Effects of Chinese Medicine Shen-Fu Injection (参附注射液) on the expression of inflammatory cytokines and complements during post-resuscitation immune dysfunction in a porcine model

Qian Zhang; Chun-sheng Li; Shuo Wang; Wei Gu

doi:10.1007/s11655-014-1857-8

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Effects of Chinese Medicine Shen-Fu Injection (参附注射液) on the expression of inflammatory cytokines and complements during post-resuscitation immune dysfunction in a porcine model

Updated：2021-08-27

- Effects of Chinese Medicine Shen-Fu Injection (参附注射液) on the expression of inflammatory cytokines and complements during post-resuscitation immune dysfunction in a porcine model
- Chinese Journal of Integrative Medicine Vol. 22, Issue 2, Pages: 101-109(2016)
- Affiliations：
  
  Department of Emergency Medicine, Beijing Chaoyang Hospital, Capital Medical University,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81372025)
- DOI：10.1007/s11655-014-1857-8
  CLC：
- Published：2016，
  
  Published Online：24 September 2014，
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Zhang, Q., Li, Cs., Wang, S. et al. Effects of Chinese Medicine Shen-Fu Injection (参附注射液) on the expression of inflammatory cytokines and complements during post-resuscitation immune dysfunction in a porcine model., Chin. J. Integr. Med. 22, 101–109 (2016). https://doi.org/10.1007/s11655-014-1857-8

Qian Zhang, Chun-sheng Li, Shuo Wang, et al. Effects of Chinese Medicine Shen-Fu Injection (参附注射液) on the expression of inflammatory cytokines and complements during post-resuscitation immune dysfunction in a porcine model. [J]. Chinese Journal of Integrative Medicine 22(2):101-109(2016)
Zhang, Q., Li, Cs., Wang, S. et al. Effects of Chinese Medicine Shen-Fu Injection (参附注射液) on the expression of inflammatory cytokines and complements during post-resuscitation immune dysfunction in a porcine model., Chin. J. Integr. Med. 22, 101–109 (2016). https://doi.org/10.1007/s11655-014-1857-8 DOI：

Qian Zhang, Chun-sheng Li, Shuo Wang, et al. Effects of Chinese Medicine Shen-Fu Injection (参附注射液) on the expression of inflammatory cytokines and complements during post-resuscitation immune dysfunction in a porcine model. [J]. Chinese Journal of Integrative Medicine 22(2):101-109(2016) DOI： 10.1007/s11655-014-1857-8.

摘要

To investigate the action of Shen-Fu Injection (参附注射液

SFI) in regulating the expression of the serum complements and inflammatory cytokines synthesized and released in response to the stress of global ischemia accompanying cardiac arrest (CA) and resuscitation. Thirty pigs were randomly divided into the sham (n=6) and 3 returns of spontaneous circulation (ROSC) groups (n=24). After 8-min untreated ventricular fibrillation and 2-min basic life support

24 pigs of the ROSC groups were randomized into three groups (n=8 per group)

which received central venous injection of SFI (SFI group)

epinephrine (EP group)

or saline (SA group). Hemodynamic status and blood samples were obtained at 0

0.5

and 24 h after ROSC. Serum concentrations of specific activation markers of the complement system C3

C4 and C5b-9 were increased during cardiopulmonary resuscitation throught 24 h after ROSC. There were intense changes of various pro-inflammatory cytokines and anti-inflammatory cytokines as early as 0.5 h after CA. Compared with the EP and SA groups

SFI treatment reduced the proinflammatory cytokines levels of interleukin (IL)-6

IL-8 and tumor necrosis factor α (TNF-α

P<0.05)

and increased the anti-inflammatory cytokine levels of IL-4 and IL-10 (P<0.05). Further

SFI treatment decreased the values of C3

C4 and C5b-9 compared with the EP and SA groups. SFI

derived from the ancient Chinese medicine

has significant effects in attenuating post-resuscitation immune dysfunction by modulating the expression of complements and cytokines levels. The current study provided an experimental basis for the clinical application of a potential pharmacologic target for post resuscitation immune dysfunction.

Abstract

To investigate the action of Shen-Fu Injection (参附注射液

24 pigs of the ROSC groups were randomized into three groups (n=8 per group)

which received central venous injection of SFI (SFI group)

epinephrine (EP group)

or saline (SA group). Hemodynamic status and blood samples were obtained at 0

0.5

and 24 h after ROSC. Serum concentrations of specific activation markers of the complement system C3

SFI treatment reduced the proinflammatory cytokines levels of interleukin (IL)-6

IL-8 and tumor necrosis factor α (TNF-α

P<0.05)

and increased the anti-inflammatory cytokine levels of IL-4 and IL-10 (P<0.05). Further

SFI treatment decreased the values of C3

C4 and C5b-9 compared with the EP and SA groups. SFI

derived from the ancient Chinese medicine

关键词

cardiac arrestcomplement activationcytokinesimmune dysfunction

Keywords

cardiac arrestcomplement activationcytokinesimmune dysfunction

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Related Author

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Related Institution

Emergency Department of Beijing Chaoyang Hospital, Capital Medical University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Department of Pharmacology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic

Institute of Pharmacology and Toxicology, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic

Department of Cardiology, Affiliated Hospital of Jining Medical College

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