Effects of quercetin on hedgehog signaling in chronic myeloid leukemia KBM7 cells

Wei Li; Ying Zhao; Bo Tao; Ying Zhang

doi:10.1007/s11655-014-1817-3

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Effects of quercetin on hedgehog signaling in chronic myeloid leukemia KBM7 cells

Updated：2021-08-27

- Effects of quercetin on hedgehog signaling in chronic myeloid leukemia KBM7 cells
- Chinese Journal of Integrative Medicine Vol. 20, Issue 10, Pages: 776-781(2014)
- Affiliations：
  
  Department of Pharmacy, Harbin Commercial University,Harbin,China
- Author bio：
- Funds：
  
  Supported by grants of Heilongjiang Health Bureau Technology Project (No. 2011-520) and Harbin Technology Research Fund (No. 2012RFQYS073)
- DOI：10.1007/s11655-014-1817-3
  CLC：
- Published：2014，
  
  Published Online：13 June 2014，
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Li, W., Zhao, Y., Tao, B. et al. Effects of quercetin on hedgehog signaling in chronic myeloid leukemia KBM7 cells., Chin. J. Integr. Med. 20, 776–781 (2014). https://doi.org/10.1007/s11655-014-1817-3

Wei Li, Ying Zhao, Bo Tao, et al. Effects of quercetin on hedgehog signaling in chronic myeloid leukemia KBM7 cells. [J]. Chinese Journal of Integrative Medicine 20(10):776-781(2014)
Li, W., Zhao, Y., Tao, B. et al. Effects of quercetin on hedgehog signaling in chronic myeloid leukemia KBM7 cells., Chin. J. Integr. Med. 20, 776–781 (2014). https://doi.org/10.1007/s11655-014-1817-3 DOI：

Wei Li, Ying Zhao, Bo Tao, et al. Effects of quercetin on hedgehog signaling in chronic myeloid leukemia KBM7 cells. [J]. Chinese Journal of Integrative Medicine 20(10):776-781(2014) DOI： 10.1007/s11655-014-1817-3.

摘要

To investigate the effects of quercetin on Hedgehog (Hh) signaling in chronic myeloid leukemia KBM7 cells. The KBM7 cells were treated with 50

100 and 200 μmol/L quercetin for 48 h respectively. And then the trypan blue assay was used to examine the proliferative inhibition of quercetin. Apoptotic cells and cell cycle were measured by flow cytometry. The mRNA and protein expression were detected by quantitative real-time polymerase chain reaction (PCR) and Western blot

respectively. Quercetin significantly inhibited KBM7 cell proliferation

induced cell apoptosis

and blocked cell cycle at G1 phase

which were in dose-dependent manners. The mRNA and protein expression of Smoothened and Glioma1 (Gli1)

the members of Hh pathway decreased after treatment with quercetin. The Bcl-2 and Cyclin D1

targets of Hh signaling

also decreased after treatment with quercetin

respectively. Quercetin also could increase p53 and Caspase-3 expression. Bcr-abl mRNA copies decreased

but no changes of phosphorylated Bcr-abl and Bcr-abl proteins were observed

after treatment with quercetin. Quercetin could inhibit Hh signaling and its downstream targets in the KBM7 cells. And it might be one of mechanisms of inducing apoptosis and inhibiting cell cycle by quercetin.

Abstract

To investigate the effects of quercetin on Hedgehog (Hh) signaling in chronic myeloid leukemia KBM7 cells. The KBM7 cells were treated with 50

respectively. Quercetin significantly inhibited KBM7 cell proliferation

induced cell apoptosis

and blocked cell cycle at G1 phase

which were in dose-dependent manners. The mRNA and protein expression of Smoothened and Glioma1 (Gli1)

the members of Hh pathway decreased after treatment with quercetin. The Bcl-2 and Cyclin D1

targets of Hh signaling

also decreased after treatment with quercetin

respectively. Quercetin also could increase p53 and Caspase-3 expression. Bcr-abl mRNA copies decreased

but no changes of phosphorylated Bcr-abl and Bcr-abl proteins were observed

关键词

Quercetinhedgehog signalingChronic Myeloid Leukemiaapoptosiscell cycleBcr-abl

Keywords

Quercetinhedgehog signalingChronic Myeloid Leukemiaapoptosiscell cycleBcr-abl

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