Shugan Liangxue Decoction (舒肝凉血方) Down-Regulates Estrogen Receptor α Expression in Breast Cancer Cells

Ning Zhou; Shu-yan Han; Yan-zhi Chen; Fei Zhou; Wen-xian Zheng; Ping-ping Li

doi:10.1007/s11655-015-2123-4

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Shugan Liangxue Decoction (舒肝凉血方) Down-Regulates Estrogen Receptor α Expression in Breast Cancer Cells

Updated：2021-08-27

- Shugan Liangxue Decoction (舒肝凉血方) Down-Regulates Estrogen Receptor α Expression in Breast Cancer Cells
- Chinese Journal of Integrative Medicine Vol. 24, Issue 7, Pages: 518-524(2018)
- Affiliations：
  
  Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Integration of Chinese and Western Medicine, Peking University Cancer Hospital Institute,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30973906);Now the author is working in the Department of Oncology, Beijing Ditan Hospital, Capital Medical University, Beijing (100015), China
- DOI：10.1007/s11655-015-2123-4
  CLC：
- Published：2018，
  
  Published Online：6 January 2016，
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Zhou, N., Han, Sy., Chen, Yz. et al. Shugan Liangxue Decoction (舒肝凉血方) Down-Regulates Estrogen Receptor α Expression in Breast Cancer Cells., Chin. J. Integr. Med. 24, 518–524 (2018). https://doi.org/10.1007/s11655-015-2123-4

Ning Zhou, Shu-yan Han, Yan-zhi Chen, et al. Shugan Liangxue Decoction (舒肝凉血方) Down-Regulates Estrogen Receptor α Expression in Breast Cancer Cells. [J]. Chinese Journal of Integrative Medicine 24(7):518-524(2018)
Zhou, N., Han, Sy., Chen, Yz. et al. Shugan Liangxue Decoction (舒肝凉血方) Down-Regulates Estrogen Receptor α Expression in Breast Cancer Cells., Chin. J. Integr. Med. 24, 518–524 (2018). https://doi.org/10.1007/s11655-015-2123-4 DOI：

Ning Zhou, Shu-yan Han, Yan-zhi Chen, et al. Shugan Liangxue Decoction (舒肝凉血方) Down-Regulates Estrogen Receptor α Expression in Breast Cancer Cells. [J]. Chinese Journal of Integrative Medicine 24(7):518-524(2018) DOI： 10.1007/s11655-015-2123-4.

摘要

To observe the effect of Shugan Liangxue Decoction (舒肝凉血方

SGLXD) on estrogen receptor α (ERα) in human breast cancer cells. The effect of SGLXD (0.85–5.10 mg/mL) on the proliferation of breast cancer cells were evaluated by 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The nuclear ERα protein levels in MCF-7

T47D and ZR-75-1 cells which treated by SGLXD for 24 h were examined by western blot and immunofluorescence assay. MCF-7 and MDA-MB-231 cells were treated by 17β-estradiol (E2) with or without SGLXD

for 24 h

and the E2 targeted genes c-myc and bcl-2 protein product was evaluated by western blot. SGLXD showed dose-dependent inhibition on the proliferation of MCF-7

T47D and ZR-75-1 cells

but did not inhibit the proliferation of MDA-MB-231 cells. Furthermore

the promotive effect on cell growth induced by E2 was also significantly inhibited by SGLXD treatment. With the treatment of 1.70

3.40

5.10 mg/mL SGLXD

the nuclear ERα protein level was reduced to 88.1%

70.4% and 60.9% in MCF-7 cells

and was decreased to 43.0%

38.4% and 5.9% in ZR-75-1 cells as compared with the control group. In T47D cells

the nuclear ERα protein was down-regulated to 51.3% and 4.3% by 3.40 and 5.10 mg/mL SGLXD treatment. The down-regulative effect of SGLXD on nuclear ERα was confirmed by immunofluorescence assay. SGLXD decreased the protein product of c-myc and bcl-2. SGLXD may exhibit selective inhibition effect on the proliferation of ER positive breast cancer cells. SGLXD reduced the nuclear ERα expression and the protein product of E2 target gene c-myc and bcl-2.

Abstract

To observe the effect of Shugan Liangxue Decoction (舒肝凉血方

SGLXD) on estrogen receptor α (ERα) in human breast cancer cells. The effect of SGLXD (0.85–5.10 mg/mL) on the proliferation of breast cancer cells were evaluated by 3-(4

5-dimethyl-2-thiazolyl)-2

5-diphenyl-2-H-tetrazolium bromide (MTT) assay. The nuclear ERα protein levels in MCF-7

T47D and ZR-75-1 cells which treated by SGLXD for 24 h were examined by western blot and immunofluorescence assay. MCF-7 and MDA-MB-231 cells were treated by 17β-estradiol (E2) with or without SGLXD

for 24 h

and the E2 targeted genes c-myc and bcl-2 protein product was evaluated by western blot. SGLXD showed dose-dependent inhibition on the proliferation of MCF-7

T47D and ZR-75-1 cells

but did not inhibit the proliferation of MDA-MB-231 cells. Furthermore

the promotive effect on cell growth induced by E2 was also significantly inhibited by SGLXD treatment. With the treatment of 1.70

3.40

5.10 mg/mL SGLXD

the nuclear ERα protein level was reduced to 88.1%

70.4% and 60.9% in MCF-7 cells

and was decreased to 43.0%

38.4% and 5.9% in ZR-75-1 cells as compared with the control group. In T47D cells

关键词

Breast Cancerestrogen receptorShugan Liangxue Decoctionestrogen receptor inhibitor

Keywords

Breast Cancerestrogen receptorShugan Liangxue Decoctionestrogen receptor inhibitor

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Key Laboratory of Internal Medicine, Hunan University of Traditional Chinese Medicine

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