Inhibition mechanism of Qingluo Tongbi Granule (清络通痹颗粒) on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats

Tian-yang Liu; Ling-ling Zhou; Cong Zhou; Zhang-pu Liu; Chen Chen; Zhe Feng; Xue-ping Zhou

doi:10.1007/s11655-014-1839-x

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Inhibition mechanism of Qingluo Tongbi Granule (清络通痹颗粒) on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats

Updated：2021-08-27

- Inhibition mechanism of Qingluo Tongbi Granule (清络通痹颗粒) on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats
- Chinese Journal of Integrative Medicine Vol. 21, Issue 4, Pages: 291-298(2015)
- Affiliations：
  
  1. Nanjing University of Chinese Medicine,Nanjing,China
  2. Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072749), the Ministry of Education of Overseas Returnees Research Fund (No. 2006331), Six Talent Peaks Subject of Jiangsu Province (No. 2007), a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (No. 20116), Jiangsu Province Ordinary University Innovative Research Project and Ordinary University Innovative Research Project of Jiangsu Province (No. 2011)
- DOI：10.1007/s11655-014-1839-x
  CLC：
- Published：2015，
  
  Published Online：2 September 2014，
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Liu, Ty., Zhou, Ll., Zhou, C. et al. Inhibition mechanism of Qingluo Tongbi Granule (清络通痹颗粒) on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats., Chin. J. Integr. Med. 21, 291–298 (2015). https://doi.org/10.1007/s11655-014-1839-x

Tian-yang Liu, Ling-ling Zhou, Cong Zhou, et al. Inhibition mechanism of Qingluo Tongbi Granule (清络通痹颗粒) on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats. [J]. Chinese Journal of Integrative Medicine 21(4):291-298(2015)
Liu, Ty., Zhou, Ll., Zhou, C. et al. Inhibition mechanism of Qingluo Tongbi Granule (清络通痹颗粒) on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats., Chin. J. Integr. Med. 21, 291–298 (2015). https://doi.org/10.1007/s11655-014-1839-x DOI：

Tian-yang Liu, Ling-ling Zhou, Cong Zhou, et al. Inhibition mechanism of Qingluo Tongbi Granule (清络通痹颗粒) on osteoclast differentiation induced by synovial fibroblast and monocytes co-culture in adjuvant-induced arthritic rats. [J]. Chinese Journal of Integrative Medicine 21(4):291-298(2015) DOI： 10.1007/s11655-014-1839-x.

摘要

To study the mechanism underlying the inhibitory effect of Qingluo Tongbi Granule (清络通痹颗粒

QTG) on osteoclast differentiation in rheumatoid arthritis in rats. Fibroblast and monocyte co-culture were used to induce osteoclast differentiation in adjuvant-induced arthritic (AIA) rats. Serum containing QTG was prepared and added to the osteoclasts

and activation of the tumor necrosis factor receptor-associated factor 6/mitogen-activated protein kinase/nuclear factor of activated T cells

cytoplasmic1 (TRAF6/MAPK/NFATc1) pathways was examined. The induced osteoclasts were multinucleated and stained positive for tartrate-resistant acid phosphatase (TRAP) staining. Serum containing QTG at 14.4

7.2 or 3.6 g/kg inhibited the activation of TRAF6

extracellular regulated protein kinase (ERK)1/2

c-Jun N-terminal kinase (JNK) and p38 and decreased the percentage of cells with nuclear NFATc1 in a dose-dependent manner

the high and middle doses exhibited clear inhibitory activity (P<0.01 and P<0.05

respectively). After the addition of MAPK inhibitors

the NFATc1 expression showed no significant difference compared with the control group (P>0.05). Serum containing QTG could generally inhibit the TRAF6/MAPK pathways and possibly inhibit the NFATc1 pathway. In addition

QTG may regulate other signaling pathways that are related to osteoclast differentiation and maturation.

Abstract

To study the mechanism underlying the inhibitory effect of Qingluo Tongbi Granule (清络通痹颗粒

and activation of the tumor necrosis factor receptor-associated factor 6/mitogen-activated protein kinase/nuclear factor of activated T cells

7.2 or 3.6 g/kg inhibited the activation of TRAF6

extracellular regulated protein kinase (ERK)1/2

c-Jun N-terminal kinase (JNK) and p38 and decreased the percentage of cells with nuclear NFATc1 in a dose-dependent manner

the high and middle doses exhibited clear inhibitory activity (P<0.01 and P<0.05

respectively). After the addition of MAPK inhibitors

QTG may regulate other signaling pathways that are related to osteoclast differentiation and maturation.

关键词

Qingluo Tongbi Granulerheumatoid arthritisosteoclast differentiationsignaling pathwayregulation of tumor necrosis factor receptor-associated factor 6/mitogen-activated protein kinase pathwaysChinese Medicine

Keywords

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Department of Rheumatism Immunity, First Affiliated Hospital, Anhui University of Chinese Medicine

Anhui University of Chinese Medicine

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