Xuezhikang (血脂康) reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family

Wei-na Lu; Fen-ping Zheng; Dong-wu Lai; Hong Li

doi:10.1007/s11655-015-2050-4

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Xuezhikang (血脂康) reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family

Updated：2021-08-27

- Xuezhikang (血脂康) reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family
- Chinese Journal of Integrative Medicine Vol. 22, Issue 8, Pages: 611-618(2016)
- Affiliations：
  
  1. Department of Endocrinology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University,Hangzhou,China
  2. Department of Cardiovascular, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University,Hangzhou,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2050-4
  CLC：
- Published：2016，
  
  Published Online：6 April 2015，
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Lu, Wn., Zheng, Fp., Lai, Dw. et al. Xuezhikang (血脂康) reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family., Chin. J. Integr. Med. 22, 611–618 (2016). https://doi.org/10.1007/s11655-015-2050-4

Wei-na Lu, Fen-ping Zheng, Dong-wu Lai, et al. Xuezhikang (血脂康) reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family. [J]. Chinese Journal of Integrative Medicine 22(8):611-618(2016)
Lu, Wn., Zheng, Fp., Lai, Dw. et al. Xuezhikang (血脂康) reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family., Chin. J. Integr. Med. 22, 611–618 (2016). https://doi.org/10.1007/s11655-015-2050-4 DOI：

Wei-na Lu, Fen-ping Zheng, Dong-wu Lai, et al. Xuezhikang (血脂康) reduced renal cell apoptosis in streptozocin-induced diabetic rats through regulation of Bcl-2 family. [J]. Chinese Journal of Integrative Medicine 22(8):611-618(2016) DOI： 10.1007/s11655-015-2050-4.

摘要

To investigate the effect of Xuezhikang (血脂康

XZK) on renal cell apoptosis in diabetic rats and the possible mechanism. Sixty-six rats were randomly divided into 3 groups: the normal

model and XZK groups. In each group

the rats were further randomly divided into 3-month and 6-month subgroups

respectively. Diabetes of rats was induced by a single intraperitoneal injection of 1% streptozocin at 60 mg/kg body weight. Rats in the XZK group received gastric perfusion of XZK (1200 mg/kg body weight) everyday for 3 or 6 months

while rats in the normal and model groups received equal volume of saline. Twenty-four hours’ urine was collected for urinary albumin excretion (UAE) measurement. Periodic acid-Schiff (PAS) and Masson’s trichrome staining were used for saccharides and collagen detection. Cell apoptosis of renal cortex was investigated by TdT-mediated dUTP nick end labeling (TUNEL) staining. Bax and Bcl-2 expressions were detected by immunohistochemistry and Western blot

respectively. Cytochrome C (Cyt C) and caspase-9 concentration were detected by Western blot. Compared with the model group

XZK treatment could significantly decrease the kidney hypertrophy index

24 h UAE

renal cell apoptosis

cytoplasmic Cyt C level and active caspase-9 level

as well as suppress the increment of Bax and up-regulate the expression of Bcl-2

leading to the suppression of Bax/Bcl-2 ratio at 3 and 6 months (P<0.05 or P<0.01). Moreover

XZK treatment could alleviate the deposition of PAS-stained saccharides and Masson’s trichromestained collagen to different extent. Renal cell apoptosis was observed in diabetic kidney

in which mitochondrial apoptotic pathway might be involved. XZK treatment could attenuate pathological changes in diabetic kidney and reduce renal cell apoptosis

probably via the suppression of Bax/Bcl-2 ratio

which lead to inhibition of Cyt C release and following caspase-9 activation.

Abstract

To investigate the effect of Xuezhikang (血脂康

XZK) on renal cell apoptosis in diabetic rats and the possible mechanism. Sixty-six rats were randomly divided into 3 groups: the normal

model and XZK groups. In each group

the rats were further randomly divided into 3-month and 6-month subgroups

respectively. Cytochrome C (Cyt C) and caspase-9 concentration were detected by Western blot. Compared with the model group

XZK treatment could significantly decrease the kidney hypertrophy index

24 h UAE

renal cell apoptosis

cytoplasmic Cyt C level and active caspase-9 level

as well as suppress the increment of Bax and up-regulate the expression of Bcl-2

leading to the suppression of Bax/Bcl-2 ratio at 3 and 6 months (P<0.05 or P<0.01). Moreover

XZK treatment could alleviate the deposition of PAS-stained saccharides and Masson’s trichromestained collagen to different extent. Renal cell apoptosis was observed in diabetic kidney

in which mitochondrial apoptotic pathway might be involved. XZK treatment could attenuate pathological changes in diabetic kidney and reduce renal cell apoptosis

probably via the suppression of Bax/Bcl-2 ratio

which lead to inhibition of Cyt C release and following caspase-9 activation.

关键词

XuezhikangdiabetesapoptosisBcl-2 familymitochondrial pathwayChinese Medicine

Keywords

XuezhikangdiabetesapoptosisBcl-2 familymitochondrial pathwayChinese Medicine

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Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine

Shanghai Key Laboratory of Complex Prescription

Chinese Medicine Hospital of Shanxi Province

Department of Gastroenterology, the Second Affiliated Hospital, Xi’an Jiaotong University

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