Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice

Lin Yang; Ming-wei Yu; Xiao-min Wang; Yi Zhang; Guo-wang Yang; Xiao-qin Luo; Rui-yun Peng; Ya-bing Gao; Li Zhao; Li-feng Wang

doi:10.1007/s11655-015-2152-z

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Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice

Updated：2021-08-27

- Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice
- Chinese Journal of Integrative Medicine Vol. 22, Issue 2, Pages: 110-115(2016)
- Affiliations：
  
  1. Department of Oncology, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University,Beijing,China
  2. Institute of Radiation Medicine, Academy of Military Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30973745)
- DOI：10.1007/s11655-015-2152-z
  CLC：
- Published：2016，
  
  Published Online：4 July 2015，
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Yang, L., Yu, Mw., Wang, Xm. et al. Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice., Chin. J. Integr. Med. 22, 110–115 (2016). https://doi.org/10.1007/s11655-015-2152-z

Lin Yang, Ming-wei Yu, Xiao-min Wang, et al. Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice. [J]. Chinese Journal of Integrative Medicine 22(2):110-115(2016)
Yang, L., Yu, Mw., Wang, Xm. et al. Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice., Chin. J. Integr. Med. 22, 110–115 (2016). https://doi.org/10.1007/s11655-015-2152-z DOI：

Lin Yang, Ming-wei Yu, Xiao-min Wang, et al. Heijiangdan Ointment (黑绛丹膏) relieves oxidative stress from radiation dermatitis induced by 60Co γ-ray in mice. [J]. Chinese Journal of Integrative Medicine 22(2):110-115(2016) DOI： 10.1007/s11655-015-2152-z.

摘要

To investigate the effects of Heijiangdan Ointment (黑绛丹膏

HJD) on oxidative stress in 60Co γ-ray radiation-induced dermatitis in mice. Female Wistar mice with grade 4 radiation dermatitis induced by 60Co γ-rays were randomly divided into four groups (n=12 per group); the HJD-treated

recombinant human epidermal growth factor (rhEGF)-treated

Trolox-treated

and untreated groups

along with a negative control group. On the 11th and 21st days after treatment

6 mice in each group were chosen for evaluation. The levels of superoxide dismutase (SOD)

malondialdehyde (MDA)

and lactate dehydrogenase (LDH) were detected using spectrophotometric methods. The fibroblast mitochondria were observed by transmission electron microscopy (TEM). The expressions of fibroblast growth factor 2 (FGF-2) and transforming growth factor β1 (TGF-β1) were analyzed by western blot. Compared with the untreated group

the levels of SOD

MDA and LDH

on the 11th and 21st days after treatment showed significant difference (P<0.05). TEM analysis indicated that fibroblast mitochondria in the untreated group exhibited swelling and the cristae appeared fractured

while in the HJD group

the swelling of mitochondria was limited and the rough endoplasmic reticulum appeared more relaxed. The expressions of FGF-2 and TGF-β1 increased in the untreated group compared with the negative control group (P<0.05). After treatment

the expression of FGF-2

rhEGF and Trolox in the HJD group were significantly increased compared with the untreated group (P<0.05)

or compared with the negative control group (P<0.05). The expression of TGF-β1 showed significant difference between untreated and negative control groups (P<0.05). HJD and Trolox increased the level of TGF-β1 and the difference was marked as compared with the untreated and negative control groups (P<0.05). HJD relieves oxidative stress-induced injury

increases the antioxidant activity

mitigates the fibroblast mitochondrial damage

up-regulates the expression of growth factor

and promotes mitochondrial repair in mice.

Abstract

To investigate the effects of Heijiangdan Ointment (黑绛丹膏

recombinant human epidermal growth factor (rhEGF)-treated

Trolox-treated

and untreated groups

along with a negative control group. On the 11th and 21st days after treatment

6 mice in each group were chosen for evaluation. The levels of superoxide dismutase (SOD)

malondialdehyde (MDA)

the levels of SOD

MDA and LDH

while in the HJD group

the expression of FGF-2

rhEGF and Trolox in the HJD group were significantly increased compared with the untreated group (P<0.05)

increases the antioxidant activity

mitigates the fibroblast mitochondrial damage

up-regulates the expression of growth factor

and promotes mitochondrial repair in mice.

关键词

Chinese MedicineHeijiangdan Ointmentradiation dermatitisoxidative stressantioxidantfibroblast mitochondriatransmission electron microscopegrowth factor

Keywords

Chinese MedicineHeijiangdan Ointmentradiation dermatitisoxidative stressantioxidantfibroblast mitochondriatransmission electron microscopegrowth factor

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