FOLLOWUS
Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation
- Chinese Journal of Integrative Medicine Vol. 22, Issue 6, Pages: 430-437(2016)
- Affiliations：
1. Integrated Laboratory of Chinese and Western Medicine, Peking University First Hospital,Beijing,China
2. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences,Beijing,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 81102683 and No. 81173369), the Doctoral Fellowships of Ministry of Education of China (No. 20120001110105 and No. 20110001120055) and Beijing Natural Science Foundation (No. 7132210 )
DOI：10.1007/s11655-015-2116-3
CLC：Published：2016，
Published Online：20 November 2015，
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Song, Yx., Miao, Jy., Qiang, M. et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation., Chin. J. Integr. Med. 22, 430–437 (2016). https://doi.org/10.1007/s11655-015-2116-3
Yi-xiang Song, Jun-ye Miao, Min Qiang, et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation. [J]. Chinese Journal of Integrative Medicine 22(6):430-437(2016)
Song, Yx., Miao, Jy., Qiang, M. et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation., Chin. J. Integr. Med. 22, 430–437 (2016). https://doi.org/10.1007/s11655-015-2116-3 DOI：
Yi-xiang Song, Jun-ye Miao, Min Qiang, et al. Icariin protects SH-SY5Y cells from formaldehyde-induced injury through suppression of Tau phosphorylation. [J]. Chinese Journal of Integrative Medicine 22(6):430-437(2016) DOI： 10.1007/s11655-015-2116-3.
摘要
To investigate the neuroprotective effects of icariin on formaldehyde (FA)-treated human neuroblastoma SH-SY5Y cells and the possible mechanisms involved. SH-SY5Y cells were divided into FA treatment group
FA treatment group with icariin
and the control group. Cell viability
apoptosis
and morphological changes were determined by cell counting kit-8 (CCK 8)
flow cytometry
and confocal microscopy
respectively. The phosphorylation of Tau protein was examined by western blotting. FA showed a half lethal dose (LD50) of 0.3 mmol/L in SH-SY5Y cells under the experimental conditions. Icariin (1–10 µmol/L) prevented FA-induced cell death in SH-SY5Y cells in a dose-dependent manner
with the optimal effect observed at 5 µmol/L. After FA treatment
the absorbance in FA group was 1.31±0.05
while in the group of icariin (5 µmol/L) was 1.63±0.05. Examination of cell morphology by confocal microscopy demonstrated that 5 µmol/L icariin significantly attenuated FA-induced cell injury (P <0.05). Additionally
Icariin inhibited FA-induced cell apoptosis in SH-SY5Y cells. Results from western blotting showed that icariin suppressed FA-induced phosphorylation at Thr 181 and Ser 396 of Tau protein
while having no effect on the expression of the total Tau protein level. Furthermore
FA activated Tau kinase glycogen synthase kinase 3 beta (GSK-3β) by enhancement of Y216 phosphorylation
but icariin reduced Y216 phosphorylation and increased Ser 9 phosphorylation. Icariin protects SH-SY5Y cells from FA-induced injury poßsibly through the inhibition of GSK-3β-mediated Tau phosphorylation.
Abstract
To investigate the neuroprotective effects of icariin on formaldehyde (FA)-treated human neuroblastoma SH-SY5Y cells and the possible mechanisms involved. SH-SY5Y cells were divided into FA treatment group
FA treatment group with icariin
and the control group. Cell viability
apoptosis
and morphological changes were determined by cell counting kit-8 (CCK 8)
flow cytometry
and confocal microscopy
respectively. The phosphorylation of Tau protein was examined by western blotting. FA showed a half lethal dose (LD50) of 0.3 mmol/L in SH-SY5Y cells under the experimental conditions. Icariin (1–10 µmol/L) prevented FA-induced cell death in SH-SY5Y cells in a dose-dependent manner
with the optimal effect observed at 5 µmol/L. After FA treatment
the absorbance in FA group was 1.31±0.05
while in the group of icariin (5 µmol/L) was 1.63±0.05. Examination of cell morphology by confocal microscopy demonstrated that 5 µmol/L icariin significantly attenuated FA-induced cell injury (P <0.05). Additionally
Icariin inhibited FA-induced cell apoptosis in SH-SY5Y cells. Results from western blotting showed that icariin suppressed FA-induced phosphorylation at Thr 181 and Ser 396 of Tau protein
while having no effect on the expression of the total Tau protein level. Furthermore
FA activated Tau kinase glycogen synthase kinase 3 beta (GSK-3β) by enhancement of Y216 phosphorylation
but icariin reduced Y216 phosphorylation and increased Ser 9 phosphorylation. Icariin protects SH-SY5Y cells from FA-induced injury poßsibly through the inhibition of GSK-3β-mediated Tau phosphorylation.
关键词
icariinformaldehydeTau phosphorylationglycogen synthase kinase 3 betaneuroprotection
Keywords
icariinformaldehydeTau phosphorylationglycogen synthase kinase 3 betaneuroprotection
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