Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells

Lu Ma; Xiao-fa Zhu; Yu-yun Wu; Ke-ji Chen; Da-zhuo Shi; Hui-jun Yin

doi:10.1007/s11655-014-1980-6

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Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells

Updated：2021-08-27

- Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells
- Chinese Journal of Integrative Medicine Vol. 21, Issue 4, Pages: 299-306(2015)
- Affiliations：
  
  1. Department of Geriatric Cardiology, the Navy General Hospital of Chinese PLA,Beijing,China
  2. The Lab of Cardiovascular Molecular Biology, Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-014-1980-6
  CLC：
- Published：2015，
  
  Published Online：14 January 2015，
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Ma, L., Zhu, Xf., Wu, Yy. et al. Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells., Chin. J. Integr. Med. 21, 299–306 (2015). https://doi.org/10.1007/s11655-014-1980-6

Lu Ma, Xiao-fa Zhu, Yu-yun Wu, et al. Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells. [J]. Chinese Journal of Integrative Medicine 21(4):299-306(2015)
Ma, L., Zhu, Xf., Wu, Yy. et al. Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells., Chin. J. Integr. Med. 21, 299–306 (2015). https://doi.org/10.1007/s11655-014-1980-6 DOI：

Lu Ma, Xiao-fa Zhu, Yu-yun Wu, et al. Protective effect of propyl gallate against oxidized low-density lipoprotein-induced injury of endothelial cells. [J]. Chinese Journal of Integrative Medicine 21(4):299-306(2015) DOI： 10.1007/s11655-014-1980-6.

摘要

To evaluate the protective effect of propyl gallate (PG)

an alkyl ester of gallic acid which is an active ingredient of Radix Paeoniae

against oxidized low-density lipoprotein (ox-LDL)-induced apoptosis and death in endothelial cells (ECs) and to find out its preliminary mechanism. The cultured endothelial cells were divided into normal

model (ox-LDL)

control (fetal bovine serum)

PG high dose (20 μg/mL)

PG middle dose (10 μg/mL)

and PG low dose (5 μg/mL) groups

each derived from three different pools of umbilical cords. The model of injured human umbilical vein endothelial cells (HUVECs) was induced by ox-LDL. The 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyl-2H-tetrazolium bromide (MTT) assay

Hoechst 33258 staining

flow cytometry and measurement of nitrogen monoxidum (NO) release were used to evaluate the protective effect of PG against ox-LDL-induced apoptosis and death in HUVECs. To find out the mechanism of this protective effect

the expression of endothelial nitric oxide synthase (eNOS) mRNA

eNOS protein expression

immunofluorescence of intracellular reactive oxygen species (ROS) and activities of malondialdehyde (MDA)

superoxidedismutase (SOD) and glutathione peroxidase (GPx) were observed. PG significantly reduced ox-LDL-induced apoptosis and cell death. The percentage of cells death and apoptosis was significantly higher in the ox-LDL group than that in the control group (P<0.05). Compared with the control group

the cells death and apoptosis of PG group was no different (P>0.05). As compared with the ox-LDL group

results of the PG high dose group showed that cell viability was significantly increased (P<0.05)

the level of NO release

expression of eNOS mRNA

densitometric value of eNOS protein expression

as well as the activities of SOD and GPx were all significantly higher (all P<0.05). PG could potentially serve as a novel endothelial protective agent against ox-LDL-induced injury of endothelial cell.

Abstract

To evaluate the protective effect of propyl gallate (PG)

an alkyl ester of gallic acid which is an active ingredient of Radix Paeoniae

model (ox-LDL)

control (fetal bovine serum)

PG high dose (20 μg/mL)

PG middle dose (10 μg/mL)

and PG low dose (5 μg/mL) groups

each derived from three different pools of umbilical cords. The model of injured human umbilical vein endothelial cells (HUVECs) was induced by ox-LDL. The 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyl-2H-tetrazolium bromide (MTT) assay

Hoechst 33258 staining

the expression of endothelial nitric oxide synthase (eNOS) mRNA

eNOS protein expression

immunofluorescence of intracellular reactive oxygen species (ROS) and activities of malondialdehyde (MDA)

the cells death and apoptosis of PG group was no different (P>0.05). As compared with the ox-LDL group

results of the PG high dose group showed that cell viability was significantly increased (P<0.05)

the level of NO release

expression of eNOS mRNA

densitometric value of eNOS protein expression

关键词

propyl gallateoxidized low-density lipoproteinendotheliocytereactive oxygen speciesRadix PaeoniaeChinese Medicine

Keywords

propyl gallateoxidized low-density lipoproteinendotheliocytereactive oxygen speciesRadix PaeoniaeChinese Medicine

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