Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes

Wei-wei Wen; Xin Sun; Hai-feng Zhuang; Xiao-jie Lin; Zhi-yin Zheng; Rui-lan Gao; Li-ming Yin

doi:10.1007/s11655-015-1970-3

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Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes

Updated：2021-08-27

- Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes
- Chinese Journal of Integrative Medicine Vol. 22, Issue 1, Pages: 28-35(2016)
- Affiliations：
  
  1. The Third People’s Hospital of Hangzhou,Hangzhou,China
  2. Department of Oncology, Zhejiang Provincial People’s Hospital,Hangzhou,China
  3. Institution of Hematology Research, the First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81373876), Zhejiang Provincial Natural Science Foundation (No. LY14H290004) and Science and Technology Program of Zhejiang Province (No. 2010C33098)
- DOI：10.1007/s11655-015-1970-3
  CLC：
- Published：2016，
  
  Published Online：24 April 2015，
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Wen, Ww., Sun, X., Zhuang, Hf. et al. Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes., Chin. J. Integr. Med. 22, 28–35 (2016). https://doi.org/10.1007/s11655-015-1970-3

Wei-wei Wen, Xin Sun, Hai-feng Zhuang, et al. Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes. [J]. Chinese Journal of Integrative Medicine 22(1):28-35(2016)
Wen, Ww., Sun, X., Zhuang, Hf. et al. Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes., Chin. J. Integr. Med. 22, 28–35 (2016). https://doi.org/10.1007/s11655-015-1970-3 DOI：

Wei-wei Wen, Xin Sun, Hai-feng Zhuang, et al. Effects of panaxadiol saponins component as a new Chinese patent medicine on proliferation, differentiation and corresponding gene expression profile of megakaryocytes. [J]. Chinese Journal of Integrative Medicine 22(1):28-35(2016) DOI： 10.1007/s11655-015-1970-3.

摘要

To investigate the effects of panaxadiol saponins component (PDS-C) isolated from total saponins of panax ginseng on proliferation

differentiation and corresponding gene expression profile of megakaryocytes. Bone marrow culture of colony forming assay of megakaryocytic progenitor cells (CFU-MK) was observed for the promoting proliferation mediated by PDS-C

and differentiation of megakaryocytic blasts caused by PDS-C was analyzed with flow cytometry in CHRF-288 and Meg-01 cells

as well as proliferation

differentiation-related genes expression profile and protein expression levels were detected by human gene expression microarray and western blot. In response to PDS-C 10

20 and 50 mg/L

CFU-MK from 10 human bone marrow samples was increased by 28.9%±2.7%

41.0%±3.2% and 40.5%±2.6% over untreated control

respectively (P <0.01

each). Flow cytometry analysis showed that PDS-C treated CHRF-288 cells and Meg-01 cells significantly increased in CD42b

CD41

TSP and CD36 positive ratio

respectively. PDS-C induced 29 genes up-regulated more than two-fold commonly in both cells detected by human expression microarray representing 4000 known genes. The protein expression levels of ZNF91

c-Fos

BTF3a

GATA-1

RGS2

NDRG2 and RUNX1 were increased with western blot in correspond to microarray results. PDS-C as an effective component for hematopoiesis

play the role to enhance proliferation and differentiation of megakaryocytes

also up-regulated expression of proliferation

differentiation-related genes and proteins in vitro.

Abstract

To investigate the effects of panaxadiol saponins component (PDS-C) isolated from total saponins of panax ginseng on proliferation

and differentiation of megakaryocytic blasts caused by PDS-C was analyzed with flow cytometry in CHRF-288 and Meg-01 cells

as well as proliferation

differentiation-related genes expression profile and protein expression levels were detected by human gene expression microarray and western blot. In response to PDS-C 10

20 and 50 mg/L

CFU-MK from 10 human bone marrow samples was increased by 28.9%±2.7%

41.0%±3.2% and 40.5%±2.6% over untreated control

respectively (P <0.01

each). Flow cytometry analysis showed that PDS-C treated CHRF-288 cells and Meg-01 cells significantly increased in CD42b

CD41

TSP and CD36 positive ratio

c-Fos

BTF3a

GATA-1

RGS2

NDRG2 and RUNX1 were increased with western blot in correspond to microarray results. PDS-C as an effective component for hematopoiesis

play the role to enhance proliferation and differentiation of megakaryocytes

also up-regulated expression of proliferation

differentiation-related genes and proteins in vitro.

关键词

panaxadiol saponinsmegakaryocytegene expression profileproliferationdifferentiation

Keywords

panaxadiol saponinsmegakaryocytegene expression profileproliferationdifferentiation

references

Gao RL, Xu CL, Jin JM. Effect of total saponins of panax ginseng on hematopoietic progenitor cells in normal human and aplastic anemia patients. Chin J Integr Tradit West Med (Chin) 1992;12:285–287.

