Qiangzhi Decoction (羌跖汤) protects mice from influenza A pneumonia through inhibition of inflammatory cytokine storm

Hai-yan Zhu; Hai Huang; Xun-long Shi; Wei Zhou; Pei Zhou; Qian-lin Yan; Hong-guang Zhu; Dian-wen Ju

doi:10.1007/s11655-014-2020-2

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Qiangzhi Decoction (羌跖汤) protects mice from influenza A pneumonia through inhibition of inflammatory cytokine storm

Updated：2021-08-27

- Qiangzhi Decoction (羌跖汤) protects mice from influenza A pneumonia through inhibition of inflammatory cytokine storm
- Chinese Journal of Integrative Medicine Vol. 21, Issue 5, Pages: 376-383(2015)
- Affiliations：
  
  1. Department of Biosynthesis, School of Pharmacy, Fudan University,Shanghai,China
  2. Institute of Chinese Medicine, Tongji University,Shanghai,China
  3. Shanghai Medical College, Fudan University,Shanghai,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (NO.81173173), and Shanghai Yan De-xin Foundation for Traditional Chinese Medicine
- DOI：10.1007/s11655-014-2020-2
  CLC：
- Published：2015，
  
  Published Online：17 December 2014，
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Zhu, Hy., Huang, H., Shi, Xl. et al. Qiangzhi Decoction (羌跖汤) protects mice from influenza A pneumonia through inhibition of inflammatory cytokine storm., Chin. J. Integr. Med. 21, 376–383 (2015). https://doi.org/10.1007/s11655-014-2020-2

Hai-yan Zhu, Hai Huang, Xun-long Shi, et al. Qiangzhi Decoction (羌跖汤) protects mice from influenza A pneumonia through inhibition of inflammatory cytokine storm. [J]. Chinese Journal of Integrative Medicine 21(5):376-383(2015)
Zhu, Hy., Huang, H., Shi, Xl. et al. Qiangzhi Decoction (羌跖汤) protects mice from influenza A pneumonia through inhibition of inflammatory cytokine storm., Chin. J. Integr. Med. 21, 376–383 (2015). https://doi.org/10.1007/s11655-014-2020-2 DOI：

Hai-yan Zhu, Hai Huang, Xun-long Shi, et al. Qiangzhi Decoction (羌跖汤) protects mice from influenza A pneumonia through inhibition of inflammatory cytokine storm. [J]. Chinese Journal of Integrative Medicine 21(5):376-383(2015) DOI： 10.1007/s11655-014-2020-2.

摘要

To investigate the preventive effects of Qiangzhi Decoction (羌跖汤

QZD) on influenza A pneumonia through inhibition of inflammatory cytokine storm in vivo and in vitro. One hundred ICR mice were randomly divided into the virus control

the Tamiflu control and the QZD high-

medium-

and low-dose groups. Mice were infected intranasally with influenza virus (H1N1) at 10 median lethal dose (LD50). QZD and Tamiflu were administered intragastrically twice daily from day 0 to day 7 after infection. The virus control group was treated with distilled water alone under the same condition. The number of surviving mice was recorded daily for 14 days after viral infection. The histological damage and viral replication and the expression of inflammatory cytokines were monitored. Additionally

the suppression capacity on the secretion of regulated on activation normal T cells expressed and secreted (RANTES) and tumor necrosis factor-α (TNF-α) in epithelial and macrophage cell-lines were evaluated. Compared with the virus control group

the survival rate of the QZD groups signifificantly improved in a dose-dependent manner (P<0.05)

the viral titers in lung tissue was inhibited (P<0.05)

and the production of inflammatory cytokines interferon-γ (IFN-γ)

interleukin-6 (IL-6)

TNF-α

and intercellular adhesion molecule-1 (ICAM-1) were suppressed (P<0.05). Meanwhile

the secretion of RANTETS and TNF-α by epithelial and macrophage cell-lines was inhibited with the treatment of QZD respectively in vitro (p<0.05) The preventive effects of QZD on influenza virus infection might be due to its unique cytokine inhibition mechanism. QZD may have significant therapeutic potential in combination with antiviral drugs.

Abstract

To investigate the preventive effects of Qiangzhi Decoction (羌跖汤

QZD) on influenza A pneumonia through inhibition of inflammatory cytokine storm in vivo and in vitro. One hundred ICR mice were randomly divided into the virus control

the Tamiflu control and the QZD high-

medium-

the survival rate of the QZD groups signifificantly improved in a dose-dependent manner (P<0.05)

the viral titers in lung tissue was inhibited (P<0.05)

and the production of inflammatory cytokines interferon-γ (IFN-γ)

interleukin-6 (IL-6)

TNF-α

and intercellular adhesion molecule-1 (ICAM-1) were suppressed (P<0.05). Meanwhile

关键词

herbal medicineinfluenza A virusantiviral activitypneumoniainflammatory cytokineChinese Medicine

Keywords

herbal medicineinfluenza A virusantiviral activitypneumoniainflammatory cytokineChinese Medicine

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