Journal of Integrative Medicine Vol. 20, Issue 10, Pages: 758-763(2014)
- Affiliations：
  
  1. The Cardiology Department of Chongqing Hospital of Traditional Chinese Medicine and Chongqing the First Hospital,Chongqing,China
  2. The Experimental Research Center, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30973828), Major State Basic Research Development Program of China (937 Program, No. 2007CB507400), and Self-Project Foundation from China Academy of Chinese Medical Sciences (No. Z02151)
- DOI：10.1007/s11655-014-1814-6
  CLC：
- Published：2014，
  
  Published Online：30 July 2014，
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Wang, M., Lei, Y. Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence., Chin. J. Integr. Med. 20, 758–763 (2014). https://doi.org/10.1007/s11655-014-1814-6

Ming Wang, Yan Lei. Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence. [J]. Chinese Journal of Integrative Medicine 20(10):758-763(2014)
Wang, M., Lei, Y. Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence., Chin. J. Integr. Med. 20, 758–763 (2014). https://doi.org/10.1007/s11655-014-1814-6 DOI：

Ming Wang, Yan Lei. Time-effect relationship of extracts from ginseng, notoginseng and chuanxiong on vascular endothelial cells senescence. [J]. Chinese Journal of Integrative Medicine 20(10):758-763(2014) DOI： 10.1007/s11655-014-1814-6.

摘要

To observe the time-effect relation of extracts from ginseng

notoginseng and chuanxiong on angiotensin II (Ang II)-induced senescence of vascular endothelial cells and explore the feature of Chinese medicine against vascular diseases. Human umbilical vein endothelial cells (HUVECs) cultured in vitro were stimulated with 10−6 mol/L AngII to induce cell senescence

which were divided into 4 groups

the blank control group

the Ang II model group

the extracts group and the telmisartan group. The β-gal was used to identify senescence of cells

the cell counting kit-8 method was applied to assess the cell viability

the cell function was examined with the level of endothelial nitric oxide synthase (eNOS) and the flow cytometry was used for analyzing the cell cycle changes. Compared with the control cells

the cells positive for β-gal staining was significantly increased in the Ang II model group

and showed cell cycle arrest at G0/G1 phase with decreased S and G2/M phase cell percentage

eNOS expression and cell viability (P<0.05). The extracts and telmisartan treatment of Ang II-induced cells resulted in decreased β-gal positive cells with a reduction in G0/G1 phase cells and an increasing in S

G2/M phase cells and eNOS expression (P<0.05). At 24 h

the extracts were more effective than telmisartan (P<0.05); while telmisartan was more effective at 48 h (P<0.05). Extracts from ginseng

notoginseng and chuanxiong can delay Ang II-induced aging of HUVECs and may play an important role in early senescence.

Abstract

To observe the time-effect relation of extracts from ginseng

which were divided into 4 groups

the blank control group

the Ang II model group

the extracts group and the telmisartan group. The β-gal was used to identify senescence of cells

the cell counting kit-8 method was applied to assess the cell viability

the cell function was examined with the level of endothelial nitric oxide synthase (eNOS) and the flow cytometry was used for analyzing the cell cycle changes. Compared with the control cells

the cells positive for β-gal staining was significantly increased in the Ang II model group

and showed cell cycle arrest at G0/G1 phase with decreased S and G2/M phase cell percentage

G2/M phase cells and eNOS expression (P<0.05). At 24 h

the extracts were more effective than telmisartan (P<0.05); while telmisartan was more effective at 48 h (P<0.05). Extracts from ginseng

notoginseng and chuanxiong can delay Ang II-induced aging of HUVECs and may play an important role in early senescence.

关键词

ginsengnotoginsengChuanxiongangiotensin IIvascular endothelial cellssenescence

Keywords

ginsengnotoginsengChuanxiongangiotensin IIvascular endothelial cellssenescence

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