Effects of Senegenin against hypoxia/reoxygenation-induced injury in PC12 cells

Xiao-qing Zhu; Xue-min Li; Yan-dong Zhao; Xi-luan Ji; Yan-ping Wang; Yong-mei Fu; Hua-dong Wang; Da-xiang Lu; Ren-bin Qi

doi:10.1007/s11655-015-2091-8

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Effects of Senegenin against hypoxia/reoxygenation-induced injury in PC12 cells

Updated：2021-08-27

- Effects of Senegenin against hypoxia/reoxygenation-induced injury in PC12 cells
- Chinese Journal of Integrative Medicine Vol. 22, Issue 5, Pages: 353-361(2016)
- Affiliations：
  
  1. Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine of the People’s Republic of China, School of Medicine, Jinan University,Guangzhou,China
  2. Clifford Hospital, Guangzhou University of Chinese Medicine,Guangzhou,China
  3. High Magnetic Field Laboratory, Chinese Academy of Sciences,Hefei,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of Guangdong Province, China (No. 2014A030313394), the Project of Science and Technology of Guangzhou, China (No. 2014J4100098), the National Program on Key Basic Research Project (973 Program, No. 2011CB707501), and the Fundamental Research Funds for the Central Universities in China (No. 21613401)
- DOI：10.1007/s11655-015-2091-8
  CLC：
- Published：2016，
  
  Published Online：12 January 2016，
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Zhu, Xq., Li, Xm., Zhao, Yd. et al. Effects of Senegenin against hypoxia/reoxygenation-induced injury in PC12 cells., Chin. J. Integr. Med. 22, 353–361 (2016). https://doi.org/10.1007/s11655-015-2091-8

Xiao-qing Zhu, Xue-min Li, Yan-dong Zhao, et al. Effects of Senegenin against hypoxia/reoxygenation-induced injury in PC12 cells. [J]. Chinese Journal of Integrative Medicine 22(5):353-361(2016)
Zhu, Xq., Li, Xm., Zhao, Yd. et al. Effects of Senegenin against hypoxia/reoxygenation-induced injury in PC12 cells., Chin. J. Integr. Med. 22, 353–361 (2016). https://doi.org/10.1007/s11655-015-2091-8 DOI：

Xiao-qing Zhu, Xue-min Li, Yan-dong Zhao, et al. Effects of Senegenin against hypoxia/reoxygenation-induced injury in PC12 cells. [J]. Chinese Journal of Integrative Medicine 22(5):353-361(2016) DOI： 10.1007/s11655-015-2091-8.

摘要

To investigate the effect and the potential mechanism of Senegenin (Sen) against injury induced by hypoxia/reoxygenation (H/R) in highly differentiated PC12 cells. The cultured PC12 cells were treated with H/R in the presence or absence of Sen (60 μmol/L). Four groups were included in the experiment: control group

H/R group

H/R+Sen group and Sen group. Cell viability of each group and the level of lactate dehydrogenase (LDH) in culture medium were detected for the pharmacological effect of Sen. Hoechst 33258 staining and annexin V/propidium iodide double staining were used to analyze the apoptosis rate. Moreover

mitochondrial membrane potential (△Ψm)

reactive oxygen species (ROS) and intracellular free calcium ([Ca2+]i) were measured by fluorescent staining and flow cytometry. Cleaved caspase-3 and activity of NADPH oxidase (NOX) were determined by colorimetric protease assay and enzyme linked immunosorbent assay

respectively. Sen significantly elevated cell viability (P<0.05)

decreased the leakage of LDH (P<0.05) and apoptosis rate (P<0.05) in H/R-injured PC12 cells. Sen maintained the value of △Ψm (P<0.05) and suppressed the activity of caspase-3 (P<0.05). Moreover

Sen reduced ROS accumulation P<0.05) and [Ca2+]i increment (P<0.05) by inhibiting the activity of NOX (P<0.05). Sen may exert cytoprotection against H/R injury by decreasing the levels of intracellular ROS and [Ca2+]i

thereby suppressing the mitochondrial pathway of cellular apoptosis.

Abstract

H/R group

mitochondrial membrane potential (△Ψm)

respectively. Sen significantly elevated cell viability (P<0.05)

decreased the leakage of LDH (P<0.05) and apoptosis rate (P<0.05) in H/R-injured PC12 cells. Sen maintained the value of △Ψm (P<0.05) and suppressed the activity of caspase-3 (P<0.05). Moreover

thereby suppressing the mitochondrial pathway of cellular apoptosis.

关键词

senegeninhypoxia/reoxygenationPC12 cellsreactive oxygen speciesNAPDH oxidase

Keywords

senegeninhypoxia/reoxygenationPC12 cellsreactive oxygen speciesNAPDH oxidase

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