Sodium tanshinone II A sulfonate ameliorates microcirculatory disturbance of small intestine by attenuating the production of reactie oxygen species in rats with sepsis

Wei Zhu; Qing Lu; Lei Wan; Jun Feng; Hua-wen Chen

doi:10.1007/s11655-015-2083-8

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Sodium tanshinone II A sulfonate ameliorates microcirculatory disturbance of small intestine by attenuating the production of reactie oxygen species in rats with sepsis

Updated：2021-08-27

- Sodium tanshinone II A sulfonate ameliorates microcirculatory disturbance of small intestine by attenuating the production of reactie oxygen species in rats with sepsis
- Chinese Journal of Integrative Medicine Vol. 22, Issue 10, Pages: 745-751(2016)
- Affiliations：
  
  1. Department of Emergency Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
  2. Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30500657) and Natural Science Foundation of Hubei Provience (No. 2009CDB371)
- DOI：10.1007/s11655-015-2083-8
  CLC：
- Published：2016，
  
  Published Online：26 August 2015，
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Zhu, W., Lu, Q., Wan, L. et al. Sodium tanshinone II A sulfonate ameliorates microcirculatory disturbance of small intestine by attenuating the production of reactie oxygen species in rats with sepsis., Chin. J. Integr. Med. 22, 745–751 (2016). https://doi.org/10.1007/s11655-015-2083-8

Wei Zhu, Qing Lu, Lei Wan, et al. Sodium tanshinone II A sulfonate ameliorates microcirculatory disturbance of small intestine by attenuating the production of reactie oxygen species in rats with sepsis. [J]. Chinese Journal of Integrative Medicine 22(10):745-751(2016)
Zhu, W., Lu, Q., Wan, L. et al. Sodium tanshinone II A sulfonate ameliorates microcirculatory disturbance of small intestine by attenuating the production of reactie oxygen species in rats with sepsis., Chin. J. Integr. Med. 22, 745–751 (2016). https://doi.org/10.1007/s11655-015-2083-8 DOI：

Wei Zhu, Qing Lu, Lei Wan, et al. Sodium tanshinone II A sulfonate ameliorates microcirculatory disturbance of small intestine by attenuating the production of reactie oxygen species in rats with sepsis. [J]. Chinese Journal of Integrative Medicine 22(10):745-751(2016) DOI： 10.1007/s11655-015-2083-8.

摘要

To examine whether sodium tanshinone II A sulfonate (STS)

the main effective component of Salvia miltiorrhiza is effective in relieving the microcirculatory disturbance of small intestine by suppressing the production of reactive oxygen species (ROS) in rats with sepsis. A rat model of sepsis was induced by cecal ligation and puncture (CLP). Rats (n =40) were randomly divided into 4 groups: sham-operated group (sham

n =10)

sepsis group (CLP

n =10)

STS treatment group (STS

n =10) and ROS scavenger dimethylthiourea (DMTU

n =10) group. Animals in the STS group were injected with STS (1 mg/kg) for 10 min through the right external jugular vein after the CLP operation

and animals in the CLP group were given the same volume of normal saline after the CLP operation. Animals in the DMTU group were intraperitoneally injected with 5 mL/kg of 20% DMTU 1 h before CLP. The histopathologic changes in the intestinal tissues and changes of mesenteric microcirculation were observed. The levels of ROS in intestinal tissues from each group were qualitatively evaluated using a fluorescent microscope. The expressions of apoptosis signal-regulating kinase (ASK1)

phosphorylated ASK1 (phospho-ASK1)

p38 mitogen-activated protein kinases (p38 MAPK)

phosphorylated p38 MAPK (phospho-p38 MAPK) and tissue factor (TF) were determined by Western blotting. It was shown that there were obvious microcirculatory disturbance (P <0.05) and tissue injuries in intestinal tissues after CLP operation. The levels of ROS production

phospho-ASK1

phospho-p38 MAPK and TF were increased. Both STS and DMTU suppressed ROS

phospho-ASK1

phospho-p38 MAPK and TF production

and ameliorated the microcirculatory disturbance and tissues injury (P <0.01). STS can ameliorate the microcirculatory disturbance of the small intestine by attenuating the production of ROS in rats with sepsis.

Abstract

To examine whether sodium tanshinone II A sulfonate (STS)

n =10)

sepsis group (CLP

n =10)

STS treatment group (STS

n =10) and ROS scavenger dimethylthiourea (DMTU

n =10) group. Animals in the STS group were injected with STS (1 mg/kg) for 10 min through the right external jugular vein after the CLP operation

phosphorylated ASK1 (phospho-ASK1)

p38 mitogen-activated protein kinases (p38 MAPK)

phospho-ASK1

phospho-p38 MAPK and TF were increased. Both STS and DMTU suppressed ROS

phospho-ASK1

phospho-p38 MAPK and TF production

关键词

tanshinone II Asepsismicrocirculationreactive oxygen speciesChinese Medicine

Keywords

tanshinone II Asepsismicrocirculationreactive oxygen speciesChinese Medicine

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