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Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B
- Chinese Journal of Integrative Medicine Vol. 22, Issue 6, Pages: 457-466(2016)
- Affiliations：
1. Traditional Chinese Medicine Department, Qingdao Women and Children Hospital,Shandong Province,Qingdao,China
2. College of Acupuncture and Moxibustion and Massage, Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：Supported by the 45th China Postdoctoral Science Foundation (No. 20090450856)
DOI：10.1007/s11655-015-1972-1
CLC：Published：2016，
Published Online：12 May 2015，
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Xu, T., Xu, Ng., Yang, Zh. et al. Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B., Chin. J. Integr. Med. 22, 457–466 (2016). https://doi.org/10.1007/s11655-015-1972-1
Tao Xu, Neng-gui Xu, Zhong-hua Yang, et al. Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B. [J]. Chinese Journal of Integrative Medicine 22(6):457-466(2016)
Xu, T., Xu, Ng., Yang, Zh. et al. Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B., Chin. J. Integr. Med. 22, 457–466 (2016). https://doi.org/10.1007/s11655-015-1972-1 DOI：
Tao Xu, Neng-gui Xu, Zhong-hua Yang, et al. Neuroprotective effects of electroacupuncture on hypoxic-ischemic encephalopathy in newborn rats are associated with increased expression of GDNF-RET and protein kinase B. [J]. Chinese Journal of Integrative Medicine 22(6):457-466(2016) DOI： 10.1007/s11655-015-1972-1.
摘要
To explore the neuroprotective effects of electroacupuncture (EA) on hypoxic-ischemic encephalopathy (HIE) and to further investigate the role of glial cell line-derived neurotrophic factor (GDNF) family receptor member RET (rearranged during transfection) and its key downstream phosphatidylinositol 3 kinase (PI-3K)/protein kinase B (Akt) pathway in the process. A total of 220 seven-day-old SD rats (of either sex
from 22 broods) were randomly divided into two groups
one (30 rats) for sham-surgery group and the other (190 rats) for HIE model group. The HIE model was established using the left common carotid artery ligation method in combination with hypoxic treatment. The successfully established rats were randomly divided into five groups
including control model group
EA group
sham-EA group
antagonist group and antagonist plus electroacupuncture group
with 35 rats in each group. Baihui (GV 20)
Dazhui (GV 14)
Quchi (LI 11) and Yongquan (KI 1) acupoints were chosen for acupuncture. EA was performed at Baihui and Quchi for 10 min once a day for continuous 1
3
7 and 21 days
respectively. The rats were then killed after the operation and injured cerebral cortex was taken for the measurement of neurologic damage by hematoxylin-eosin (HE) staining and the degenerative changes of cortical ultrastructure by transmission electron microscopy. RET mRNA level and Akt protein level were detected by real-time reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis
respectively. EA could ameliorate neurologic damage of the first somatic sensory area (S1Tr) and alleviate the degenerative changes of ultrastructure of cortical neurons in rats subjected to HIE. And the longer acupuncture treatment lasted
the better its therapeutic effect would be. This was accompanied by gradually increased expression of GDNF family receptor RET at the mRNA level and its downstream signaling Akt at the protein level in the ischemic cortex. EA has neuroprotective effects on HIE and could be a potential therapeutic strategy for HIE in the neonate. Activation of RET/Akt signaling pathway might be involved in this process.
Abstract
To explore the neuroprotective effects of electroacupuncture (EA) on hypoxic-ischemic encephalopathy (HIE) and to further investigate the role of glial cell line-derived neurotrophic factor (GDNF) family receptor member RET (rearranged during transfection) and its key downstream phosphatidylinositol 3 kinase (PI-3K)/protein kinase B (Akt) pathway in the process. A total of 220 seven-day-old SD rats (of either sex
from 22 broods) were randomly divided into two groups
one (30 rats) for sham-surgery group and the other (190 rats) for HIE model group. The HIE model was established using the left common carotid artery ligation method in combination with hypoxic treatment. The successfully established rats were randomly divided into five groups
including control model group
EA group
sham-EA group
antagonist group and antagonist plus electroacupuncture group
with 35 rats in each group. Baihui (GV 20)
Dazhui (GV 14)
Quchi (LI 11) and Yongquan (KI 1) acupoints were chosen for acupuncture. EA was performed at Baihui and Quchi for 10 min once a day for continuous 1
3
7 and 21 days
respectively. The rats were then killed after the operation and injured cerebral cortex was taken for the measurement of neurologic damage by hematoxylin-eosin (HE) staining and the degenerative changes of cortical ultrastructure by transmission electron microscopy. RET mRNA level and Akt protein level were detected by real-time reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis
respectively. EA could ameliorate neurologic damage of the first somatic sensory area (S1Tr) and alleviate the degenerative changes of ultrastructure of cortical neurons in rats subjected to HIE. And the longer acupuncture treatment lasted
the better its therapeutic effect would be. This was accompanied by gradually increased expression of GDNF family receptor RET at the mRNA level and its downstream signaling Akt at the protein level in the ischemic cortex. EA has neuroprotective effects on HIE and could be a potential therapeutic strategy for HIE in the neonate. Activation of RET/Akt signaling pathway might be involved in this process.
关键词
hypoxic-ischemic encephalopathyelectroacupunctureglial cell line-derived neurotrophic factorrearranged during transfectionprotein kinase B
Keywords
hypoxic-ischemic encephalopathyelectroacupunctureglial cell line-derived neurotrophic factorrearranged during transfectionprotein kinase B
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