Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway

Bin Yu; Cong-qi Dai; Zhen-you Jiang; En-qing Li; Chen Chen; Xian-lin Wu; Jia Chen; Qian Liu; Chang-lin Zhao; Jin-xiong He; Da-hong Ju; Xiao-yin Chen

doi:10.1007/s11655-014-1860-0

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Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway

Updated：2021-08-27

- Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway
- Chinese Journal of Integrative Medicine Vol. 20, Issue 7, Pages: 540-545(2014)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, School of Medicine, Jinan University,Guangzhou,China
  2. Shanghai Cancer Center and Shanghai Medical College, Fudan University,Shanghai,China
  3. Department of Microbiology and Immunology, School of Medicine, Jinan University,Guangzhou,China
  4. Shandong Provincial Corps Hospital of Chinese People’s Armed Police Forces,Jinan,China
  5. Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University,Guangzhou,China
  6. Department of Gynaecology and Obstetrics, Shenzhen Baoan Maternal and Child Health Hospital,Shenzhen,China
  7. Department of Oncology, Clifford Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
  8. Institute of Basic Theories of Traditional Chinese Medicine, China Academy of Traditional Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81273616 and 30973693), the Doctoral Fund of the Ministry of Education 2010 (No. 20104401110003) and the Natural Science Foundation of Guangdong Province (No. S2013010013434);The first two authors contributed equally to this work
- DOI：10.1007/s11655-014-1860-0
  CLC：
- Published：2014，
  
  Published Online：28 June 2014，
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Yu, B., Dai, Cq., Jiang, Zy. et al. Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway., Chin. J. Integr. Med. 20, 540–545 (2014). https://doi.org/10.1007/s11655-014-1860-0

Bin Yu, Cong-qi Dai, Zhen-you Jiang, et al. Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway. [J]. Chinese Journal of Integrative Medicine 20(7):540-545(2014)
Yu, B., Dai, Cq., Jiang, Zy. et al. Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway., Chin. J. Integr. Med. 20, 540–545 (2014). https://doi.org/10.1007/s11655-014-1860-0 DOI：

Bin Yu, Cong-qi Dai, Zhen-you Jiang, et al. Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway. [J]. Chinese Journal of Integrative Medicine 20(7):540-545(2014) DOI： 10.1007/s11655-014-1860-0.

摘要

To observe the anti-virus effects of andrographolide (AD) on the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) signaling pathway when immunological cells were infected with H1N1. Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation

and immunological cells were harvested after cytokines stimulation. Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line (16HBE). The optimal concentration of AD was defined by methyl-thiazolyl-tetrazolium (MTT) assay. After the virus infected cell model was established

AD was added into the medium as a treatment intervention. After 24-h co-culture

cell supernatant was collected for interferon gamma (IFN-γ) and interleukin-4 (IL-4) enzyme-linked immunosorbent assay (ELISA) detection while immunological cells for real-time polymerase chain reaction (RT-PCR). The optimal concentration of AD for anti-virus effect was 250 μg/mL. IL-4 and IFN-γ in the supernatant and mRNA levels in RLRs pathway increased when cells was infected by virus

RIG-I

IFN-β promoter stimulator-1 (IPS-1)

interferon regulatory factor (IRF)-7

IRF-3 and nuclear transcription factor κB (NF-κB) mRNA levels increased significantly (P<0.05). When AD was added into co-culture medium

the levels of IL-4 and IFN-γ were lower than those in the non-interference groups and the mRNA expression levels decreased

RIG-I

IPS-1

IRF-7

IRF-3 and NF-κB decreased significantly in each group with significant statistic differences (P<0.05). The RLRs mediated viral recognition provided a potential molecular target for acute viral infections and andrographolide could ameliorate H1N1 virus-induced cell mortality. And the antiviral effects might be related to its inhibition of viral-induced activation of the RLRs signaling pathway.

Abstract

AD was added into the medium as a treatment intervention. After 24-h co-culture

RIG-I

IFN-β promoter stimulator-1 (IPS-1)

interferon regulatory factor (IRF)-7

IRF-3 and nuclear transcription factor κB (NF-κB) mRNA levels increased significantly (P<0.05). When AD was added into co-culture medium

the levels of IL-4 and IFN-γ were lower than those in the non-interference groups and the mRNA expression levels decreased

RIG-I

IPS-1

IRF-7

关键词

andrographolideH1N1dendritic cellsretinoic acid-inducible gene-Iinterferon-β promoter stimulator-1interferon regulatory factornuclear transcription factor κB

Keywords

andrographolideH1N1dendritic cellsretinoic acid-inducible gene-Iinterferon-β promoter stimulator-1interferon regulatory factornuclear transcription factor κB

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