Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model

Xian-hui Zhang; Shi-jing Huang; Yan-yun Wang; Ying Zhang; Ju-hua Pan; Jun Zheng; Duo-jiao Li; Xiao-ming Lei

doi:10.1007/s11655-014-1820-8

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Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model

Updated：2021-08-27

- Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model
- Chinese Journal of Integrative Medicine Vol. 21, Issue 3, Pages: 223-228(2015)
- Affiliations：
  
  Traditional Chinese Medicine Research and Development Center, Guang’anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072801 and 30672696)
- DOI：10.1007/s11655-014-1820-8
  CLC：
- Published：2015，
  
  Published Online：6 August 2014，
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Zhang, Xh., Huang, Sj., Wang, Yy. et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model., Chin. J. Integr. Med. 21, 223–228 (2015). https://doi.org/10.1007/s11655-014-1820-8

Xian-hui Zhang, Shi-jing Huang, Yan-yun Wang, et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model. [J]. Chinese Journal of Integrative Medicine 21(3):223-228(2015)
Zhang, Xh., Huang, Sj., Wang, Yy. et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model., Chin. J. Integr. Med. 21, 223–228 (2015). https://doi.org/10.1007/s11655-014-1820-8 DOI：

Xian-hui Zhang, Shi-jing Huang, Yan-yun Wang, et al. Effects of Kaixin Jieyu Decoction (开心解郁汤) on behavior and glial fibrillary acidic protein expression in cerebral hippocampus of a rat vascular depression model. [J]. Chinese Journal of Integrative Medicine 21(3):223-228(2015) DOI： 10.1007/s11655-014-1820-8.

摘要

To explore the effects and anti-depression mechanisms of Kaixin Jieyu Decoction (开心解郁汤

KJD). The rat vascular depression (VD) model was established by ligation of bilateral common carotid arteries (LBCCA) combined with chronic unpredictable mild stress (CUMS). Forty Wistar rats were randomly divided into sham

VD model

VD + high-dose KJD [15.4 g/(kg·d) of crude drug]

VD + medium-dose KJD [7.7 g/(kg·d) of crude drug]

and VD + fluoxetine [2.4 mg/(kg·d)] groups (n=8 in each group)

and the treatments lasted for 21 days. Changes of behavior and hippocampus pathology were observed. The level of glial fibrillary acidic protein (GFAP) protein and mRNA in hippocampus was detected respectively by immunohistochemistry and real-time polymerase chain reaction. Compared with the sham group

rats in model group showed a variety of behavioral obstacles

including a significant reduction in sucrose consumption percentage

horizontal and vertical activity scores in open-field tests (P<0.05 or P<0.01)

pathological damage like neuronal degeneration

necrosis

and a significant decrease of GFAP protein and mRNA in hippocampus (P<0.01); compared with the model group

rats in the high-dose KJD group

medium-dose KJD group and fluoxetine group obtained notable higher behavioral scores

and pathological injury lessened in hippocampus with a increased expression of GFAP protein and mRNA P<0.05 or P<0.01); compared with the medium-dose KJD group and fluoxetine group

GFAP mRNA in high-dose KJD group expressed higer (P<0.05). LBCCA combined with CUMS may cause depression-like behavioral changes resulting in the VD model of rats whose depression state can be ameliorated by KJD

and the mechanism of cerebral protection is related possibly with promoting expression of GFAP in hippocampus.

Abstract

To explore the effects and anti-depression mechanisms of Kaixin Jieyu Decoction (开心解郁汤

VD model

VD + high-dose KJD [15.4 g/(kg·d) of crude drug]

VD + medium-dose KJD [7.7 g/(kg·d) of crude drug]

and VD + fluoxetine [2.4 mg/(kg·d)] groups (n=8 in each group)

rats in model group showed a variety of behavioral obstacles

including a significant reduction in sucrose consumption percentage

horizontal and vertical activity scores in open-field tests (P<0.05 or P<0.01)

pathological damage like neuronal degeneration

necrosis

and a significant decrease of GFAP protein and mRNA in hippocampus (P<0.01); compared with the model group

rats in the high-dose KJD group

medium-dose KJD group and fluoxetine group obtained notable higher behavioral scores

and pathological injury lessened in hippocampus with a increased expression of GFAP protein and mRNA P<0.05 or P<0.01); compared with the medium-dose KJD group and fluoxetine group

and the mechanism of cerebral protection is related possibly with promoting expression of GFAP in hippocampus.

关键词

vascular depressionKaixin Jieyu DecoctionbehaviorGlial Fibrillary Acidic ProteinChinese Medicine

Keywords

vascular depressionKaixin Jieyu DecoctionbehaviorGlial Fibrillary Acidic ProteinChinese Medicine

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