Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury

Yi-min Zhang; Sheng-xin Chen; Qiu-fu Dai; Shu-ting Jiang; Ai-lian Chen; Chun-zhi Tang; Yu-qing Zhang

doi:10.1007/s11655-015-1969-9

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Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury

Updated：2021-08-27

- Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury
- Chinese Journal of Integrative Medicine Vol. 24, Issue 7, Pages: 537-544(2018)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, School of Medicine, Jinan University,Guangzhou,China
  2. Clinical Medical School of Acupuncture Rehabilitation, Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81273827), the Project of Science and Technology of Guangdong, China (No. 2011B031800284) and the Science and Technology Program of Guangzhou, China (No. 2010GN-E00221)
- DOI：10.1007/s11655-015-1969-9
  CLC：
- Published：2018，
  
  Published Online：24 April 2015，
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Zhang, Ym., Chen, Sx., Dai, Qf. et al. Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury., Chin. J. Integr. Med. 24, 537–544 (2018). https://doi.org/10.1007/s11655-015-1969-9

Yi-min Zhang, Sheng-xin Chen, Qiu-fu Dai, et al. Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury. [J]. Chinese Journal of Integrative Medicine 24(7):537-544(2018)
Zhang, Ym., Chen, Sx., Dai, Qf. et al. Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury., Chin. J. Integr. Med. 24, 537–544 (2018). https://doi.org/10.1007/s11655-015-1969-9 DOI：

Yi-min Zhang, Sheng-xin Chen, Qiu-fu Dai, et al. Effect of Acupuncture on the Notch Signaling Pathway in Rats with Brain Injury. [J]. Chinese Journal of Integrative Medicine 24(7):537-544(2018) DOI： 10.1007/s11655-015-1969-9.

摘要

To observe the effect of acupuncture on the Notch signaling pathway in rats with traumatic brain injury and to explore the pathogenesis of acupuncture intervention on traumatic brain injury. Feeney’s freefall epidural impact method was used to establish a traumatic brain injury model in rats; the rats were randomly divided into a normal group

sham operation group

model group and acupuncture group. Acupuncture was performed in the Baihui (DU 20)

Shuigou (DU 26)

Fengfu (DU 16)

Yamen (DU 15) and Hegu (LI 4) acupoints in the rat

and Yamen was punctured via Fengfu. Then

the rats in each group were randomly divided into three subgroups

namely the day 3 subgroup

day 7 subgroup and day 14 subgroup according to treatment duration. The modified neurological severity scores (mNss) method was used to perform neurobehavioral scoring for evaluating the degree of injury in the rats. The hematoxylin-eosin (HE) staining method was used to observe the pathological change in the brain tissue of rats in each group. Real-time fluorescent quantitative polymerase chain reaction (Q-PCR) technology was used to detect changes in the Notch1

Hes1 and Hes5 gene expression levels in the cortex on the injured side. Western blot was used to detect the protein expression changes. One day after modeling

the mNss scores in the model group and in the acupuncture group were significantly higher than those in the normal and sham operation groups (P<0.01) ; there was no statistically significant difference between the normal group and the sham operation group. The scores decreased with increased treatment time

and the scores in the acupuncture group decreased more significantly than those in the model group (P<0.01). The pathological examination by the HE staining method demonstrated that the brain tissue of the rats in the acupuncture and model groups relatively significantly changed. The Notch1 gene expression level in the acupuncture group was significantly higher than the level in all of the other groups (P<0.01) ; the Hes1 and Hes5 gene expression levels were also higher in the acupuncture group. The expression changes of the Notch1 and Hes1 protein were consistent with that of mRNA. In each experimental group

the mNss score and the pathological results by the HE staining method were consistent with the mRNA results. Acupuncture could significantly promote high expression levels of Notch1

Hes1 and Hes5 in the brain tissue of traumatic brain injury rats. Therefore

acupuncture might be an important intervention for inducing endogenous stem cell proliferation and for promoting nerve repair.

Abstract

sham operation group

model group and acupuncture group. Acupuncture was performed in the Baihui (DU 20)

Shuigou (DU 26)

Fengfu (DU 16)

Yamen (DU 15) and Hegu (LI 4) acupoints in the rat

and Yamen was punctured via Fengfu. Then

the rats in each group were randomly divided into three subgroups

namely the day 3 subgroup

Hes1 and Hes5 gene expression levels in the cortex on the injured side. Western blot was used to detect the protein expression changes. One day after modeling

the mNss score and the pathological results by the HE staining method were consistent with the mRNA results. Acupuncture could significantly promote high expression levels of Notch1

Hes1 and Hes5 in the brain tissue of traumatic brain injury rats. Therefore

acupuncture might be an important intervention for inducing endogenous stem cell proliferation and for promoting nerve repair.

关键词

brain injuryNotchHes1Hes5acupuncture

Keywords

brain injuryNotchHes1Hes5acupuncture

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Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University

The First Hospital Affiliated to Tianjin University of Traditional Chinese Medicine

The Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine

Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences

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