Panax notoginseng saponins inhibits atherosclerotic plaque angiogenesis by down-regulating vascular endothelial growth factor and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 expression

Yun Qiao; Peng-ju Zhang; Xiao-ting Lu; Wei-wei Sun; Gui-lin Liu; Min Ren; Lei Yan; Ji-dong Zhang

doi:10.1007/s11655-014-1832-4

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Panax notoginseng saponins inhibits atherosclerotic plaque angiogenesis by down-regulating vascular endothelial growth factor and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 expression

Updated：2021-08-27

- Panax notoginseng saponins inhibits atherosclerotic plaque angiogenesis by down-regulating vascular endothelial growth factor and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 expression
- Chinese Journal of Integrative Medicine Vol. 21, Issue 4, Pages: 259-265(2015)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Qilu Hospital, Shandong University,Jinan,China
  2. Institution of Biochemistry and Molecular Biology, Medical School of Shandong University,Jinan,China
  3. Laboratory of Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University,Jinan,China
  4. Basic Medical College of Shandong University of Traditional Chinese Medicine,Jinan,China
  5. Second Hospital, Shandong University of Traditional Chinese Medicine,Jinan,China
- Author bio：
- Funds：
  
  Supported by the Plans for the Development of Traditional Chinese Medicine Science and Technology of Shandong Province (No. 2011-203)
- DOI：10.1007/s11655-014-1832-4
  CLC：
- Published：2015，
  
  Published Online：6 March 2014，
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Qiao, Y., Zhang, Pj., Lu, Xt. et al. Panax notoginseng saponins inhibits atherosclerotic plaque angiogenesis by down-regulating vascular endothelial growth factor and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 expression., Chin. J. Integr. Med. 21, 259–265 (2015). https://doi.org/10.1007/s11655-014-1832-4

Yun Qiao, Peng-ju Zhang, Xiao-ting Lu, et al. Panax notoginseng saponins inhibits atherosclerotic plaque angiogenesis by down-regulating vascular endothelial growth factor and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 expression. [J]. Chinese Journal of Integrative Medicine 21(4):259-265(2015)
Qiao, Y., Zhang, Pj., Lu, Xt. et al. Panax notoginseng saponins inhibits atherosclerotic plaque angiogenesis by down-regulating vascular endothelial growth factor and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 expression., Chin. J. Integr. Med. 21, 259–265 (2015). https://doi.org/10.1007/s11655-014-1832-4 DOI：

Yun Qiao, Peng-ju Zhang, Xiao-ting Lu, et al. Panax notoginseng saponins inhibits atherosclerotic plaque angiogenesis by down-regulating vascular endothelial growth factor and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 expression. [J]. Chinese Journal of Integrative Medicine 21(4):259-265(2015) DOI： 10.1007/s11655-014-1832-4.

摘要

To investigate the mechanism of Panax notoginseng saponins (PNS)

an effective component extracted from Panax notoginseng

on atherosclerotic plaque angiogenesis in atherosclerosis-prone apolipoprotein E-knockout (ApoE-KO) mice fed with high-fat

high-cholesterol diet. Twenty ApoE-KO mice were divided into two groups

the model group and the PNS group. Ten normal C57BL/6J mice were used as a control group. PNS (60 mg/kg) was orally administered daily for 12 weeks in the PNS group. The ratio of plaque area to vessel area was examined by histological staining. The tissue sample of aortic root was used to detect the CD34 and vascular endothelial growth factor (VEGF) expression areas by immunohistochemistry. The expression of VEGF and nicotinamide adenine dinucleotide phosphate oxidase subunit 4 (NOX4) were measured by reverse transcription polymerase chain reaction and Western blotting respectively. After treatment with PNS

the plaque areas were decreased (P<0.05). CD34 expressing areas and VEGF expression areas in plaques were significantly decreased (P<0.05). Meanwhile

VEGF and NOX4 mRNA expression were decreased after treatment with PNS. VEGF and NOX4 protein expression were also decreased by about 72% and 63%

respectively (P<0.01). PNS

which decreases VEGF and NOX4 expression

could alleviate plaque angiogenesis and attenuate atherosclerosis.

Abstract

To investigate the mechanism of Panax notoginseng saponins (PNS)

an effective component extracted from Panax notoginseng

on atherosclerotic plaque angiogenesis in atherosclerosis-prone apolipoprotein E-knockout (ApoE-KO) mice fed with high-fat

high-cholesterol diet. Twenty ApoE-KO mice were divided into two groups

the plaque areas were decreased (P<0.05). CD34 expressing areas and VEGF expression areas in plaques were significantly decreased (P<0.05). Meanwhile

VEGF and NOX4 mRNA expression were decreased after treatment with PNS. VEGF and NOX4 protein expression were also decreased by about 72% and 63%

respectively (P<0.01). PNS

which decreases VEGF and NOX4 expression

could alleviate plaque angiogenesis and attenuate atherosclerosis.

关键词

panax notoginseng saponinsatherosclerosisplaque angiogenesisvascular endothelial growth factornicotinamide adenine dinucleotide phosphate oxidase subunit 4

Keywords

panax notoginseng saponinsatherosclerosisplaque angiogenesisvascular endothelial growth factornicotinamide adenine dinucleotide phosphate oxidase subunit 4

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Department of Traditional Chinese Medicine, Shanghai No. 6 People’s Hospital, Affiliated to Shanghai Jiaotong University

Lab, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou

Department of Cardiology, First Affiliated Hospital of Zhejiang Chinese Medical University

Zhujiang Hospital, Southern Medical University

Integrative Hospital, College of Chinese Medicine, Southern Medical University

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