San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway

Shi-zhang Wei; Sheng-qiang Luo; Jian Wang; Jia-bo Wang; Rui-sheng Li; Xiao-mei Zhang; Yan-lei Guo; Chang Chen; Xiao Ma; Zhe Chen; Hong-hong Liu; Zhi-rui Yang; Jian-yu Li; Rui-lin Wang; Ya-ming Zhang; Hui-yin Yang; Xiao-he Xiao; Yan-ling Zhao

doi:10.1007/s11655-015-2127-0

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San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway

Updated：2021-08-27

- San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway
- Chinese Journal of Integrative Medicine Vol. 24, Issue 7, Pages: 502-511(2018)
- Affiliations：
  
  1. College of Pharmacy, Chengdu University of Traditional Chinese Medicine,Chengdu,China
  2. Department of Pharmacy, Hospital of People’s Liberation Army,Beijing,China,302
  3. Department of Integrative Medical Center, Hospital of People’s Liberation Army,Beijing,China,302
  4. China Military Institute of Chinese Medicine, Hospital of People’s Liberation Army,Beijing,China,302
  5. Animal Laboratory Center, Hospital of People’s Liberation Army,Beijing,China,302
  6. Chongqing Academy of Chinese Traditional Materia Medica, Key Laboratory of Chongqing Traditional Chinese Medicine Resources,Chongqing,China
- Author bio：
- Funds：
  
  Supported by the Major Projects of the National Science and Technology (No. 2012ZX10005010-002-002) and the National Natural Science Foundation of China (No. 81303120 and No. 81173571)
- DOI：10.1007/s11655-015-2127-0
  CLC：
- Published：2018，
  
  Published Online：19 December 2015，
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Wei, Sz., Luo, Sq., Wang, J. et al. San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway., Chin. J. Integr. Med. 24, 502–511 (2018). https://doi.org/10.1007/s11655-015-2127-0

Shi-zhang Wei, Sheng-qiang Luo, Jian Wang, et al. San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway. [J]. Chinese Journal of Integrative Medicine 24(7):502-511(2018)
Wei, Sz., Luo, Sq., Wang, J. et al. San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway., Chin. J. Integr. Med. 24, 502–511 (2018). https://doi.org/10.1007/s11655-015-2127-0 DOI：

Shi-zhang Wei, Sheng-qiang Luo, Jian Wang, et al. San-Cao Granule (三草颗粒) Ameliorates Hepatic Fibrosis through High Mobility Group Box-1 Protein/Smad Signaling Pathway. [J]. Chinese Journal of Integrative Medicine 24(7):502-511(2018) DOI： 10.1007/s11655-015-2127-0.

摘要

To investigate the possible mechanism of San-Cao Granule (SCG

三草颗粒) mediating antiliver fibrosis. A total of 60 male Sprague-Dawley rats were randomly divided into the normal control group

porcine serum-treated group

ursodesoxycholic acid (UDCA

60 mg/kg)

SCG (3.6 g/kg) group

SCG (1.8 g/kg) group and SCG (0.9 g/kg) group

with 10 rats in each group. Liver fibrosis was induced with porcine serum by intraperitoneal injection for 8 weeks

except for the normal control group. Then

the rats in the three SCG-treated groups and UDCA group were administered SCG and UDCA respectively for 4 weeks. The serum levels of alanine transaminase (ALT)

aspartate transaminase (AST)

albumin (ALB)

total bilirubin (TBIL)

hyaluronic acid (HA)

laminin (LN)

and type IV collagen (IVC) were examined using commercial kits and hepatic histopathology was examined with hematoxylin and eosin and Masson staining. Moreover

the protein expression levels of high mobility group box-1 protein (HMGB1)

transforming growth factor β1 (TGF-β1)

phosphorylated mothers against decapentaplegic homolog 3 (p-Smad3)

Smad7

toll-like receptor 4 (TLR4)

myeloid differentiation factor 88 (MyD88)

nuclear factor-kappa B (NF-κB) and α-smooth muscle actin (α-SMA) were determined by western blot

immunohistochemistry and real time quantitative-reverse transcription polymerase. Both SCG (3.6 and 1.8 g/kg) and UDCA significantly ameliorated the liver fibrosis induced by porcine serum as indicated by retarding the serum levels increasing of ALT

AST

TBIL

LN and IVC and preventing the serum level reducing of ALB compared with the model group (all P<0.01). Meanwhile

the collagen deposition was attenuated by SCG and UDCA treatment. Furthermore

SCG markedly reduced the expressions of HMGB1

TGF-β1

p-Smad3

TLR4

MyD88

NF-κB and α-SMA

and enhanced the expression of the Smad7 compared with the model group (all P<0.01). SCG ameliorates hepatic fibrosis possibly through inhibiting HMGB1

TLR4/NF-κB and TGF-β1/Smad signaling pathway.

Abstract

To investigate the possible mechanism of San-Cao Granule (SCG

三草颗粒) mediating antiliver fibrosis. A total of 60 male Sprague-Dawley rats were randomly divided into the normal control group

porcine serum-treated group

ursodesoxycholic acid (UDCA

60 mg/kg)

SCG (3.6 g/kg) group

SCG (1.8 g/kg) group and SCG (0.9 g/kg) group

with 10 rats in each group. Liver fibrosis was induced with porcine serum by intraperitoneal injection for 8 weeks

except for the normal control group. Then

the rats in the three SCG-treated groups and UDCA group were administered SCG and UDCA respectively for 4 weeks. The serum levels of alanine transaminase (ALT)

aspartate transaminase (AST)

albumin (ALB)

total bilirubin (TBIL)

hyaluronic acid (HA)

laminin (LN)

and type IV collagen (IVC) were examined using commercial kits and hepatic histopathology was examined with hematoxylin and eosin and Masson staining. Moreover

the protein expression levels of high mobility group box-1 protein (HMGB1)

transforming growth factor β1 (TGF-β1)

phosphorylated mothers against decapentaplegic homolog 3 (p-Smad3)

Smad7

toll-like receptor 4 (TLR4)

myeloid differentiation factor 88 (MyD88)

nuclear factor-kappa B (NF-κB) and α-smooth muscle actin (α-SMA) were determined by western blot

AST

TBIL

LN and IVC and preventing the serum level reducing of ALB compared with the model group (all P<0.01). Meanwhile

the collagen deposition was attenuated by SCG and UDCA treatment. Furthermore

SCG markedly reduced the expressions of HMGB1

TGF-β1

p-Smad3

TLR4

MyD88

NF-κB and α-SMA

and enhanced the expression of the Smad7 compared with the model group (all P<0.01). SCG ameliorates hepatic fibrosis possibly through inhibiting HMGB1

TLR4/NF-κB and TGF-β1/Smad signaling pathway.

关键词

San-Cao GranuleLiver Fibrosishigh mobility group box-1 proteintoll-like receptor 4/nuclear factor-kappa Btransforming growth factor β1/mothers against decapentaplegic homolog

Keywords

San-Cao GranuleLiver Fibrosishigh mobility group box-1 proteintoll-like receptor 4/nuclear factor-kappa Btransforming growth factor β1/mothers against decapentaplegic homolog

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Key Laboratory of Liver and Kidney Diseases, Shanghai University of Traditional Chinese Medicine

Institute of Traditional Chinese Internal Medicine, Shanghai Municipal Education Commission, Shanghai

Institute of Liver Diseases, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Institute of Liver Diseases, Shanghai University of Chinese medicine

Department of Gastroenterology, Shanghai East Hospital Affiliated to Tongji University

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