Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats

Yi-ling Liu; Xian-lin Zhao; Juan Li; Mei-hua Wan; Guang-yuan Chen; Wei-wei Chen; Wen-fu Tang

doi:10.1007/s11655-015-2080-y

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Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats

Updated：2021-08-27

- Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats
- Chinese Journal of Integrative Medicine Vol. 21, Issue 12, Pages: 922-927(2015)
- Affiliations：
  
  Department of Integrative Medicine, West China Hospital, Sichuan University,Chengdu,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-015-2080-y
  CLC：
- Published：2015，
  
  Published Online：2 July 2015，
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Liu, Yl., Zhao, Xl., Li, J. et al. Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats., Chin. J. Integr. Med. 21, 922–927 (2015). https://doi.org/10.1007/s11655-015-2080-y

Yi-ling Liu, Xian-lin Zhao, Juan Li, et al. Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats. [J]. Chinese Journal of Integrative Medicine 21(12):922-927(2015)
Liu, Yl., Zhao, Xl., Li, J. et al. Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats., Chin. J. Integr. Med. 21, 922–927 (2015). https://doi.org/10.1007/s11655-015-2080-y DOI：

Yi-ling Liu, Xian-lin Zhao, Juan Li, et al. Effect of acute pancreatitis on the pharmacokinetics of Chinese herbal micron Liuhe Pill ointment (微米六合丹) in rats. [J]. Chinese Journal of Integrative Medicine 21(12):922-927(2015) DOI： 10.1007/s11655-015-2080-y.

摘要

To explore the effect of acute pancreatitis (AP) on the pharmacokinetics of herbal ointment micron Liuhe Pill (微米六合丹

MLHP) components in anesthetized rats. Rats were randomly divided into a AP model group (n=6) and a normal group as a control (n=6). The rat model of AP was induced by intraperitoneal injection of L-arginine in rats (15 mg/kg

twice

interval 1 h). Chinese herbal ointment MLHP was used externally on the belly after the 2nd injection for 48 h in both groups. Emodin

rhein

aloe emodin

physcion

chrysophanol from MLHP were detected and quantified in rat serum and pancreas (at 48 h) by high performance liquid chromatography-tandem mass spectrometry. Among the five components

only emodin

aloe emodin and physcion from MLHP were detected in all rat serum and most of the rats' pancreas. Rhein and chrysophanol were not detected in both serum and pancreas. T1/2α of emodin and physcion in MLHP were obviously shorter in the AP model group than those in the normal group (P<0.05)

while there was no difference for T1/2α of aloe emodin. The peak concentration and area under curve of all three components were much higher in the AP group than those in the normal group with MLHP in external application for 48 h (P<0.05). Furthermore

the mean residence time (MRT) and maximum plasma concentration (Tmax) of emodin and aloe emodin were obviously longer in the AP model group than those in the normal control group (P<0.05). There was no significant difference for Ka of all components between the two groups. Emodin could be detected in all rats' pancreas at 48 h in both groups

while its mean pancreatic concentration was higher in the AP model group than in the normal group (0.61±0.54 ng/mL

0.42±0.37 ng/mL

respectively

P<0.05). Aloe emodin could be detected in all rats' pancreas at 48 h in both groups and their mean pancreatic concentration were similar (0.31±0.24 ng/mL

0.33±0.17 ng/mL

respectively

P>0.05). Physcion could be detected in pancreas of most rats in the AP model while only two rats in the normal group. AP could significantly affect the pharmacokinetics of absorbed components of Chinese herbal MLHP ointment in rats.

Abstract

To explore the effect of acute pancreatitis (AP) on the pharmacokinetics of herbal ointment micron Liuhe Pill (微米六合丹

twice

interval 1 h). Chinese herbal ointment MLHP was used externally on the belly after the 2nd injection for 48 h in both groups. Emodin

rhein

aloe emodin

physcion

chrysophanol from MLHP were detected and quantified in rat serum and pancreas (at 48 h) by high performance liquid chromatography-tandem mass spectrometry. Among the five components

only emodin

while its mean pancreatic concentration was higher in the AP model group than in the normal group (0.61±0.54 ng/mL

0.42±0.37 ng/mL

respectively

P<0.05). Aloe emodin could be detected in all rats' pancreas at 48 h in both groups and their mean pancreatic concentration were similar (0.31±0.24 ng/mL

0.33±0.17 ng/mL

respectively

关键词

Chinese herbal ointmentmicron Liuhe PillpharmacokineticsAcute Pancreatitis

Keywords

Chinese herbal ointmentmicron Liuhe PillpharmacokineticsAcute Pancreatitis

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