Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia

Li-gong Zhang; Li-jun Wang; Qing-qing Shen; Hao-feng Wang; Ying Zhang; Cui-ge Shi; Shu-cheng Zhang; Meng-yuan Zhang

doi:10.1007/s11655-015-2124-3

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Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia

Updated：2021-08-27

- Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia
- Chinese Journal of Integrative Medicine Vol. 23, Issue 9, Pages: 696-702(2017)
- Affiliations：
  
  1. Department of Anesthesia, Shandong Provincial Hospital Affiliated to Shandong University,Jinan,China
  2. Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong University,Jinan,China
  3. The Fifth Hospital of Jinan,Jinan,China
  4. Department of Cell Biology, National Research Institute of Family Planning,Beijing,China
- Author bio：
- Funds：
  
  Supported by Excellent Adult and Young Scientist Science Foundation of Shandong Province (No. BS2010YY056) and the National Key Grant of Basic Research Project (No. 2010CB530403);Conflict of Interest;All the authors do not have any possible conficts of interest.;Author Contributions;Zhang LG, Wang LJ preformed the data analysis and wrote the manuscript;Zhang Y, Zhang SC, Shi CG helped conceive and design the experiments;Shen QQ and Wang HF performed the animal model preparation, behavioral test and daily drug intervening;Zhang MY helped perform the analysis with constructive discussions.
- DOI：10.1007/s11655-015-2124-3
  CLC：
- Published：2017，
  
  Published Online：17 November 2015，
Scan for full text
Zhang, Lg., Wang, Lj., Shen, Qq. et al. Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia., Chin. J. Integr. Med. 23, 696–702 (2017). https://doi.org/10.1007/s11655-015-2124-3

Li-gong Zhang, Li-jun Wang, Qing-qing Shen, et al. Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia. [J]. Chinese Journal of Integrative Medicine 23(9):696-702(2017)
Zhang, Lg., Wang, Lj., Shen, Qq. et al. Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia., Chin. J. Integr. Med. 23, 696–702 (2017). https://doi.org/10.1007/s11655-015-2124-3 DOI：

Li-gong Zhang, Li-jun Wang, Qing-qing Shen, et al. Paeoniflorin improves regional cerebral blood flow and suppresses inflammatory factors in the hippocampus of rats with vascular dementia. [J]. Chinese Journal of Integrative Medicine 23(9):696-702(2017) DOI： 10.1007/s11655-015-2124-3.

摘要

To explore the delayed neuroprotection induced by paeoniflorin (PF)

the principal component of Paeoniae radix prescribed in Chinese medicine

and its underlying mechanisms in rats subjected to vascular dementia (VD). A rat model of VD was induced by bilateral common carotid arteries occlusion (BCCAO). Low-dose or high-dose PF (20 or 40 mg/kg once per day) was administrated for 28 days after VD. The behavioral analysis of rat was measured by water morris. Regional cerebral blood volume (rCBV)

regional cerebral blood flflow (rCBF) and mean transit time (MTT) were measured in the bilateral hippocampus by perfusion-weighted imaging (PWI). The levels of interleukin-1β (IL-1β)

interleukin-6 (IL-6)

and tumor necrosis factor alpha (TNF-α) were measured by commercially available enzyme-linked immunosorbent assay kits. Protein levels were evaluated by western blot analysis. mRNA levels were evaluated by real time-polymerase chain reaction. Western blotting was used to estimate p65 translocation. The behavioral analysis showed that PF could decrease the escape latency time (P<0.05)

and increase the residence time of the original platform quadrant and the across platform frequency in water maze in VD rats (P<0.05). Likewise

PF remarkably promoted the rCBV (P<0.05)

rCBF and decreased per minute MTT (P<0.05) in hippocampus of VD rats. Furthermore

PF decreased the release of IL-1β

IL-6 and TNF-α as well as inhibited the mRNA expression of IL-1β

IL-6 and TNF-α in the hippocampus of VD rats (P<0.05 or P<0.01). PF also could decrease the protein expressions of inducible nitric oxide synthase and cyclooxygenase-2 in the hippocampus of VD rats (P<0.05 or P<0.01). In addition

PF signifificantly inhibited the nuclear factor κB (NF-κB) pathway in the hippocampus of VD rats. PF signifificantly attenuates cognitive impairment

improves hippocampus perfusion and inhibits inflflammatory response in VD rats. In addition

the anti-inflflammatory effects of PF might be due to inhibiting the NF-κB pathway. PF may be a potential clinical application in improving VD.

Abstract

To explore the delayed neuroprotection induced by paeoniflorin (PF)

the principal component of Paeoniae radix prescribed in Chinese medicine

regional cerebral blood flflow (rCBF) and mean transit time (MTT) were measured in the bilateral hippocampus by perfusion-weighted imaging (PWI). The levels of interleukin-1β (IL-1β)

interleukin-6 (IL-6)

and increase the residence time of the original platform quadrant and the across platform frequency in water maze in VD rats (P<0.05). Likewise

PF remarkably promoted the rCBV (P<0.05)

rCBF and decreased per minute MTT (P<0.05) in hippocampus of VD rats. Furthermore

PF decreased the release of IL-1β

IL-6 and TNF-α as well as inhibited the mRNA expression of IL-1β

PF signifificantly inhibited the nuclear factor κB (NF-κB) pathway in the hippocampus of VD rats. PF signifificantly attenuates cognitive impairment

improves hippocampus perfusion and inhibits inflflammatory response in VD rats. In addition

the anti-inflflammatory effects of PF might be due to inhibiting the NF-κB pathway. PF may be a potential clinical application in improving VD.

关键词

paeoniflflorinVascular Dementiahippocampuscerebral blood flflow

Keywords

paeoniflflorinVascular Dementiahippocampuscerebral blood flflow

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