Potent therapeutic effects of Shouwu Jiangqi Decoction (首乌僵芪汤) on polycystic ovary syndrome with insulin resistance in rats

Li-hong Wang; Xu Wang; Xi-zhong Yu; Wen-ting Xu

doi:10.1007/s11655-015-2147-9

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Potent therapeutic effects of Shouwu Jiangqi Decoction (首乌僵芪汤) on polycystic ovary syndrome with insulin resistance in rats

Updated：2021-08-27

- Potent therapeutic effects of Shouwu Jiangqi Decoction (首乌僵芪汤) on polycystic ovary syndrome with insulin resistance in rats
- Chinese Journal of Integrative Medicine Vol. 22, Issue 2, Pages: 116-123(2016)
- Affiliations：
  
  1. Zhangjiagang Hospital of Chinese Medicine, Zhangjiagang,Jiangsu Province,China
  2. The First Clinical College, Nanjing University of Chinese Medicine,Nanjing,China
- Author bio：
- Funds：
  
  Supported by the Science and Technology Project of Traditional Chinese Medicines of Jiangsu Province in 2011–2012 (No. LZ11153) and Innovation Research Project for postgraduates in Colleges of Jiangsu Province in 2011(No. CXZZ11_0780).
- DOI：10.1007/s11655-015-2147-9
  CLC：
- Published：2016，
  
  Published Online：16 July 2015，
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Wang, Lh., Wang, X., Yu, Xz. et al. Potent therapeutic effects of Shouwu Jiangqi Decoction (首乌僵芪汤) on polycystic ovary syndrome with insulin resistance in rats., Chin. J. Integr. Med. 22, 116–123 (2016). https://doi.org/10.1007/s11655-015-2147-9

Li-hong Wang, Xu Wang, Xi-zhong Yu, et al. Potent therapeutic effects of Shouwu Jiangqi Decoction (首乌僵芪汤) on polycystic ovary syndrome with insulin resistance in rats. [J]. Chinese Journal of Integrative Medicine 22(2):116-123(2016)
Wang, Lh., Wang, X., Yu, Xz. et al. Potent therapeutic effects of Shouwu Jiangqi Decoction (首乌僵芪汤) on polycystic ovary syndrome with insulin resistance in rats., Chin. J. Integr. Med. 22, 116–123 (2016). https://doi.org/10.1007/s11655-015-2147-9 DOI：

Li-hong Wang, Xu Wang, Xi-zhong Yu, et al. Potent therapeutic effects of Shouwu Jiangqi Decoction (首乌僵芪汤) on polycystic ovary syndrome with insulin resistance in rats. [J]. Chinese Journal of Integrative Medicine 22(2):116-123(2016) DOI： 10.1007/s11655-015-2147-9.

摘要

To investigate the effect of Shouwu Jiangqi Decoction (首乌僵芪汤

SJD) on polycystic ovary syndrome (PCOS) with insulin resistance (IR) in rats and to explore the underlining molecular mechanisms. A total of 51 female Sprague-Dawley rats were randomly divided into 6 groups: control group (n=7)

model group (n=8)

SJD high-dose group (n=9)

SJD medium-dose group (n=9)

SJD low-dose group (n=9) and DMBG group (n=9). Radioimmunoassay was used to measure serum follicle-stimulating hormone (FSH)

luteinizing hormone (LH) and testosterone concentrations and qRT-PCR and western blot were used to examine the expression levels of mRNA and protein respectively of insulin receptor substrate 1 (IRS-1) and phosphatidylinositide 3-kinases (PI3K) p85α in different groups. FSH level signififi cantly decreased in the model group compared with the normal control (P<0.01)

and high-dose SJD and DMBG can signififi cantly increase FSH level (P<0.01). LH level showed a mild increase without statistic signififi cance in the model group compared with the control and different dosages of SJD had no signififi cance effect on LH level

while DMBG can signififi cantly decrease LH level (P<0.01). Testosterone level signififi cantly increased in the model group compared with the control group (P<0.01)

and high-dose SJD and DMBG can signififi cantly decrease testosterone level (P<0.01). The expression of IRS-1 as well as PI3Kp85α were significantly decreased in the model group compared with the normal control group at both mRNA (P<0.001) and protein (P<0.01) level

and both high-dose SJD and DMBG can enhance IRS-1 and PI3K expression (P<0.05). SJD has potent therapeutic effects on PCOS with IR in rats. The therapeutic effects of SJD on IR and ovulatory dysfunction are probably achieved through correcting the defective insulin signaling transduction.

Abstract

To investigate the effect of Shouwu Jiangqi Decoction (首乌僵芪汤

model group (n=8)

SJD high-dose group (n=9)

SJD medium-dose group (n=9)

SJD low-dose group (n=9) and DMBG group (n=9). Radioimmunoassay was used to measure serum follicle-stimulating hormone (FSH)

while DMBG can signififi cantly decrease LH level (P<0.01). Testosterone level signififi cantly increased in the model group compared with the control group (P<0.01)

关键词

polycystic ovary syndromeinsulin resistanceinsulin signaling pathwayChinese Medicine

Keywords

polycystic ovary syndromeinsulin resistanceinsulin signaling pathwayChinese Medicine

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