Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells

Yu-hong Zhou; Jin-yi Feng; Liang-shun You; Hai-tao Meng; Wen-bin Qian

doi:10.1007/s11655-015-1975-y

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Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells

Updated：2021-08-27

- Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells
- Chinese Journal of Integrative Medicine Vol. 21, Issue 8, Pages: 635-639(2015)
- Affiliations：
  
  1. The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
  2. Institute of Hematology, the First Affiliated Hospital, College of Medicine, Zhejiang University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30871099), Zhejiang Provincial Natural Science Foundation of China (No. R2090392), and Administration of Traditional Chinese Medicine of Zhejiang Province (No. 2000C25)
- DOI：10.1007/s11655-015-1975-y
  CLC：
- Published：2015，
  
  Published Online：24 March 2015，
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Zhou, Yh., Feng, Jy., You, Ls. et al. Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells., Chin. J. Integr. Med. 21, 635–639 (2015). https://doi.org/10.1007/s11655-015-1975-y

Yu-hong Zhou, Jin-yi Feng, Liang-shun You, et al. Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells. [J]. Chinese Journal of Integrative Medicine 21(8):635-639(2015)
Zhou, Yh., Feng, Jy., You, Ls. et al. Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells., Chin. J. Integr. Med. 21, 635–639 (2015). https://doi.org/10.1007/s11655-015-1975-y DOI：

Yu-hong Zhou, Jin-yi Feng, Liang-shun You, et al. Matrine and CYC116 synergistically inhibit growth and induce apoptosis in multiple myeloma cells. [J]. Chinese Journal of Integrative Medicine 21(8):635-639(2015) DOI： 10.1007/s11655-015-1975-y.

摘要

To investigate whether CYC116 can potentiate matrine-dependent growth inhibition and apoptosis in multiple myeloma (MM) cells. The dose response relationship of matrine to dexamethasone-resistant and dexamethasone-sensitive MM cells was first established. Myeloma RPMI8226 cells were treated with matrine alone or combined with CYC116 for 24 h. Cell proliferation was measured using an MTT assay and apoptosis induction was evaluated by flow cytometry. Activation of the caspase pathway and expression of apoptosis regulator proteins were detected by Western blotting. Matrine significantly induced growth arrest and apoptosis in both drug-resistant and drug-sensitive MM cells. Treatment with the combination of matrine and CYC116 had a stronger cytotoxic effect on MM cells than did single drug treatments. Enhanced apoptosis observed following the combined treatment of matrine and CYC116 was associated with higher levels of activation of caspase-9

caspase-3

and poly adenosine diphosphate ribose polymerase (PARP) and down-regulation of the anti-apoptotic proteins Bcl-2 and Mcl-1 and the signaling proteins p-Akt and nuclear factor κB (NF-κB). CYC116 enhances the growth inhibitory and apoptotic effects of matrine on MM cells.

Abstract

caspase-3

关键词

apoptosisaurora kinaseCYC116inhibitorMatrinemultiple myeloma

Keywords

apoptosisaurora kinaseCYC116inhibitorMatrinemultiple myeloma

references

Sunq B, Kunnumakkara AB, Sethi G, Anand P, Guhan S, Aqqarwal BB. Curcumin circumvents chemoresistance in vitro and potentiates the effect of thalidomide and bortezomib against human multiple myeloma in nude mice model. Mol Cancer Ther 2009;8:959-970.

Feng X, Tang HM, Shen LH. Overview of the research of matrine pharmacology. J Clin Epidemiol (Chin) 2005;14:331-333.

Thompson CB. Apoptosis in the pathogenesis and treatment of disease. Science 1995;267:1456-1462.

Hannum YA. Apoptosis and the dilemma of cancer chemotherapy. Blood 1997;89:1846-1853.

Qian J, Qin S, He Z, Wang L, Chen Y, Shao Z, et al. Clinical study of arsenic trioxide in the treatment of advanced primary liver cancer. Chin J Oncol (Chin) 2001;23:487-489.

Hose D, Réme T, Meissner T, Moreaux J, Seckinger A, Lewis J, et al. Inhibition of aurora kinases for tailored risk-adapted treatment of multiple myeloma. Blood 2009;113:4331-4340.

Chng WJ, Braggio E, Muligan G, Bryant B, Remstein E, Valdez R, et al. The centrosome index is a powerful prognostic marker in myeloma and identifies a cohort of patients that might benefit from aurora kinase inhibition. Blood 2008;111:1603-1609.

Stewart S, Fang G. Destruction box-dependent degradation of Aurora B is mediated by the anaphase-promoting complex/cyclosome and Cdh1. Cancer Res 2005;65:8730-8735.

Lee SH, Kim HS, Park WS, Kim SY, Lee KY, Kim SH, et al. Non-small cell lung cancers frequently express phosphorylated Akt; an immunohistochemical study. APMIS 2002;110:587-592.

Saito M, Korsmeyer SJ, Schlesinger PH. BAX-dependent transport of cytochrome reconstituted in pure liposomes. Nat Cell Biol 2000;2:553-555.

Pearse RN, Sordilo EM, Yaccoby S, Wonq BR, Liau DF, Colman N, et al. Multiple myeloma disrupts the TRANCE/osteoprotegerin cytoline axis to trigger bone destruction and promote tumor progression. Proc Nat Acad Sci USA 2001;98:11581-11586.

Han JH, Choi SJ, Kurihara N, Koide M, Oba Y, Roodman GD. Macrophage inflammatory protein-1 alpha is an osteoclastogenic factor in myeloma that is independent of receptor activator of nuclear kappa B ligand. Blood 2001;97:3349-3353.

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Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran

Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran

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Department of Nursing, Min-Hwei College of Health Care Management, Tainan

Division of Endocrinology and Metabolism, Kaohsiung Veteran General Hospital Tainan Branch, Tainan

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