Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury

Ming Jin; Chun-yan Sun; Bao-xia Zang

doi:10.1007/s11655-015-1976-x

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Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury

Updated：2021-08-27

- Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury
- Chinese Journal of Integrative Medicine Vol. 22, Issue 1, Pages: 36-41(2016)
- Affiliations：
  
  Department of Pharmacology, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University,Beijing,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81270103) and Natural Science Foundation of Beijing (No. 7102025)
- DOI：10.1007/s11655-015-1976-x
  CLC：
- Published：2016，
  
  Published Online：27 May 2015，
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Jin, M., Sun, Cy. & Zang, Bx. Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury., Chin. J. Integr. Med. 22, 36–41 (2016). https://doi.org/10.1007/s11655-015-1976-x

Ming Jin, Chun-yan Sun, Bao-xia Zang. Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury. [J]. Chinese Journal of Integrative Medicine 22(1):36-41(2016)
Jin, M., Sun, Cy. & Zang, Bx. Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury., Chin. J. Integr. Med. 22, 36–41 (2016). https://doi.org/10.1007/s11655-015-1976-x DOI：

Ming Jin, Chun-yan Sun, Bao-xia Zang. Hydroxysafflor yellow A attenuate lipopolysaccharide-induced endothelium inflammatory injury. [J]. Chinese Journal of Integrative Medicine 22(1):36-41(2016) DOI： 10.1007/s11655-015-1976-x.

摘要

This study observed attenuating effect of hydroxysafflor yellow A (HSYA)

an effective ingredient of aqueous extract of Carthamus tinctorius L

on lipopolysaccharide (LPS)-induced endothelium inflammatory injury. Eahy926 human endothelium cell (EC) line was used; thiazolyl blue tetrazolium bromide (MTT) test was assayed to observe the viability of EC; Luciferase reporter gene assay was applied to measure nuclear factor-κB (NF-κB) p65 subunit nuclear binding activity in EC; Western blot technology was used to monitor mitogen activated protein kinase (MAPKs) and NF-κB activation. Reverse transcription polymerase chain reaction (RT-PCR) method was applied to observe intercellular cell adhesion molecule-1 (ICAM-1) and E-selectin mRNA level; EC surface ICAM-1 expression was measured with flow cytometry and leukocyte adhesion to EC was assayed with Rose Bengal spectrophotometry technology. HSYA protected EC viability against LPS-induced injury (P <0.05). LPS-induced NF-κB p65 subunit DNA binding (P <0.01) and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IκBα) phosphorylation was inhibited by HSYA. HSYA attenuated LPS triggered ICAM-1 and E-selectin mRNA levels elevation and phosphorylation of p38 MAPK or c-Jun N-terminal kinase MAPK. HSYA also inhibited LPS-induced cell surface ICAM-1 protein expression P <0.01) and leukocyte adhesion to EC (P <0.05). HSYA is effective to protect LPS-induced high expression of endothelium adhesive molecule and inflammatory signal transduction.

Abstract

This study observed attenuating effect of hydroxysafflor yellow A (HSYA)

an effective ingredient of aqueous extract of Carthamus tinctorius L

关键词

hydroxysafflor yellow Alipopolysaccharideendothelium cellinflammationadhesive molecule

Keywords

hydroxysafflor yellow Alipopolysaccharideendothelium cellinflammationadhesive molecule

references

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Related Institution

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Ministry of Education), Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College

State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process

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Xiyuan Hospital, China Academy of Chinese Medical Sciences

Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine

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