Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice

Ying-wan Liu; Wei-feng Sun; Xian-xian Zhang; Jing Li; Huan-huan Zhang

doi:10.1007/s11655-015-2052-2

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Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice

Updated：2021-08-27

- Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice
- Chinese Journal of Integrative Medicine Vol. 21, Issue 11, Pages: 823-829(2015)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, General Hospital of Guangzhou Military Command of PLA,Guangzhou,China
  2. Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2052-2
  CLC：
- Published：2015，
  
  Published Online：11 April 2015，
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Liu, Yw., Sun, Wf., Zhang, Xx. et al. Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice., Chin. J. Integr. Med. 21, 823–829 (2015). https://doi.org/10.1007/s11655-015-2052-2

Ying-wan Liu, Wei-feng Sun, Xian-xian Zhang, et al. Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice. [J]. Chinese Journal of Integrative Medicine 21(11):823-829(2015)
Liu, Yw., Sun, Wf., Zhang, Xx. et al. Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice., Chin. J. Integr. Med. 21, 823–829 (2015). https://doi.org/10.1007/s11655-015-2052-2 DOI：

Ying-wan Liu, Wei-feng Sun, Xian-xian Zhang, et al. Compound Tufuling Granules (复方土茯苓颗粒) regulate glucose transporter 9 expression in kidney to influence serum uric acid level in hyperuricemia mice. [J]. Chinese Journal of Integrative Medicine 21(11):823-829(2015) DOI： 10.1007/s11655-015-2052-2.

摘要

To explore the effect of Compound Tufuling Granules (复方土茯苓颗粒

CTG) on regulating glucose transporter 9 (GLUT9) expression in the kidney to influence the uric acid excretion by the kidney and serum uric acid (SUA) level in hyperuricemia mice. Sixty Kunming male mice were randomly divided into the control group

model group

benzbromarone group

and CTG high-

middle- and lowdose groups. The yeast extract and uricase inhibition method were used to build hyperuricemia model

and the corresponding drugs were administrated on the 7th day. On the 21st day the 24-h urine was collected

on the 22nd day the blood was collected

the SUA level was detected by uricase colorimetry

and the mRNA and protein expressions of GLUT9 were detected by quantitative real-time polymerase chain reaction and Western blot

respectively. Compared with the model group

the levels of SUA and the mRNA and protein expressions of GLUT9 were signifificantly decreased

and the fraction excretion of uric acid (FEUA) was significantly increased in the CTG groups and benzbromarone group (all P<0.05). There was no significant difference in the above indicators between the CTG high-dose group and benzbromarone group (P>0.05). SUA is positively related to the GLUT9 mRNA and protein expressions in the kidney (P<0.05 or P<0.01). CTG can signifificantly reduce the SUA and increase the FEUA. In addition

CTG can effectively inhibit the mRNA and protein expressions of GLUT9 in the kidney of hyperuricemia mice to inhibit the uric acid re-absorption

promote uric acid excretion and reduce SUA.

Abstract

To explore the effect of Compound Tufuling Granules (复方土茯苓颗粒

model group

benzbromarone group

and CTG high-

middle- and lowdose groups. The yeast extract and uricase inhibition method were used to build hyperuricemia model

and the corresponding drugs were administrated on the 7th day. On the 21st day the 24-h urine was collected

on the 22nd day the blood was collected

the SUA level was detected by uricase colorimetry

and the mRNA and protein expressions of GLUT9 were detected by quantitative real-time polymerase chain reaction and Western blot

respectively. Compared with the model group

the levels of SUA and the mRNA and protein expressions of GLUT9 were signifificantly decreased

CTG can effectively inhibit the mRNA and protein expressions of GLUT9 in the kidney of hyperuricemia mice to inhibit the uric acid re-absorption

promote uric acid excretion and reduce SUA.

关键词

Compound Tufuling Granuleshyperuricemiaglucose transporter 9uric acidChinese Medicine

Keywords

Compound Tufuling Granuleshyperuricemiaglucose transporter 9uric acidChinese Medicine

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