Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia

Chun-yu Guo; Xiao-juan Ma; Jing-shang Wang; Ying Shi; Xin Liu; Hui-jun Yin; Ke-ji Chen

doi:10.1007/s11655-014-1881-8

Your Location：

Home >

Browse articles >

Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia

Updated：2021-08-27

- Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia
- Chinese Journal of Integrative Medicine Vol. 21, Issue 5, Pages: 384-388(2015)
- Affiliations：
  
  Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the China Postdoctoral Science Fondation (No. 20100480427) and the National Natural Science Fondation of China (No. 81041038)
- DOI：10.1007/s11655-014-1881-8
  CLC：
- Published：2015，
  
  Published Online：14 July 2014，
Scan for full text
Guo, Cy., Ma, Xj., Wang, Js. et al. Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia., Chin. J. Integr. Med. 21, 384–388 (2015). https://doi.org/10.1007/s11655-014-1881-8

Chun-yu Guo, Xiao-juan Ma, Jing-shang Wang, et al. Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia. [J]. Chinese Journal of Integrative Medicine 21(5):384-388(2015)
Guo, Cy., Ma, Xj., Wang, Js. et al. Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia., Chin. J. Integr. Med. 21, 384–388 (2015). https://doi.org/10.1007/s11655-014-1881-8 DOI：

Chun-yu Guo, Xiao-juan Ma, Jing-shang Wang, et al. Effects of Panax Quinquefolium Saponin on phosphatidylinositol 3-kinase/serine threonine kinase pathway of neonatal rat myocardial cells subjected to hypoxia. [J]. Chinese Journal of Integrative Medicine 21(5):384-388(2015) DOI： 10.1007/s11655-014-1881-8.

摘要

To explore the effects of Panax Quinquefolium Saponin (PQS) on phosphatidylinositol 3-kinase/serine threonine kinase (PI3K/Akt) pathway of neonatal rat myocardial cells subjected to hypoxia. Neonatal rat myocardial cells were cultured in vitro. After the myocardial cell injury was induced by hypoxia

the cells were randomized into 5 groups: the normal group

the model group

the positive control group (Ciclosporin A

2 µ mol/L)

the low-dose PQS group (PQSL

25mg/L)

and the high-dose PQS group (PQSH

50 mg/L). Morphology and behavior of myocardial cells were observed under an inverted microscope. Apoptosis rate and lactate dehydrogenase (LDH) leakage rate of myocardial cells were determined by colorimetry. Mitochondrial transmembrane potential was assessed using a fluorexon laser. Phospho-glycogen synthase kinase (GSK)-3β and phospho-Akt as well as cytochrome C were determined by Western blot LDH leakage in the Ciclosporin A group

PQSH group and PQSL group reduced progressively compared with the model group (P<0.05). Akt and GSK-3β was strongly phosphorylated after treatment with Ciclosporin A and PQS compared with the model group (P<0.05

P<0.01). Compared with the model group (16.41±1.74; 35.28±6.30)

both the integrated optical density of mitochondrial permeability transition pore (MPTP) and the mitochondrial transmembrane potential significantly increased in the PQSH group (42.74±2.12; 71.36±6.54) and the PQSL group (39.58±1.49; 66.99±5.45; P<0.05

P<0.01). However

the protein of cytochrome C outside the mitochondrion decreased in the PQSH group (273.66±14.61) and the PQSL group (259.62±17.31) compared with the model group (502.41±17.76; P<0.05). Through activation of the PI3K/Akt pathway and inhibition of the MPTP

PQS might protect the heart against ischemia injury and apoptosis of myocardial cells.

Abstract

the cells were randomized into 5 groups: the normal group

the model group

the positive control group (Ciclosporin A

2 µ mol/L)

the low-dose PQS group (PQSL

25mg/L)

and the high-dose PQS group (PQSH

P<0.01). Compared with the model group (16.41±1.74; 35.28±6.30)

P<0.01). However

PQS might protect the heart against ischemia injury and apoptosis of myocardial cells.

关键词

Panax Quinquefolium Saponinischemic cardiomyocytesphosphatidylinositol 3-kinase/serinethreonine kinasemitochondrial permeability transition pore

Keywords

Panax Quinquefolium Saponinischemic cardiomyocytesphosphatidylinositol 3-kinase/serinethreonine kinasemitochondrial permeability transition pore

references

Guo CY, Yin HJ, Jiang YR, Xue M, Shi DZ. Differential gene expression profile in ischemic myocardium of Wistar rats with acute myocardial infarction. Chin Sci Bull (Chin) 2008;53:2488–2495.

Yin HJ, Guo CY, Shi DZ. Effects of Chinese herbs capable of replenishing qi, nourishing yin and activating blood circulation and their compatibility on differentially expressed genes of ischemic myocardium. Chin Sci Bull (Chin) 2009;54:3278–3282.

Javadov S, Baetz D, Rajapurohitam V, Zeidan A, Kirshenbaum LA, Karmazyn M. Antihypertrophic effect of Na+/H+ exchanger isoform 1 inhibition is mediated by reduced mitogen-activated protein kinase activation secondary to improved mitochondrial integrity and decreased generation of mitochondrial-derived reactive oxygen species. J Pharmacol Exp Ther 2006;317:1036–1043.

Hu DY. Updating concepts, converting modes to respond for new challenges of cardiovascular disease trends. Chin J Med (Chin) 2006;41:2–3.

Siragusa M, Katare R, Meloni M, Damilano F, Hirsch E, Emanueli C, et al. Involvement of phosphoinositide 3-kinase gamma in angiogenesis and healing of experimental myocardial infarction in mice. Circ Res 2010;106:757–768.

Downey JM, Cohen MV. Reducing infarct size in the setting of acute myocardial infarction. Prog Cardiovasc Dis 2006;48:363–371.

Hiller S, Garces RG, Malia TJ, Orekhov VY, Colombini M, Wagner G. Solution structure of the integral human membrane protein VDAC-1 in detergent micelles. Science 2008;321:1206–1210.

Piot C. Effect of cyclosporine on reperfusion injury in acute myocardial infarction. N Engl J Med 2008;359:473–481.

Bulhak AA, Jung C, Ostenson CG, Lundberg JO, Sjöquist PO, Pernow J. PPAR-alpha activation protects the type 2 diabetic myocardium against ischemia-reperfusion injury: involvement of the PI3-kinase/Akt and NO pathway. Am J Physiol Heart Circ Physiol 2009;296:H719–727.

Yin HJ, Zhang Y, Jiang YR, Luo L, Liu Y, Shi DZ, Chen KJ. Effect of folium Panax Quinquefolium Saponins on apoptosis of cardiac muscle cells and apoptosis-related gene expression in rats with acute myocardial infarction. Chin J Integr Tradit West Med (Chin) 2005;25:232–235.

Yin HJ, Zhang Y, Yang LH, Bai GR, Shi DZ, Chen KJ. The effects of PQS on glucose transport, GLUT4 translocation and CAP mRNA expression of adipocytes. Chin Pharmacol Bull (Chin) 2007;23:1332–1337.

FULL TEXT LINK

More>

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

Address：1 Caochang, xiyuan, Beijing, People's Republic of China Postal code：100091
Tel：010-62886827, 62877592 Fax：010-62874291
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05067934号-1 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