Anti-oxidative and anti-inflammatory effects of cinnamaldehyde on protecting high glucose-induced damage in cultured dorsal root ganglion neurons of rats

Dan Yang; Xiao-chun Liang; Yue Shi; Qing Sun; Di Liu; Wei Liu; Hong Zhang

doi:10.1007/s11655-015-2103-8

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Anti-oxidative and anti-inflammatory effects of cinnamaldehyde on protecting high glucose-induced damage in cultured dorsal root ganglion neurons of rats

Updated：2021-08-27

- Anti-oxidative and anti-inflammatory effects of cinnamaldehyde on protecting high glucose-induced damage in cultured dorsal root ganglion neurons of rats
- Chinese Journal of Integrative Medicine Vol. 22, Issue 1, Pages: 19-27(2016)
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Translational Medicine Center, Chinese Academy of Medical Sciences,Beijing,China
  2. Department of Cell Resource Center, Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81473639) and the Natural Science Foundation of Beijing (No. 7122147)
- DOI：10.1007/s11655-015-2103-8
  CLC：
- Published：2016，
  
  Published Online：17 November 2015，
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Yang, D., Liang, Xc., Shi, Y. et al. Anti-oxidative and anti-inflammatory effects of cinnamaldehyde on protecting high glucose-induced damage in cultured dorsal root ganglion neurons of rats., Chin. J. Integr. Med. 22, 19–27 (2016). https://doi.org/10.1007/s11655-015-2103-8

Dan Yang, Xiao-chun Liang, Yue Shi, et al. Anti-oxidative and anti-inflammatory effects of cinnamaldehyde on protecting high glucose-induced damage in cultured dorsal root ganglion neurons of rats. [J]. Chinese Journal of Integrative Medicine 22(1):19-27(2016)
Yang, D., Liang, Xc., Shi, Y. et al. Anti-oxidative and anti-inflammatory effects of cinnamaldehyde on protecting high glucose-induced damage in cultured dorsal root ganglion neurons of rats., Chin. J. Integr. Med. 22, 19–27 (2016). https://doi.org/10.1007/s11655-015-2103-8 DOI：

Dan Yang, Xiao-chun Liang, Yue Shi, et al. Anti-oxidative and anti-inflammatory effects of cinnamaldehyde on protecting high glucose-induced damage in cultured dorsal root ganglion neurons of rats. [J]. Chinese Journal of Integrative Medicine 22(1):19-27(2016) DOI： 10.1007/s11655-015-2103-8.

摘要

To examine the mechanism underlying the beneficial role of cinnamaldehyde on oxidative damage and apoptosis in high glucose (HG)-induced dorsal root ganglion (DRG) neurons in vitro. HG-treated DRG neurons were developed as an in vitro model of diabetic neuropathy. The neurons were randomly divided into five groups: the control group

the HG group and the HG groups treated with 25

50 and 100 nmol/L cinnamaldehyde

respectively. Cell viability was examined by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay and apoptosis rate was evaluated by the in situ TdT-mediated dUTP nick end labeling (TUNEL) assay. The intracellular level of reactive oxygen species (ROS) was measured with flow cytometry. Expression of nuclear factor-kappa B (NF-κB)

inhibitor of κB (IκB)

phosphorylated IκB (p-IκB)

tumor necrosis factor (TNF)-α

interleukin-6 (IL-6) and caspase-3 were determined by western blotting and real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1) were also measured by western blotting. Cinnamaldehyde reduced HG-induced loss of viability

apoptosis and intracellular generation of ROS in the DRG neurons via inhibiting NF-κB activity. The western blot assay results showed that the HG-induced elevated expressions of NF-κB

IκB and p-IκB were remarkably reduced by cinnamaldehyde treatment in a dose-dependent manner (P <0.01). The HG-induced over-expression of NF-κB p65 mRNA was remarkably attenuated after cinnamaldehyde treatment in a dose-dependent manner (P <0.01). However

the expressions of Nrf2 and HO-1 were not upregulated. Treatment with cinnamaldehyde not only attenuated caspase-3 activation and the caspase cleavage cascade in DRG neurons

but also lowered the elevated IL-6

TNF-α

cyclo-oxygenase and inducible nitric oxide synthase levels

indicating a reduction in inflammatory damage. Cinnamaldehyde protected DRG neurons from the deleterious effects of HG through inactivation of NF-κB pathway but not through activation of Nrf2/HO-1. And thus cinnamaldehyde may have potential application as a treatment for DPN.

Abstract

the HG group and the HG groups treated with 25

50 and 100 nmol/L cinnamaldehyde

respectively. Cell viability was examined by 3-(4

5-dimethylthiazol-2-yl)-2

inhibitor of κB (IκB)

phosphorylated IκB (p-IκB)

tumor necrosis factor (TNF)-α

apoptosis and intracellular generation of ROS in the DRG neurons via inhibiting NF-κB activity. The western blot assay results showed that the HG-induced elevated expressions of NF-κB

the expressions of Nrf2 and HO-1 were not upregulated. Treatment with cinnamaldehyde not only attenuated caspase-3 activation and the caspase cleavage cascade in DRG neurons

but also lowered the elevated IL-6

TNF-α

cyclo-oxygenase and inducible nitric oxide synthase levels

关键词

nuclear factor-kappa Bnuclear factor erythroid 2-related factor 2cinnamaldehydehigh glucosedorsal root ganglion

Keywords

nuclear factor-kappa Bnuclear factor erythroid 2-related factor 2cinnamaldehydehigh glucosedorsal root ganglion

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Pharmacy College, Jinan University

State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences

Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences

Cell Center, Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences

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