Dan-gua Fang (丹瓜方) improves glycolipid metabolic disorders by promoting hepatic adenosine 5′-monophosphate activated protein kinase expression in diabetic Goto-Kakizaki rats

Yuan-long Lan; Su-ping Huang; Xian-pei Heng; Ling Chen; Peng-hui Li; Jing Wu; Liu-qing Yang; Xu-dong Pan; Tong Lin; Xin-ling Cheng; Qing Lin; Si-xin Chen

doi:10.1007/s11655-014-1826-2

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Dan-gua Fang (丹瓜方) improves glycolipid metabolic disorders by promoting hepatic adenosine 5′-monophosphate activated protein kinase expression in diabetic Goto-Kakizaki rats

Updated：2021-08-27

- Dan-gua Fang (丹瓜方) improves glycolipid metabolic disorders by promoting hepatic adenosine 5′-monophosphate activated protein kinase expression in diabetic Goto-Kakizaki rats
- Chinese Journal of Integrative Medicine Vol. 21, Issue 3, Pages: 188-195(2015)
- Affiliations：
  
  1. Fujian Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Zhangzhou Hospital of Traditional Chinese Medicine,Fujian Province,Zhangzhou,China
  3. Fujian University of Traditional Chinese Medicine,Fuzhou,China
  4. The People’s Hospital of Fujian Province, Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81173179), the Natural Science Foundation of Fujian Province (No. 2011J01198), the Fujian Medical Innovation Project (No. 2009-CX-19), the Research Foundation of Fujian Health Department (No. Zlcnfm02), the Fujian Provincial Department of Education Category A Projects (No. JA09131), the Fujian Health Department of Traditional Chinese Medicine Research (No. WZY0920), the CHEN Ke-ji Integrative Medicine Development Fund (No. CKJ2008047, CKJ2009004), and the Integrative Medicine of Fujian Key Laboratory of Age-related Diseases Funded Projects (No. 2008J1004-10)
- DOI：10.1007/s11655-014-1826-2
  CLC：
- Published：2015，
  
  Published Online：7 August 2014，
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Lan, Yl., Huang, Sp., Heng, Xp. et al. Dan-gua Fang (丹瓜方) improves glycolipid metabolic disorders by promoting hepatic adenosine 5′-monophosphate activated protein kinase expression in diabetic Goto-Kakizaki rats., Chin. J. Integr. Med. 21, 188–195 (2015). https://doi.org/10.1007/s11655-014-1826-2

Yuan-long Lan, Su-ping Huang, Xian-pei Heng, et al. Dan-gua Fang (丹瓜方) improves glycolipid metabolic disorders by promoting hepatic adenosine 5′-monophosphate activated protein kinase expression in diabetic Goto-Kakizaki rats. [J]. Chinese Journal of Integrative Medicine 21(3):188-195(2015)
Lan, Yl., Huang, Sp., Heng, Xp. et al. Dan-gua Fang (丹瓜方) improves glycolipid metabolic disorders by promoting hepatic adenosine 5′-monophosphate activated protein kinase expression in diabetic Goto-Kakizaki rats., Chin. J. Integr. Med. 21, 188–195 (2015). https://doi.org/10.1007/s11655-014-1826-2 DOI：

Yuan-long Lan, Su-ping Huang, Xian-pei Heng, et al. Dan-gua Fang (丹瓜方) improves glycolipid metabolic disorders by promoting hepatic adenosine 5′-monophosphate activated protein kinase expression in diabetic Goto-Kakizaki rats. [J]. Chinese Journal of Integrative Medicine 21(3):188-195(2015) DOI： 10.1007/s11655-014-1826-2.

摘要

To investigate the effect of Dan-gua Fang (丹瓜方) on adenosine 5′-monophosphate (AMP) activated protein kinase (AMPK) α expression in liver and subsequent improvement of glucose and lipid metabolism. Forty 13-week-old diabetic Goto-Kakizaki (GK) rats were randomly divided into model

Dan-gua Fang

metformin and simvastatin groups (n=10 for each)

and fed high-fat diet ad libitum. Ten Wistar rats were used as normal group and fed normal diet. After 24 weeks

liver expression of AMPKα mRNA was assessed by real-time PCR. AMPKα and phospho-AMPKα protein expression in liver was evaluated by Western blot. Liver histomorphology was carried out after hematoxylin-eosin staining

and blood glucose (BG)

glycosylated hemoglobin A1c (HbA1c)

food intake and body weight recorded. Similar AMPKα mRNA levels were found in the Dan-gua Fang group and normal group

slightly higher than the values obtained for the remaining groups (P<0.05). AMPKα protein expression in the Dan-gua Fang group animals was similar to other diabetic rats

whereas phospho-AMPKα (Thr-172) protein levels were markedly higher than in the metformin group and simvastatin group (P<0.05)

respectively. However

phosphor-AMPKα/AMPKα ratios were similar in all groups. Dan-gua Fang reduced fasting blood glucose with similar strength to metformin

and was superior in reducing cholesterol

triglycerides

high-density lipoprotein cholesterol as well as improving low-density lipoprotein cholesterol in comparison with simvastatin and metformin. Dan-gua Fang decreases plasma alanine aminotransferase (ALT) significantly. Dan-gua Fang

while treating phlegm-stasis

could decrease BG and lipid in type 2 diabetic GK rats fed with high-fat diet

and effectively protect liver histomorphology and function. This may be partly explained by increased AMPK expression in liver. Therefore

Dan-gua Fang might be an ideal drug for comprehensive intervention for glucose and lipid metabolism disorders in type 2 diabetes mellitus.

Abstract

Dan-gua Fang

metformin and simvastatin groups (n=10 for each)

and fed high-fat diet ad libitum. Ten Wistar rats were used as normal group and fed normal diet. After 24 weeks

and blood glucose (BG)

glycosylated hemoglobin A1c (HbA1c)

food intake and body weight recorded. Similar AMPKα mRNA levels were found in the Dan-gua Fang group and normal group

slightly higher than the values obtained for the remaining groups (P<0.05). AMPKα protein expression in the Dan-gua Fang group animals was similar to other diabetic rats

whereas phospho-AMPKα (Thr-172) protein levels were markedly higher than in the metformin group and simvastatin group (P<0.05)

respectively. However

phosphor-AMPKα/AMPKα ratios were similar in all groups. Dan-gua Fang reduced fasting blood glucose with similar strength to metformin

and was superior in reducing cholesterol

triglycerides

while treating phlegm-stasis

could decrease BG and lipid in type 2 diabetic GK rats fed with high-fat diet

and effectively protect liver histomorphology and function. This may be partly explained by increased AMPK expression in liver. Therefore

Dan-gua Fang might be an ideal drug for comprehensive intervention for glucose and lipid metabolism disorders in type 2 diabetes mellitus.

关键词

Dan-gua FangGoto-Kakizaki ratadenosine 5′-monophosphate activated protein kinasetype 2 diabetes mellitusliverglucose and lipid metabolism

Keywords

Dan-gua FangGoto-Kakizaki ratadenosine 5′-monophosphate activated protein kinasetype 2 diabetes mellitusliverglucose and lipid metabolism

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