Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome

Ming-ming Wang; Mei-xia Fang; Li-guo Chen; Hua-qiang Wang; Hong-jie Liu; Hai-lan Tang

doi:10.1007/s11655-015-2053-1

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Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome

Updated：2021-08-27

- Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome
- Chinese Journal of Integrative Medicine Vol. 21, Issue 11, Pages: 817-822(2015)
- Affiliations：
  
  Faculty of Traditional Chinese Medicine, Medical School of Jinan University,Guangzhou,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2053-1
  CLC：
- Published：2015，
  
  Published Online：11 April 2015，
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Wang, Mm., Fang, Mx., Chen, Lg. et al. Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome., Chin. J. Integr. Med. 21, 817–822 (2015). https://doi.org/10.1007/s11655-015-2053-1

Ming-ming Wang, Mei-xia Fang, Li-guo Chen, et al. Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome. [J]. Chinese Journal of Integrative Medicine 21(11):817-822(2015)
Wang, Mm., Fang, Mx., Chen, Lg. et al. Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome., Chin. J. Integr. Med. 21, 817–822 (2015). https://doi.org/10.1007/s11655-015-2053-1 DOI：

Ming-ming Wang, Mei-xia Fang, Li-guo Chen, et al. Differential expression of microRNA in endothelial cells incubated with serum of hypertension patients with blood-stasis syndrome. [J]. Chinese Journal of Integrative Medicine 21(11):817-822(2015) DOI： 10.1007/s11655-015-2053-1.

摘要

To screen out blood-stasis syndrome (BSS)-associated microRNA and therefore determine the possible target for treating hypertension. A high-energy sequencing method and digital gene expression sequencing theory were adopted to sequence microRNA (miRNA) and messenger RNA (mRNA)

and to determine differential expression in human umbilical vein endothelial cells incubated with serum samples from hypertension patients with or without BSS

and healthy controls. The results were confifirmed using gene prediction software. A total of 13 miRNAs and 11 mRNAs showed statistical difference both in the BSS/normal groups and BSS/non-BSS groups

respectively. Four pairs of target mRNA/miRNA were identifified: FRMD4A/hsa-miR-34a

MAP3K14/hsa-miR-34a

PER1/hsa-miR-34a

and FGF2/hsa-miR-132. Four mRNA/miRNA pairs mentioned above seem to be involved in pathogenesis and maintenance of hypertension with BSS.

Abstract

and to determine differential expression in human umbilical vein endothelial cells incubated with serum samples from hypertension patients with or without BSS

and healthy controls. The results were confifirmed using gene prediction software. A total of 13 miRNAs and 11 mRNAs showed statistical difference both in the BSS/normal groups and BSS/non-BSS groups

respectively. Four pairs of target mRNA/miRNA were identifified: FRMD4A/hsa-miR-34a

MAP3K14/hsa-miR-34a

PER1/hsa-miR-34a

and FGF2/hsa-miR-132. Four mRNA/miRNA pairs mentioned above seem to be involved in pathogenesis and maintenance of hypertension with BSS.

关键词

hypertensionblood-stasis syndromemicroRNAmessenger RNAChinese Medicine

Keywords

hypertensionblood-stasis syndromemicroRNAmessenger RNAChinese Medicine

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Department of Cardiovascular Disease, Xiyuan Hospital, China Academy of Chinese Medical Sciences

Xiyuan Hospital, China Academy of Chinese Medical Sciences

Traditional Chinese Medicine Hospital of Yanqing County

Community Health Service Centers of Yanqing County

Department of Dermatology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Affiliated to Shanghai University of Traditional Chinese Medicine

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