Protective effect of emodin against airway inflammation in the ovalbumin-induced mouse model

Tan Wang; Xiang-gen Zhong; Yu-hang Li; Xu Jia; Shu-jing Zhang; Yu-shan Gao; Miao Liu; Ruo-han Wu

doi:10.1007/s11655-014-1898-z

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Protective effect of emodin against airway inflammation in the ovalbumin-induced mouse model

Updated：2021-08-27

- Protective effect of emodin against airway inflammation in the ovalbumin-induced mouse model
- Chinese Journal of Integrative Medicine Vol. 21, Issue 6, Pages: 431-437(2015)
- Affiliations：
  
  School of Basic Medical Sciences, Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Program on Key Basic Research Program Project (973 Program, No. 2009CB522704)
- DOI：10.1007/s11655-014-1898-z
  CLC：
- Published：2015，
  
  Published Online：18 December 2014，
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Wang, T., Zhong, Xg., Li, Yh. et al. Protective effect of emodin against airway inflammation in the ovalbumin-induced mouse model., Chin. J. Integr. Med. 21, 431–437 (2015). https://doi.org/10.1007/s11655-014-1898-z

Tan Wang, Xiang-gen Zhong, Yu-hang Li, et al. Protective effect of emodin against airway inflammation in the ovalbumin-induced mouse model. [J]. Chinese Journal of Integrative Medicine 21(6):431-437(2015)
Wang, T., Zhong, Xg., Li, Yh. et al. Protective effect of emodin against airway inflammation in the ovalbumin-induced mouse model., Chin. J. Integr. Med. 21, 431–437 (2015). https://doi.org/10.1007/s11655-014-1898-z DOI：

Tan Wang, Xiang-gen Zhong, Yu-hang Li, et al. Protective effect of emodin against airway inflammation in the ovalbumin-induced mouse model. [J]. Chinese Journal of Integrative Medicine 21(6):431-437(2015) DOI： 10.1007/s11655-014-1898-z.

摘要

To investigate whether emodin exerts protective effects on mouse with allergic asthma. A mouse model of allergic airway inflflammation was employed. The C57BL/6 mice sensitized and challenged with ovalbumin (OVA) were intraperitoneally administered 10 or 20 mg/kg emodin for 3 days during OVA challenge. Animals were sacrificed 48 h after the last challenge. Inflammatory cell count in the bronchoalveolar lavage fluid (BALF) was measured. The levels of interleukin (IL)-4

IL-5

IL-13 and eotaxin in BALF and level of immunoglobulin E (IgE) in serum were measured with enzyme-linked immuno sorbent assay kits. The mRNA expressions of IL-4

IL-5

heme oxygenase (HO)-1 and matrix metalloproteinase-9 (MMP-9) were determined by real-time quantitative polymerase chain reaction. Emodin induced significant suppression of the number of OVA-induced total inflammatory cells in BALF. Treatment with emodin led to significant decreases in the levels of IL-4

IL-5

IL-13 and eotaxin in BALF and total IgE level in serum. Histological examination of lung tissue revealed marked attenuation of allergen-induced lung eosinophilic inflammation. Additionally

emodin suppressed IL-4

IL-5 and MMP-9 mRNA expressions and induced HO-1 mRNA expression. Emodin exhibits anti-inflammatory activity in the airway inflammation mouse model

supporting its therapeutic potential for the treatment of allergic bronchial asthma.

Abstract

IL-5

IL-13 and eotaxin in BALF and level of immunoglobulin E (IgE) in serum were measured with enzyme-linked immuno sorbent assay kits. The mRNA expressions of IL-4

IL-5

IL-13 and eotaxin in BALF and total IgE level in serum. Histological examination of lung tissue revealed marked attenuation of allergen-induced lung eosinophilic inflammation. Additionally

emodin suppressed IL-4

IL-5 and MMP-9 mRNA expressions and induced HO-1 mRNA expression. Emodin exhibits anti-inflammatory activity in the airway inflammation mouse model

supporting its therapeutic potential for the treatment of allergic bronchial asthma.

关键词

AsthmaEmodinairway inflammationChinese Medicine

Keywords

AsthmaEmodinairway inflammationChinese Medicine

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