Chen XH, Gao RL, Xu WH. Effect of ginsenosides in inducing proliferation and transcription factor of erythrocytic, granulo-monocytic and megakarocytic cell lines. Chin J Integr Tradit West Med (Chin) 2001;21:40–42.

Jin J, Tao H, Gao R. Effect of ginsenosides on proliferation and differentiation of human CD34+ hematopoietic stem/progenitor cells. Chin J Integr Tradit West Med (Chin) 2000;20:673–676.

Fang GL, Gao RL, Lin XJ, Jin JM. Effects of ginseng panaxadiol saponin on proliferation and differentiation of human bone marrow CD34+ cells. J Exp Hematol (Chin) 2007;15:776–779.

Gao RL, Chen XH, Lin XJ, Qian XD, Xu WH, Chong BH. Effects of notoginosides on proliferation and up-regulation of GRnuclear tanscription factor in hematopoietic cells. Acta Pharmacol Sin 2007;28:703–707.

Schena M, Shalon D, Dais RW, Brown PO. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 1995; 270(20):467–470.

Sun X, Gao RL, Lin XJ, Xu WH, Chen XH. Panax notoginseng saponins induced up-regulation, phosphorylation and binding activity of MEK, ERK, AKT, PI-3K protein kinases and GATA transcription factors in hematopoietic cells. Chin J Integr Med 2013;19:112–118.

Zuo G, Guan T, Chen D, Li C, Jiang R, Luo C, et al. Total saponins of Panax ginseng induces K562 cell differentiation by promoting internalization of the erythropoietin receptor. Am J Chin Med 2009;37:747–757.

Wang JW, Wang YP, Wang SL, Jiang R. Synergistic effects of total saponins of Panax ginseng in combination with hematopoietic growth factor on proliferation and differentiation of CD34(+) cells ex vivo. J Exp Hematol (Chin) 2006;14:959–963.

Gao RL, Chong BH. Research and development of the effective components of panaxadiol saponin as new Chinese patent medicine for treating hemocytopenia. Chin J Integr Med 2012;18:892–902.

Goldfarb AN. Megakaryocytic programming by a transciptional regulatory loop: a circle connecting RUNX1, GATA-1, and P-TEFb. J Cell Biochem 2009;107:377–382.

Krumsiek J, Marr C, Timm Schroeder T, Theis FJ. Hierarchical differentiation of myeloid progenitors is encoded in the transcription factor network. Plos One 2011;6:e22649.

Elagib KE, Mihaylov IS, Delephanty LL, Bullock GC, Ouma KD, Caronia JF, et al. Cross-talk of GATA-1 and P-TEFb in megakaryocyte differentiation. Blood 2008;112:4884–4894.

Unoki M, Okutsu J, Nakamura Y. Identification of a novel human gene, ZFP91, involved in acute myelogenous leukemia. Int J Oncol 2003;22:1217–1223.

Li R, Liu XL, Du QF, Zhang S, Luo RC, Zhou SY. Proteome analysis of apoptotic K562 cells induced by harringtonine. Chin J Hematol (Chin) 2004;25:323–327.

Kang K, Jung H, Nam S, Lim JS. NDRG2 promotes GATA-1 expression through regulation of the JAK2/STAT pathway in PMA-stimulated U937 cells. Immune Netw 2011;11:348–357.

Schwäble J, Choudhary C, Thiede C, Tickenbrock L, Sargin B, Steur C, et al. RGS2 is an important target gene of Flt3-ITD mutations in AML and functions in myeloid differentiation and leukemic transformation. Blood 2005;105:2107–2114.

Tijssen MR, Cvejic A, Joshi A, Hannah RL, Ferreira R, Forrai A, et al. Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators. Dev Cell 2011;20:597–609.

Gao R, Wu C, Chong BH. The intracellular signaling pathway initiated by ginsenosides in hematopoietic cells. Chin J Hematol (Chin) 1999;20:292–295.

Gao RL, Xu WH, Chen XH, Qian XD, Wu CQ. Up-regulation of transcription factors NF-E2, c-jun and c-fos of AP-1 family induced by panax notoginosides in hematopoietic cells. J Exp Hematol (Chin) 2004; 12:16–19.

Gao RL, Xu WH, Lin XJ, Chen XH, Wu CQ. Up-regulation of transcription factors GATA-1 and GATA-2 induced by Panax notoginosides in hematopoietic cells. Chin J Hematol (Chin) 2004;25:281–284.

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